- PDB-2hfq: NMR structure of protein NE1680 from Nitrosomonas europaea: North... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 2hfq
Title
NMR structure of protein NE1680 from Nitrosomonas europaea: Northeast Structural Genomics Consortium target NeT5
Components
Hypothetical protein
Keywords
STRUCTURAL GENOMICS / UNKNOWN FUNCTION / a/b protein / PSI-2 / Protein Structure Initiative / Northeast Structural Genomics Consortium / NESG
Function / homology
NE1680-like / Protein of unknown function DUF2024 / NE1680-like superfamily / Domain of unknown function (DUF2024) / NE1680-like fold / Roll / Alpha Beta / DUF2024 domain-containing protein
Function and homology information
Biological species
Nitrosomonas europaea (bacteria)
Method
SOLUTION NMR / The structure was determined using AutoStructure, refined manually
Group: Data collection / Category: chem_comp_atom / chem_comp_bond
Remark 999
Sequence The authors state that the sample contains an apparent mutation at position 61, where the ... Sequence The authors state that the sample contains an apparent mutation at position 61, where the reported glutamine was found to be glutamic acid.
Mass: 12537.901 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Nitrosomonas europaea (bacteria) / Gene: NE1680 / Plasmid: pET15b derivative / Production host: Escherichia coli (E. coli) / Strain (production host): Novagen BL21(DE3) Gold / References: UniProt: Q82U33
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
3D 13C-separated NOESY
1
2
1
3D 15N-separated NOESY
1
3
2
3D 13C-separated NOESY
1
4
2
4D 13C-separated NOESY
NMR details
Text: The sample contains a 22 residue N-term tag, and a two residue C-term cloning artifact. No coordinates for these unstructured residues were included. The sample also contains an apparent ...Text: The sample contains a 22 residue N-term tag, and a two residue C-term cloning artifact. No coordinates for these unstructured residues were included. The sample also contains an apparent mutation at position 61, where the reported glutamine was found to be glutamic acid.
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
1 mM NE1680 U-13C,15N; 10mM MOPS, 450 mM NaCl, 10 mM DTT, 0.01% NaN3, pH 6.5, 90% H20, 10% D2O
90% H20, 10% D2O
2
1 mM NE1680 U-13C,15N; 10mM MOPS, 450 mM NaCl, 10 mM DTT, 0.01% NaN3, pH 6.5, 100% D2O
100% D2O
Sample conditions
Ionic strength: 450 mM NaCl / pH: 6.5 / Pressure: ambient / Temperature: 298 K
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NMR measurement
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
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