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- PDB-2g6u: Solution structures of MP-2: a high affinity miniprotein ligand t... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2g6u | ||||||
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Title | Solution structures of MP-2: a high affinity miniprotein ligand to Streptavidin | ||||||
![]() | Miniprotein MP2 | ||||||
![]() | DE NOVO PROTEIN / miniprotein / type-1 beta turn / high affinity peptide to streptavidin | ||||||
Method | SOLUTION NMR / distance geometry, simulated annealing | ||||||
![]() | Fox, R.O. / Mukherjee, M. | ||||||
![]() | ![]() Title: Structural studies of high affinity miniprotein ligands to Streptavidin Authors: Luo, J. / Mukherjee, M. / Fan, X. / Yang, H.W. / Liu, D. / Khan, R. / White, M. / Fox, R.O. | ||||||
History |
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Remark 999 | SEQUENCE THERE IS NO SUITABLE SEQUENCE DATABASE REFERENCE AT THE TIME OF PROCESSING. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 121 KB | Display | ![]() |
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PDB format | ![]() | 91.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 341.2 KB | Display | ![]() |
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Full document | ![]() | 496.3 KB | Display | |
Data in XML | ![]() | 7.6 KB | Display | |
Data in CIF | ![]() | 11.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 1783.150 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: This sequence was identified to be a high affinity streptavidin binding ligand by phage display |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear experiments |
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Sample preparation
Details | Contents: 1.2mM MP-2 / Solvent system: 20mM Sod acetate; 90% H2O, 10% D2O |
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Sample conditions | pH: 5.3 / Pressure: ambient / Temperature: 283 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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Processing
NMR software | Name: CNS / Version: 1.1 / Developer: Alex Brunger / Classification: refinement |
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Refinement | Method: distance geometry, simulated annealing / Software ordinal: 1 |
NMR representative | Selection criteria: lowest energy |
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 30 |