- PDB-2hfq: NMR structure of protein NE1680 from Nitrosomonas europaea: North... -
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基本情報
登録情報
データベース: PDB / ID: 2hfq
タイトル
NMR structure of protein NE1680 from Nitrosomonas europaea: Northeast Structural Genomics Consortium target NeT5
要素
Hypothetical protein
キーワード
STRUCTURAL GENOMICS / UNKNOWN FUNCTION / a/b protein / PSI-2 / Protein Structure Initiative / Northeast Structural Genomics Consortium / NESG
機能・相同性
NE1680-like / Protein of unknown function DUF2024 / NE1680-like superfamily / Domain of unknown function (DUF2024) / NE1680-like fold / Roll / Alpha Beta / Uncharacterized protein
機能・相同性情報
生物種
Nitrosomonas europaea (バクテリア)
手法
溶液NMR / The structure was determined using AutoStructure, refined manually
Group: Data collection / カテゴリ: chem_comp_atom / chem_comp_bond
Remark 999
Sequence The authors state that the sample contains an apparent mutation at position 61, where the ... Sequence The authors state that the sample contains an apparent mutation at position 61, where the reported glutamine was found to be glutamic acid.
Text: The sample contains a 22 residue N-term tag, and a two residue C-term cloning artifact. No coordinates for these unstructured residues were included. The sample also contains an apparent ...Text: The sample contains a 22 residue N-term tag, and a two residue C-term cloning artifact. No coordinates for these unstructured residues were included. The sample also contains an apparent mutation at position 61, where the reported glutamine was found to be glutamic acid.
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試料調製
詳細
Solution-ID
内容
溶媒系
1
1 mM NE1680 U-13C,15N; 10mM MOPS, 450 mM NaCl, 10 mM DTT, 0.01% NaN3, pH 6.5, 90% H20, 10% D2O
90% H20, 10% D2O
2
1 mM NE1680 U-13C,15N; 10mM MOPS, 450 mM NaCl, 10 mM DTT, 0.01% NaN3, pH 6.5, 100% D2O
100% D2O
試料状態
イオン強度: 450 mM NaCl / pH: 6.5 / 圧: ambient / 温度: 298 K