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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 2gd3 | ||||||
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タイトル | NMR structure of S14G-humanin in 30% TFE solution | ||||||
![]() | Humanin | ||||||
![]() | UNKNOWN FUNCTION / S14G-Humanin / Humanin / Alzheimer's disease / Neuroprotection / NMR / CD | ||||||
機能・相同性 | ![]() negative regulation of interleukin-18 production / receptor antagonist activity / negative regulation of neuroinflammatory response / negative regulation of interleukin-1 production / negative regulation of NLRP3 inflammasome complex assembly / negative regulation of execution phase of apoptosis / Formyl peptide receptors bind formyl peptides and many other ligands / negative regulation of amyloid fibril formation / leukocyte chemotaxis / negative regulation of response to oxidative stress ...negative regulation of interleukin-18 production / receptor antagonist activity / negative regulation of neuroinflammatory response / negative regulation of interleukin-1 production / negative regulation of NLRP3 inflammasome complex assembly / negative regulation of execution phase of apoptosis / Formyl peptide receptors bind formyl peptides and many other ligands / negative regulation of amyloid fibril formation / leukocyte chemotaxis / negative regulation of response to oxidative stress / sperm flagellum / supramolecular fiber organization / sperm midpiece / mitochondrion organization / G protein-coupled receptor binding / negative regulation of inflammatory response / cellular response to amyloid-beta / : / cell-cell signaling / G alpha (i) signalling events / G alpha (q) signalling events / intracellular iron ion homeostasis / negative regulation of neuron apoptotic process / signaling receptor binding / apoptotic process / negative regulation of apoptotic process / perinuclear region of cytoplasm / mitochondrion / extracellular space / extracellular region / identical protein binding / nucleus / cytoplasm 類似検索 - 分子機能 | ||||||
手法 | 溶液NMR / simulated annealing torsion angle dynamics | ||||||
![]() | Benaki, D. / Zikos, C. / Evangelou, A. / Livaniou, E. / Vlassi, M. / Mikros, E. / Pelecanou, M. | ||||||
![]() | ![]() タイトル: Solution structure of Ser14Gly-humanin, a potent rescue factor against neuronal cell death in Alzheimer's disease. 著者: Benaki, D. / Zikos, C. / Evangelou, A. / Livaniou, E. / Vlassi, M. / Mikros, E. / Pelecanou, M. #1: ![]() タイトル: Solution structure of humanin, a peptide against Alzheimer's disease-related neurotoxicity. 著者: Benaki, D. / Zikos, C. / Evangelou, A. / Livaniou, E. / Vlassi, M. / Mikros, E. / Pelecanou, M. #2: ジャーナル: Proc.Natl.Acad.Sci.Usa / 年: 2001 タイトル: A rescue factor abolishing neuronal cell death by a wide spectrum of familial Alzheimer's disease genes and Abeta. 著者: Hashimoto, Y. / Niikura, T. / Tajima, H. / Yasukawa, T. / Sudo, H. / Ito, Y. / Kita, Y. / Kawasumi, M. / Kouyama, K. / Doyu, M. / Sobue, G. / Koide, T. / Tsuji, S. / Lang, J. / Kurokawa, K. / Nishimoto, I. #3: ![]() タイトル: Mechanisms of Neuroprotection by a Novel Rescue Factor Humanin from Swedish Mutant Amyloid Precursor Protein 著者: Hashimoto, Y. / Ito, Y. / Niikura, T. / Shao, Z. / Hata, M. / Oyama, F. / Nishimoto, I. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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PDB形式 | ![]() | 75.9 KB | 表示 | ![]() |
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その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 342 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 411.4 KB | 表示 | |
XML形式データ | ![]() | 7.1 KB | 表示 | |
CIF形式データ | ![]() | 10.4 KB | 表示 | |
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-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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NMR アンサンブル |
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要素
#1: タンパク質・ペプチド | 分子量: 2661.239 Da / 分子数: 1 / 変異: S14G / 由来タイプ: 合成 詳細: S14G-humanin was prepared by Fmoc-solid phase synthesis on o-Cl-trityl-amidomethyl polystyrene resin (Evangelou, A., Zikos, C., Livaniou, E., Evangelatos, G.P. "Highyield, solid-phase ...詳細: S14G-humanin was prepared by Fmoc-solid phase synthesis on o-Cl-trityl-amidomethyl polystyrene resin (Evangelou, A., Zikos, C., Livaniou, E., Evangelatos, G.P. "Highyield, solid-phase synthesis of humanin, an Alzheimer's disease associated, novel 24-mer peptide which contains a difficult sequence" J. Peptide Sci. 2004, 10, 631 635). The peptide was purified to 95% with semi-preparative RP-HPLC and suitably characterized. The sequence is naturally found in Homo sapiens (human). 参照: UniProt: Q8IVG9 |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||
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NMR実験 |
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NMR実験の詳細 | Text: This structure was determined using standard 2D homonuclear techniques |
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試料調製
詳細 | 内容: 1.3 mM S14G-humanin; 60 microM NaN3 to prevent microbial growth; H2O/TFE-d3 7:3; pH 2.6 (uncorrected for the presence of TFE); at 298 and 280 K 溶媒系: H2O/TFE-d3 7:3 | |||||||||||||||
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試料状態 |
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-NMR測定
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M |
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放射波長 | 相対比: 1 |
NMRスペクトロメーター | タイプ: Bruker AVANCE / 製造業者: Bruker / モデル: AVANCE / 磁場強度: 500 MHz |
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解析
NMR software |
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精密化 | 手法: simulated annealing torsion angle dynamics / ソフトェア番号: 1 詳細: Starting from an extended structure a total of 300 structures were generated from 133 NOE-derived distance restraints and 2 distance restraints based on temperature coefficient data using the ...詳細: Starting from an extended structure a total of 300 structures were generated from 133 NOE-derived distance restraints and 2 distance restraints based on temperature coefficient data using the simulated annealing and energy minimization protocol in the program CNS, version 1.1 | ||||||||||||||||||||||||
代表構造 | 選択基準: lowest energy | ||||||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: 14 convergent conformers are presented having the lowest energy and the best structural quality in Ramachadran plot 計算したコンフォーマーの数: 300 / 登録したコンフォーマーの数: 14 |