+Open data
-Basic information
Entry | Database: PDB / ID: 2gtj | ||||||
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Title | Reduced form of ADAP hSH3-N-domain | ||||||
Components | FYN-binding protein | ||||||
Keywords | SIGNALING PROTEIN / SH3 / redox | ||||||
Function / homology | Function and homology information anchoring junction / Signal regulatory protein family interactions / Generation of second messenger molecules / protein localization to plasma membrane / integrin-mediated signaling pathway / actin cytoskeleton / T cell receptor signaling pathway / immune response / signaling receptor binding / lipid binding ...anchoring junction / Signal regulatory protein family interactions / Generation of second messenger molecules / protein localization to plasma membrane / integrin-mediated signaling pathway / actin cytoskeleton / T cell receptor signaling pathway / immune response / signaling receptor binding / lipid binding / protein-containing complex binding / protein-containing complex / nucleus / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Zimmermann, J. / Kuehne, R. / Freund, C. | ||||||
Citation | Journal: Biochemistry / Year: 2007 Title: Redox-Regulated Conformational Changes in an SH3 Domain Authors: Zimmermann, J. / Kuhne, R. / Sylvester, M. / Freund, C. #1: Journal: J.Biomol.Nmr / Year: 2005 Title: NMR assignment of the reduced and oxidized forms of the human ADAP hSH3-1 domain. Authors: Zimmermann, J. / Freund, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2gtj.cif.gz | 612 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2gtj.ent.gz | 514.6 KB | Display | PDB format |
PDBx/mmJSON format | 2gtj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2gtj_validation.pdf.gz | 347.5 KB | Display | wwPDB validaton report |
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Full document | 2gtj_full_validation.pdf.gz | 490.8 KB | Display | |
Data in XML | 2gtj_validation.xml.gz | 34.5 KB | Display | |
Data in CIF | 2gtj_validation.cif.gz | 57.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gt/2gtj ftp://data.pdbj.org/pub/pdb/validation_reports/gt/2gtj | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11061.803 Da / Num. of mol.: 1 / Fragment: hSH3-N (reduced) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: FYB, SLAP130 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 DE3 / References: UniProt: O15117 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1mM hSH3-N, 20 mM sodium phosphate, 150 mM NaCl, pH 7.4 Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 150 mM / pH: 7.4 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: structures were calculated with XPLOR-NIH, the final structures were subjected to water refinement with XPLOR-NIH | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |