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Yorodumi- PDB-2ho9: Solution Structure of chemotaxi protein CheW from Escherichia coli -
+Open data
-Basic information
Entry | Database: PDB / ID: 2ho9 | ||||||
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Title | Solution Structure of chemotaxi protein CheW from Escherichia coli | ||||||
Components | Chemotaxis protein cheW | ||||||
Keywords | SIGNALING PROTEIN / chemotaxis protein CheW / Escherichia coli / solution structure | ||||||
Function / homology | Function and homology information methyl accepting chemotaxis protein complex / positive regulation of post-translational protein modification / cell tip / aerotaxis / establishment of localization in cell / chemotaxis / protein domain specific binding / signal transduction / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | SOLUTION NMR / simulated annealing, molecular dynamics | ||||||
Authors | Jin, C. / Li, Y. | ||||||
Citation | Journal: To be Published Title: Solution Structure of chemotaxi protein CheW from Escherichia coli Authors: Jin, C. / Li, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2ho9.cif.gz | 1001.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2ho9.ent.gz | 871.1 KB | Display | PDB format |
PDBx/mmJSON format | 2ho9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ho/2ho9 ftp://data.pdbj.org/pub/pdb/validation_reports/ho/2ho9 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 18095.693 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Plasmid: pET21a / Production host: Escherichia coli (E. coli) / References: UniProt: P0A964 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 3D heteronuclear techniques |
-Sample preparation
Details | Contents: 1mM ArsC U-15N, 13C; 30mM Tris 30mM NaCl buffer; 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 60mM / pH: 7.3 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing, molecular dynamics / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |