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Yorodumi- PDB-2g46: structure of vSET in complex with meK27 H3 Pept. and cofactor pro... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2g46 | ||||||
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Title | structure of vSET in complex with meK27 H3 Pept. and cofactor product SAH | ||||||
Components |
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Keywords | TRANSFERASE / vSET structure / hsitone methyltransferase | ||||||
Function / homology | Function and homology information histone H3K37 methyltransferase activity / nucleosome / methylation / protein heterodimerization activity / DNA binding / identical protein binding Similarity search - Function | ||||||
Biological species | Paramecium bursaria Chlorella virus 1 | ||||||
Method | SOLUTION NMR / simulated annealing torsion angle dynamics | ||||||
Authors | Qian, C.M. / Zheng, L. / Zhou, M.M. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2006 Title: Structural insights of the specificity and catalysis of a viral histone H3 lysine 27 methyltransferase. Authors: Qian, C.M. / Wang, X. / Manzur, K. / Sachchidanand / Farooq, A. / Zeng, L. / Wang, R. / Zhou, M.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2g46.cif.gz | 1.7 MB | Display | PDBx/mmCIF format |
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PDB format | pdb2g46.ent.gz | 1.5 MB | Display | PDB format |
PDBx/mmJSON format | 2g46.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2g46_validation.pdf.gz | 523 KB | Display | wwPDB validaton report |
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Full document | 2g46_full_validation.pdf.gz | 1 MB | Display | |
Data in XML | 2g46_validation.xml.gz | 164 KB | Display | |
Data in CIF | 2g46_validation.cif.gz | 182.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/g4/2g46 ftp://data.pdbj.org/pub/pdb/validation_reports/g4/2g46 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13606.623 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Paramecium bursaria Chlorella virus 1 / Genus: Chlorovirus / Gene: A612L / Plasmid: PET22B / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: GenBank: 9632146, UniProt: O41094*PLUS #2: Protein/peptide | Mass: 2127.513 Da / Num. of mol.: 2 / Source method: obtained synthetically / References: UniProt: O24165*PLUS #3: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy. |
-Sample preparation
Details | Contents: 0.5 mM protein with 2.5 mM meK27 H3 Pept. and 2.5mM SAH Solvent system: in a 50 mM phosphate buffer of pH 6.5 containing 700 mM NaCl, 300 mM urea, 0.1 mM EDTA and 5 mM -ME in H2O/2H2O (9/1) or 2H2O |
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Sample conditions | Ionic strength: 300mM NaCl and 700mM Urea / pH: 6.5 / Pressure: 1 atm / Temperature: 310 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | ||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |