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Yorodumi- PDB-2hfh: THE NMR STRUCTURES OF A WINGED HELIX PROTEIN: GENESIS, 20 STRUCTURES -
+Open data
-Basic information
Entry | Database: PDB / ID: 2hfh | ||||||
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Title | THE NMR STRUCTURES OF A WINGED HELIX PROTEIN: GENESIS, 20 STRUCTURES | ||||||
Components | GENESIS | ||||||
Keywords | HNF-3 HOMOLOGUES / WINGED HELIX PROTEIN | ||||||
Function / homology | Function and homology information trophectodermal cell differentiation / embryonic placenta development / cellular response to leukemia inhibitory factor / RNA polymerase II transcription regulatory region sequence-specific DNA binding / DNA-binding transcription repressor activity, RNA polymerase II-specific / sequence-specific double-stranded DNA binding / double-stranded DNA binding / in utero embryonic development / transcription cis-regulatory region binding / RNA polymerase II cis-regulatory region sequence-specific DNA binding ...trophectodermal cell differentiation / embryonic placenta development / cellular response to leukemia inhibitory factor / RNA polymerase II transcription regulatory region sequence-specific DNA binding / DNA-binding transcription repressor activity, RNA polymerase II-specific / sequence-specific double-stranded DNA binding / double-stranded DNA binding / in utero embryonic development / transcription cis-regulatory region binding / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / regulation of DNA-templated transcription / chromatin / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / nucleus Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | SOLUTION NMR / DIANA | ||||||
Authors | Marsden, I. / Jin, C. / Liao, X. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1998 Title: Structural changes in the region directly adjacent to the DNA-binding helix highlight a possible mechanism to explain the observed changes in the sequence-specific binding of winged helix proteins. Authors: Marsden, I. / Jin, C. / Liao, X. #1: Journal: Biochemistry / Year: 1997 Title: Evidence that the DNA Binding Specificity of Winged Helix Proteins is Mediated by a Structural Change in the Amino Acid Sequence Adjacent to the Principal DNA Binding Helix Authors: Marsden, I. / Chen, Y. / Jin, C. / Liao, X. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2hfh.cif.gz | 586.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2hfh.ent.gz | 492.8 KB | Display | PDB format |
PDBx/mmJSON format | 2hfh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2hfh_validation.pdf.gz | 345.2 KB | Display | wwPDB validaton report |
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Full document | 2hfh_full_validation.pdf.gz | 537.9 KB | Display | |
Data in XML | 2hfh_validation.xml.gz | 41.2 KB | Display | |
Data in CIF | 2hfh_validation.cif.gz | 61.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hf/2hfh ftp://data.pdbj.org/pub/pdb/validation_reports/hf/2hfh | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13049.021 Da / Num. of mol.: 1 / Fragment: DNA-BINDING DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Description: FIRST PUBLISHED IN PNAS 90, 3948-3952 / Cell line: 293 / Cellular location: NUCLEUS / Gene: HFH-2, GENESIS / Organ: LIVER, LUNG, HEART / Plasmid: PET21 / Gene (production host): T7 / Organelle (production host): INCLUSION BODIES / Production host: Escherichia coli (E. coli) / Strain (production host): HMS-174 / References: UniProt: Q63245 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Sample conditions | pH: 6.5 / Temperature: 290 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: DIANA / Software ordinal: 1 | |||||||||
NMR ensemble | Conformer selection criteria: LOWEST TARGET FUNCTION / Conformers calculated total number: 100 / Conformers submitted total number: 20 |