[English] 日本語
Yorodumi- PDB-2g6u: Solution structures of MP-2: a high affinity miniprotein ligand t... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2g6u | ||||||
---|---|---|---|---|---|---|---|
Title | Solution structures of MP-2: a high affinity miniprotein ligand to Streptavidin | ||||||
Components | Miniprotein MP2 | ||||||
Keywords | DE NOVO PROTEIN / miniprotein / type-1 beta turn / high affinity peptide to streptavidin | ||||||
Method | SOLUTION NMR / distance geometry, simulated annealing | ||||||
Authors | Fox, R.O. / Mukherjee, M. | ||||||
Citation | Journal: To be Published Title: Structural studies of high affinity miniprotein ligands to Streptavidin Authors: Luo, J. / Mukherjee, M. / Fan, X. / Yang, H.W. / Liu, D. / Khan, R. / White, M. / Fox, R.O. | ||||||
History |
| ||||||
Remark 999 | SEQUENCE THERE IS NO SUITABLE SEQUENCE DATABASE REFERENCE AT THE TIME OF PROCESSING. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2g6u.cif.gz | 121 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2g6u.ent.gz | 91.7 KB | Display | PDB format |
PDBx/mmJSON format | 2g6u.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/g6/2g6u ftp://data.pdbj.org/pub/pdb/validation_reports/g6/2g6u | HTTPS FTP |
---|
-Related structure data
Related structure data | |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein/peptide | Mass: 1783.150 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: This sequence was identified to be a high affinity streptavidin binding ligand by phage display |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
| ||||||||||||||||
NMR details | Text: This structure was determined using standard 2D homonuclear experiments |
-Sample preparation
Details | Contents: 1.2mM MP-2 / Solvent system: 20mM Sod acetate; 90% H2O, 10% D2O |
---|---|
Sample conditions | pH: 5.3 / Pressure: ambient / Temperature: 283 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
|
-Processing
NMR software | Name: CNS / Version: 1.1 / Developer: Alex Brunger / Classification: refinement |
---|---|
Refinement | Method: distance geometry, simulated annealing / Software ordinal: 1 |
NMR representative | Selection criteria: lowest energy |
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 30 |