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- PDB-2ghp: Crystal structure of the N-terminal 3 RNA binding domains of the ... -

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Basic information

Entry
Database: PDB / ID: 2ghp
TitleCrystal structure of the N-terminal 3 RNA binding domains of the yeast splicing factor Prp24
ComponentsU4/U6 snRNA-associated splicing factor PRP24
KeywordsRNA BINDING PROTEIN / RNA chaperone / RNA binding domain / RNA recognition motif / splicing factor / snRNP / spliceosome / Structural Genomics / Protein Structure Initiative / PSI / Center for Eukaryotic Structural Genomics / CESG
Function / homology
Function and homology information


U6 snRNP / snRNA binding / spliceosomal complex assembly / spliceosomal tri-snRNP complex assembly / U6 snRNA binding / spliceosomal complex / mRNA splicing, via spliceosome / ribonucleoprotein complex / RNA binding / nucleus
Similarity search - Function
Occluded RNA-recognition motif / Prp24, RNA recognition motif1 / Prp24, RNA recognition motif2 / : / Occluded RNA-recognition motif / LSM-interacting domain / Lsm interaction motif / RRM (RNA recognition motif) domain / RNA recognition motif / RNA recognition motif ...Occluded RNA-recognition motif / Prp24, RNA recognition motif1 / Prp24, RNA recognition motif2 / : / Occluded RNA-recognition motif / LSM-interacting domain / Lsm interaction motif / RRM (RNA recognition motif) domain / RNA recognition motif / RNA recognition motif / Eukaryotic RNA Recognition Motif (RRM) profile. / RNA recognition motif domain / RNA-binding domain superfamily / Nucleotide-binding alpha-beta plait domain superfamily / Alpha-Beta Plaits / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
U4/U6 snRNA-associated-splicing factor PRP24
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
MethodX-RAY DIFFRACTION / SYNCHROTRON / SAD / MAD / Resolution: 2.7 Å
AuthorsBae, E. / Wesenberg, G.E. / Phillips Jr., G.N. / Bitto, E. / Bingman, C.A. / Center for Eukaryotic Structural Genomics (CESG)
CitationJournal: J.Mol.Biol. / Year: 2007
Title: Structure and interactions of the first three RNA recognition motifs of splicing factor prp24.
Authors: Bae, E. / Reiter, N.J. / Bingman, C.A. / Kwan, S.S. / Lee, D. / Phillips Jr., G.N. / Butcher, S.E. / Brow, D.A.
History
DepositionMar 27, 2006Deposition site: RCSB / Processing site: RCSB
Revision 1.0Apr 25, 2006Provider: repository / Type: Initial release
Revision 1.1Jan 15, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Advisory / Version format compliance
Revision 1.3Oct 18, 2017Group: Refinement description / Category: software
Revision 1.4Oct 9, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_entry_details / pdbx_modification_feature / struct_conn / struct_ncs_dom_lim / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_leaving_atom_flag / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ref_seq_dif.details

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: U4/U6 snRNA-associated splicing factor PRP24
B: U4/U6 snRNA-associated splicing factor PRP24
C: U4/U6 snRNA-associated splicing factor PRP24
D: U4/U6 snRNA-associated splicing factor PRP24
E: U4/U6 snRNA-associated splicing factor PRP24
F: U4/U6 snRNA-associated splicing factor PRP24
G: U4/U6 snRNA-associated splicing factor PRP24
H: U4/U6 snRNA-associated splicing factor PRP24


Theoretical massNumber of molelcules
Total (without water)267,2118
Polymers267,2118
Non-polymers00
Water3,477193
1
A: U4/U6 snRNA-associated splicing factor PRP24
B: U4/U6 snRNA-associated splicing factor PRP24
C: U4/U6 snRNA-associated splicing factor PRP24
D: U4/U6 snRNA-associated splicing factor PRP24


Theoretical massNumber of molelcules
Total (without water)133,6054
Polymers133,6054
Non-polymers00
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
E: U4/U6 snRNA-associated splicing factor PRP24
F: U4/U6 snRNA-associated splicing factor PRP24
G: U4/U6 snRNA-associated splicing factor PRP24
H: U4/U6 snRNA-associated splicing factor PRP24


Theoretical massNumber of molelcules
Total (without water)133,6054
Polymers133,6054
Non-polymers00
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)114.608, 125.848, 196.542
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11A
21E
31A
41E
51A
61E
71A
81E
91A
101E
12B
22F
32B
42F
52B
62F
72B
82F
92B
102F
13C
23G
33C
43G
53C
63G
73C
83G
93C
103G
14D
24H
34D
44H
54D
64H
74D
84H
94D
104H

NCS domain segments:

Refine code: 2

Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111THRTHRVALVALAA42 - 10343 - 104
211THRTHRVALVALEE42 - 10343 - 104
321GLUGLULEULEUAA108 - 149109 - 150
421GLUGLULEULEUEE108 - 149109 - 150
531PHEPHEPROPROAA160 - 195161 - 196
631PHEPHEPROPROEE160 - 195161 - 196
741LEULEUPROPROAA207 - 243208 - 244
841LEULEUPROPROEE207 - 243208 - 244
951CYSCYSASPASPAA254 - 288255 - 289
1051CYSCYSASPASPEE254 - 288255 - 289
112THRTHRVALVALBB42 - 10343 - 104
212THRTHRVALVALFF42 - 10343 - 104
322GLUGLULEULEUBB108 - 149109 - 150
422GLUGLULEULEUFF108 - 149109 - 150
532PHEPHEPROPROBB160 - 195161 - 196
632PHEPHEPROPROFF160 - 195161 - 196
742LEULEUPROPROBB207 - 243208 - 244
842LEULEUPROPROFF207 - 243208 - 244
952CYSCYSASPASPBB254 - 288255 - 289
1052CYSCYSASPASPFF254 - 288255 - 289
113THRTHRVALVALCC42 - 10343 - 104
213THRTHRVALVALGG42 - 10343 - 104
323GLUGLULEULEUCC108 - 149109 - 150
423GLUGLULEULEUGG108 - 149109 - 150
533PHEPHEPROPROCC160 - 195161 - 196
633PHEPHEPROPROGG160 - 195161 - 196
743LEULEUPROPROCC207 - 243208 - 244
843LEULEUPROPROGG207 - 243208 - 244
953CYSCYSASPASPCC254 - 288255 - 289
1053CYSCYSASPASPGG254 - 288255 - 289
114THRTHRVALVALDD42 - 10343 - 104
214THRTHRVALVALHH42 - 10343 - 104
324GLUGLULEULEUDD108 - 149109 - 150
424GLUGLULEULEUHH108 - 149109 - 150
534PHEPHEPROPRODD160 - 195161 - 196
634PHEPHEPROPROHH160 - 195161 - 196
744LEULEUPROPRODD207 - 243208 - 244
844LEULEUPROPROHH207 - 243208 - 244
954CYSCYSASPASPDD254 - 288255 - 289
1054CYSCYSASPASPHH254 - 288255 - 289

NCS ensembles :
ID
1
2
3
4
DetailsThe biological unit is a tetramer. There are 2 biological units in the asymmetric unit (chains A, B, C & D and chains E, F, G & H).

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Components

#1: Protein
U4/U6 snRNA-associated splicing factor PRP24 / U4/U6 snRNP protein


Mass: 33401.359 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast)
Gene: PRP24 / Plasmid: PVP 16 / Production host: Escherichia coli (E. coli) / Strain (production host): B834 P(RARE2) / References: UniProt: P49960
#2: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 193 / Source method: isolated from a natural source / Formula: H2O
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.65 Å3/Da / Density % sol: 53.61 %
Crystal growTemperature: 277 K / Method: vapor diffusion, hanging drop
Details: PROTEIN SOLUTION (10 MG/ML PROTEIN, 0.050 M SODIUM CHLORIDE, 0.0003 M TCEP, 0.005 TRIS PH 8.0) MIXED IN A 1:1 RATIO WITH THE WELL SOLUTION (14% MEPEG 5000, 0.20 M TETRAMETHYL AMMONIUM ...Details: PROTEIN SOLUTION (10 MG/ML PROTEIN, 0.050 M SODIUM CHLORIDE, 0.0003 M TCEP, 0.005 TRIS PH 8.0) MIXED IN A 1:1 RATIO WITH THE WELL SOLUTION (14% MEPEG 5000, 0.20 M TETRAMETHYL AMMONIUM CHLORIDE, 0.10 M SODIUM SUCCINATE PH 4.0) Crystals cryo-protected with the well solution supplemented with 30% ethylene glycol., VAPOR DIFFUSION, HANGING DROP, temperature 277K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 0.97911 Å
DetectorType: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Oct 11, 2005
Details: HORIZONTAL SAGITALLY FOCUSING 2ND BENT MONOCHROMATOR CRYSTAL, VERTICAL BENT FOCUSING MIRROR
RadiationMonochromator: CRYOGENICALLY COOLED SI (220) DOUBLE BOUNCE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97911 Å / Relative weight: 1
ReflectionResolution: 2.7→49.503 Å / Num. obs: 74236 / % possible obs: 94.2 % / Redundancy: 7 % / Rmerge(I) obs: 0.122 / Χ2: 1.105 / Net I/σ(I): 7.416
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. unique allΧ2% possible all
2.7-2.85.40.4892.3958010.97774.3
2.8-2.9160.39661950.99380.2
2.91-3.046.30.33269991.02689.6
3.04-3.26.70.27276311.11697.9
3.2-3.47.30.22278221.163100
3.4-3.667.60.15978061.158100
3.66-4.037.60.12178681.163100
4.03-4.627.60.0978981.119100
4.62-5.827.50.09279661.093100
5.82-49.57.20.08482501.11599.7

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Phasing

PhasingMethod: MAD
Phasing MAD set

R cullis centric: 0 / Highest resolution: 4 Å / Lowest resolution: 48.1 Å / Power centric: 0

IDR cullis acentricPower acentricReflection acentricReflection centric
ISO_100217012960
ANO_10.6821.653217000
Phasing MAD set shell

R cullis centric: 0 / Power centric: 0

IDResolution (Å)R cullis acentricPower acentricReflection acentricReflection centric
ISO_116.82-48.100229139
ISO_112.27-16.8200414147
ISO_110.13-12.2700554148
ISO_18.82-10.1300662148
ISO_17.92-8.8200753142
ISO_17.24-7.9200838150
ISO_16.72-7.2400919147
ISO_16.29-6.7200981148
ISO_15.94-6.29001060149
ISO_15.64-5.94001123155
ISO_15.38-5.64001183149
ISO_15.15-5.38001237145
ISO_14.95-5.15001294145
ISO_14.77-4.95001352148
ISO_14.61-4.77001397150
ISO_14.47-4.61001445147
ISO_14.34-4.47001502152
ISO_14.21-4.34001542143
ISO_14.1-4.21001588148
ISO_14-4.1001628160
ANO_116.82-48.10.7781.3922280
ANO_112.27-16.820.6591.8044140
ANO_110.13-12.270.671.7935540
ANO_18.82-10.130.6561.7786620
ANO_17.92-8.820.5812.1647530
ANO_17.24-7.920.6042.0728380
ANO_16.72-7.240.5712.2099190
ANO_16.29-6.720.5662.2749810
ANO_15.94-6.290.6172.04310600
ANO_15.64-5.940.5662.18611230
ANO_15.38-5.640.6271.94111830
ANO_15.15-5.380.661.70712370
ANO_14.95-5.150.71.54512940
ANO_14.77-4.950.7351.49613520
ANO_14.61-4.770.7621.32813970
ANO_14.47-4.610.7291.32414450
ANO_14.34-4.470.7711.21115020
ANO_14.21-4.340.7541.29215420
ANO_14.1-4.210.7811.16515880
ANO_14-4.10.8221.09116280
Phasing MAD set site
IDCartn x (Å)Cartn y (Å)Cartn z (Å)Atom type symbolB isoOccupancy
1-5.231-109.79-2.42SE58.012.33
215.908-156.67114.473SE56.772.45
341.072-144.7810.255SE42.492.12
4-17.616-133.706-6.427SE74.352.53
5-8.409-146.278-9.385SE61.12.33
620.208-105.249.339SE61.582.25
7-12.816-93.45914.576SE61.562.44
8-7.318-137.722-12.667SE62.42.21
99.005-73.9246.55SE59.892.25
1010.999-117.7886.392SE56.832.25
11-33.734-141.4982.568SE68.632.3
1210.111-112.583-6.362SE86.922.43
1312.397-81.71710.288SE52.852.1
14-13.918-90.38723.324SE71.512.35
1521.289-113.82112.682SE73.012.26
16-30.474-139.18610.687SE79.612.54
17-11.499-92.575-4.013SE55.261.85
18-2.8-76.9889.649SE93.452.96
19-40.024-158.7454.162SE57.931.88
2025.159-146.18622.015SE61.181.99
2137.667-137.1786.514SE68.792.3
2247.45-155.213-3.921SE45.471.85
23-18.407-138.8456.373SE87.132.46
2425.9-140.2269.726SE92.233.04
2515.212-94.46623.49SE64.81.88
26-3.55-82.79822.005SE75.082.05
2714.819-153.6823.271SE59.752.11
2818.702-92.046-3.906SE45.081.81
29-13.407-157.042-23.6SE52.71.79
30-7.968-111.47116.043SE65.531.93
3120.797-174.80515.982SE63.561.91
32-1.901-112.296-10.575SE85.92.61
Phasing dmFOM : 0.53 / FOM acentric: 0.53 / FOM centric: 0.49 / Reflection: 74149 / Reflection acentric: 67967 / Reflection centric: 6182
Phasing dm shell
Resolution (Å)FOM FOM acentricFOM centricReflectionReflection acentricReflection centric
7.7-48.0990.750.780.6235962828768
4.8-7.70.720.740.571064493901254
3.9-4.80.730.750.6213191120351156
3.4-3.90.630.640.51324012247993
2.9-3.40.390.40.3422330209381392
2.7-2.90.190.190.211114810529619

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Processing

Software
NameVersionClassificationNB
DENZOdata reduction
SCALEPACKdata scaling
SHARPphasing
RESOLVE2.1phasing
REFMAC5.2.0005refinement
PDB_EXTRACT1.701data extraction
PHENIXphasing
SHELXDphasing
ARP/wARPmodel building
RefinementMethod to determine structure: SAD / Resolution: 2.7→49.503 Å / Cor.coef. Fo:Fc: 0.952 / Cor.coef. Fo:Fc free: 0.924 / WRfactor Rfree: 0.27 / WRfactor Rwork: 0.218 / SU B: 26.293 / SU ML: 0.255 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / ESU R: 0.754 / ESU R Free: 0.344
Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. THE ELECTRON DENSITY MAP INDICATES SOME DISORDER IN THE RRM1 DOMAIN (Residues 41 to 117) OF CHAINS D and H.
RfactorNum. reflection% reflectionSelection details
Rfree0.2642 3738 5.041 %random
Rwork0.213 ---
all0.216 ---
obs-74155 94.218 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL PLUS MASK
Displacement parametersBiso mean: 56.783 Å2
Baniso -1Baniso -2Baniso -3
1--2.223 Å20 Å20 Å2
2--5.883 Å20 Å2
3----3.66 Å2
Refinement stepCycle: LAST / Resolution: 2.7→49.503 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms15026 0 0 193 15219
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0120.02215262
X-RAY DIFFRACTIONr_angle_refined_deg1.381.96820562
X-RAY DIFFRACTIONr_dihedral_angle_1_deg5.96651840
X-RAY DIFFRACTIONr_dihedral_angle_2_deg34.72323.9718
X-RAY DIFFRACTIONr_dihedral_angle_3_deg20.165152860
X-RAY DIFFRACTIONr_dihedral_angle_4_deg19.81715122
X-RAY DIFFRACTIONr_chiral_restr0.0960.22354
X-RAY DIFFRACTIONr_gen_planes_refined0.0040.0211280
X-RAY DIFFRACTIONr_nbd_refined0.2130.26413
X-RAY DIFFRACTIONr_nbtor_refined0.3120.210393
X-RAY DIFFRACTIONr_xyhbond_nbd_refined0.1380.2525
X-RAY DIFFRACTIONr_symmetry_vdw_refined0.2380.249
X-RAY DIFFRACTIONr_symmetry_hbond_refined0.1570.24
X-RAY DIFFRACTIONr_mcbond_it0.88429539
X-RAY DIFFRACTIONr_mcangle_it1.681415096
X-RAY DIFFRACTIONr_scbond_it3.11266314
X-RAY DIFFRACTIONr_scangle_it4.85185466
Refine LS restraints NCS

Dom-ID: 1 / Refine-ID: X-RAY DIFFRACTION

Ens-IDAuth asym-IDNumberTypeRms dev position (Å)Weight position
1A848tight positional0.0320.05
1A857medium positional0.2760.5
1A848tight thermal0.0830.5
1A857medium thermal0.632
2B848tight positional0.030.05
2B857medium positional0.2210.5
2B848tight thermal0.0760.5
2B857medium thermal0.6412
3C848tight positional0.0310.05
3C857medium positional0.2390.5
3C848tight thermal0.0540.5
3C857medium thermal0.5872
4D848tight positional0.0310.05
4D857medium positional0.3190.5
4D848tight thermal0.0580.5
4D857medium thermal0.5752
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 20

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRfactor allNum. reflection all% reflection obs (%)
2.7-2.770.4082090.35139980.354573673.344
2.77-2.8460.3352310.31541630.316563278.018
2.846-2.9280.3191990.28442730.285544482.145
2.928-3.0180.3362230.28145080.284528689.501
3.018-3.1170.3112410.27547020.277513996.186
3.117-3.2260.3012580.24846880.251498299.277
3.226-3.3480.2572410.22445690.226481199.979
3.348-3.4850.2672370.21643940.219463299.978
3.485-3.6390.2622410.20542230.2084464100
3.639-3.8170.2442390.20840310.214270100
3.817-4.0230.2522060.19538360.1984042100
4.023-4.2670.2461780.18936750.1913853100
4.267-4.5610.2281990.16734260.173625100
4.561-4.9250.1791800.15632110.1573391100
4.925-5.3940.2351530.18529710.1883124100
5.394-6.0290.2821440.20627190.2092863100
6.029-6.9580.3041290.20823850.2122514100
6.958-8.5130.2621040.19820660.2012170100
8.513-120.232790.18216270.1841706100
12-1060.348470.2679520.271102297.75
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.57461.39721.58475.23591.20917.60770.2125-0.3446-0.51030.2285-0.0082-0.42890.58660.4223-0.2043-0.35480.0373-0.05310.04280.0916-0.3149-6.3109-38.316527.4299
24.27360.5241-0.28282.67380.40255.346-0.0412-0.2049-0.2652-0.0999-0.1595-0.1215-0.05710.54480.2007-0.21320.04920.0048-0.12940.032-0.1426-11.0639-33.84745.7342
34.49590.0732-0.65164.8861-0.3736.6218-0.04510.2257-0.6406-0.53170.01060.17180.8793-0.08270.03450.0068-0.0293-0.0432-0.3568-0.02880.0589-23.063-49.7818-8.9902
43.1358-0.1511-0.68663.4906-0.71569.1978-0.00690.5049-0.17870.0025-0.0345-0.3273-0.27520.7740.0415-0.2881-0.02260.01860.0818-0.0892-0.3778-12.6765-27.5605-47.2101
53.90650.43470.69891.24840.56844.4266-0.01450.3655-0.0353-0.1039-0.0287-0.08710.3460.48970.0431-0.083-0.01180.0128-0.0962-0.0596-0.1806-12.8715-29.9292-24.8006
65.28810.38391.25443.029-1.0827.10630.0993-0.1310.58130.1257-0.06240.0128-0.44390.2792-0.0369-0.0832-0.0920.0333-0.4535-0.0801-0.0358-10.4777-11.5426-9.2465
75.86372.8366-0.75798.6515-1.01865.58170.3301-1.7276-0.62020.9256-0.40920.34490.33640.15370.0791-0.2081-0.00710.03820.31160.3058-0.0627-43.9499-46.809523.4992
83.45811.3127-1.85063.3467-0.43231.57150.0107-0.09560.0885-0.12730.17760.36660.0024-0.7681-0.1883-0.13810.0416-0.05090.05250.02720.0046-38.7692-34.21245.5928
95.0837-0.91150.4514.869-0.81095.4978-0.2023-0.41230.6560.23110.14080.0394-0.99650.06510.06140.01110.0714-0.0105-0.1912-0.16060.0043-26.7845-12.6377.7099
1010.3446-0.77364.11868.2571-0.83911.41490.18881.17931.619-1.4168-0.07480.1129-1.1285-1.1678-0.1140.52750.3468-0.05520.10480.41870.2756-36.76171.4709-34.3218
114.4325-0.42421.8334.1792-1.13344.3081-0.13730.21110.45060.10310.43780.2687-0.6965-0.8808-0.30050.00880.1414-0.0182-0.12570.05870.029-35.4237-13.5622-17.7644
126.12030.9897-0.87223.4342-2.46356.19980.01380.4701-0.6192-0.1720.1550.03580.5884-0.2209-0.1688-0.1599-0.0592-0.0942-0.1273-0.0731-0.0976-39.2911-37.8676-21.1465
134.33391.21830.31185.08511.04978.61940.132-0.3897-0.49110.22070.0127-0.41680.63790.5285-0.1447-0.34270.0966-0.0660.05960.0804-0.3235-35.0781-101.497527.4346
144.12190.19770.23792.78460.42317.0052-0.0613-0.153-0.2367-0.1692-0.1941-0.1071-0.05020.66790.2553-0.20220.09160.0113-0.05620.0287-0.1553-39.8185-97.06125.7914
155.0028-0.0680.36344.8311-0.64958.9916-0.0240.2974-0.7873-0.44900.131.04940.11230.024-0.00740.08070.0233-0.3415-0.10820.0676-51.8262-113.0009-9.0191
163.7514-0.2604-0.45123.8444-1.02929.38-0.07510.4565-0.1531-0.0015-0.0897-0.3346-0.40121.08010.1649-0.3047-0.07370.03760.2019-0.0987-0.3871-41.3943-90.8198-47.2406
174.2150.34670.58360.84291.06474.8822-0.02220.4265-0.0711-0.054-0.0889-0.0710.19710.91340.1111-0.1196-0.01320.01350.083-0.0439-0.1519-41.5787-93.159-24.8434
185.29410.88511.26013.6377-2.04686.3605-0.0594-0.11520.64650.2327-0.02760.0383-0.72110.54230.08710.0126-0.1257-0.0409-0.1531-0.0452-0.0382-39.2279-74.7481-9.2546
195.74683.3859-1.3258.8163-0.53526.68580.4581-1.8652-0.74561.033-0.66060.13720.35040.00190.2024-0.1857-0.0130.04560.43620.286-0.0676-72.7738-110.081723.411
203.07970.8553-1.39652.661-0.00331.10870.001-0.12660.0259-0.02580.08520.3313-0.0114-0.5967-0.0862-0.11830.0948-0.02680.0651-0.0104-0.0018-67.5528-97.53645.5062
214.9943-0.97510.50555.2689-1.20437.5522-0.2095-0.6170.66320.1652-0.07550.162-1.35910.04420.2850.10780.1383-0.0825-0.2541-0.2015-0.0153-55.5479-75.97347.5765
227.031-0.78533.139810.5366-2.834712.0430.00051.32521.3571-1.27790.2040.2083-1.3681-0.7972-0.20440.63010.1589-0.20510.23760.41460.2543-65.4767-61.9214-34.4588
234.93080.49281.71134.41230.04115.3179-0.23880.23310.61580.08490.21550.1946-0.8195-0.62930.02330.05380.1648-0.0781-0.2533-0.01740.0029-64.1573-76.9519-17.8633
245.30540.92410.56463.3493-2.04426.1392-0.03470.2784-0.635-0.14730.0815-0.00350.5798-0.1344-0.0468-0.18430.0229-0.025-0.154-0.1242-0.0962-68.0483-101.2049-21.2064
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION / Selection: ALL

IDRefine TLS-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11AA41 - 11742 - 118
22AA118 - 196119 - 197
33AA206 - 291207 - 292
44BB41 - 11742 - 118
55BB118 - 196119 - 197
66BB206 - 291207 - 292
77CC41 - 11742 - 118
88CC118 - 196119 - 197
99CC206 - 291207 - 292
1010DD41 - 11742 - 118
1111DD118 - 196119 - 197
1212DD206 - 291207 - 292
1313EE41 - 11742 - 118
1414EE118 - 196119 - 197
1515EE206 - 291207 - 292
1616FF41 - 11742 - 118
1717FF118 - 196119 - 197
1818FF206 - 291207 - 292
1919GG41 - 11742 - 118
2020GG118 - 196119 - 197
2121GG206 - 291207 - 292
2222HH41 - 11742 - 118
2323HH118 - 196119 - 197
2424HH206 - 291207 - 292

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