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- PDB-2gvb: REFINED SOLUTION STRUCTURE OF THE TYR 41--> HIS MUTANT OF THE M13... -

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Entry
Database: PDB / ID: 2gvb
TitleREFINED SOLUTION STRUCTURE OF THE TYR 41--> HIS MUTANT OF THE M13 GENE V PROTEIN. A COMPARISON WITH THE CRYSTAL STRUCTURE
ComponentsGENE V PROTEIN
KeywordsDNA-BINDING (VIRAL)
Function / homology
Function and homology information


rolling circle single-stranded viral DNA replication / single-stranded DNA binding / DNA replication
Similarity search - Function
Bacteriophage M13, G5P, DNA-binding / Helix-destabilising protein / Nucleic acid-binding proteins / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Nucleic acid-binding, OB-fold / Beta Barrel / Mainly Beta
Similarity search - Domain/homology
DNA-Binding protein G5P
Similarity search - Component
Biological speciesEnterobacteria phage M13 (virus)
MethodSOLUTION NMR
AuthorsFolkers, P.J.M. / Nilges, M. / Folmer, R.H.A. / Prompers, J.J. / Konings, R.N.H. / Hilbers, C.W.
Citation
Journal: Eur.J.Biochem. / Year: 1995
Title: Refined solution structure of the Tyr41-->His mutant of the M13 gene V protein. A comparison with the crystal structure.
Authors: Prompers, J.J. / Folmer, R.H. / Nilges, M. / Folkers, P.J. / Konings, R.N. / Hilbers, C.W.
#1: Journal: J.Mol.Biol. / Year: 1994
Title: The Solution Structure of the Tyr 41--> His Mutant of the Single-Stranded DNA Binding Protein Encoded by Gene V of the Filamentous Bacteriophage M13
Authors: Folkers, P.J.M. / Nilges, M. / Folmer, R.H.A. / Konings, R.N.H. / Hilbers, C.W.
#2: Journal: Biochemistry / Year: 1993
Title: Exploring the DNA Binding Domain of Gene V Protein Encoded by Bacteriophage M13 with the Aid of Spin-Labeled Oligonucleotides in Combination with H-NMR
Authors: Folkers, P.J.M. / Van Duynhoven, J.P.M. / Van Lieshout, H.T.M. / Harmsen, B.J.M. / Van Boom, J.H. / Tesser, G.I. / Konings, R.N.H. / Hilbers, C.W.
#3: Journal: Eur.J.Biochem. / Year: 1991
Title: Sequence-Specific H-NMR Assignment and Secondary Structure of the Tyr 41--> His Mutant of the Single-Stranded DNA Binding Protein, Gene V Protein, Encoded by the Filamentous Bacteriophage M13
Authors: Folkers, P.J.M. / Van Duynhoven, J.P.M. / Jonker, A.J. / Harmsen, B.J.M. / Konings, R.N.H. / Hilbers, C.W.
#4: Journal: Eur.J.Biochem. / Year: 1991
Title: Characterization of Wild-Type and Mutant M13 Gene V Proteins by Means of H-NMR
Authors: Folkers, P.J.M. / Stassen, A.P.M. / Van Duynhoven, J.P.M. / Harmsen, B.J.M. / Konings, R.N.H. / Hilbers, C.W.
History
DepositionJul 27, 1995Processing site: BNL
SupersessionOct 15, 1995ID: 1GVB
Revision 1.0Oct 15, 1995Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Mar 9, 2022Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site / _struct_ref_seq_dif.details
Revision 1.4May 29, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: GENE V PROTEIN
B: GENE V PROTEIN


Theoretical massNumber of molelcules
Total (without water)19,3482
Polymers19,3482
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)1 / -
Representative

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Components

#1: Protein GENE V PROTEIN


Mass: 9674.188 Da / Num. of mol.: 2 / Mutation: Y41H
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Enterobacteria phage M13 (virus) / Genus: Inovirus / Plasmid: M13 / References: UniProt: P69544

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Sample preparation

Crystal grow
*PLUS
Method: other / Details: NMR

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Processing

Software
NameClassification
X-PLORmodel building
X-PLORrefinement
X-PLORphasing
NMR software
NameDeveloperClassification
DIANAGUNTERT,BRAUN,WUTHRICHrefinement
X-PLORBRUNGERrefinement
NMR ensembleConformers submitted total number: 1

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