[English] 日本語
![](img/lk-miru.gif)
- PDB-2fow: THE RNA BINDING DOMAIN OF RIBOSOMAL PROTEIN L11: THREE-DIMENSIONA... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 2fow | ||||||
---|---|---|---|---|---|---|---|
Title | THE RNA BINDING DOMAIN OF RIBOSOMAL PROTEIN L11: THREE-DIMENSIONAL STRUCTURE OF THE RNA-BOUND FORM OF THE PROTEIN, NMR, 26 STRUCTURES | ||||||
![]() | RIBOSOMAL PROTEIN L11 | ||||||
![]() | RIBOSOME / PROTEIN:RNA / THIOSTREPTON | ||||||
Function / homology | ![]() large ribosomal subunit rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / DNA binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / SUBSTRUCTURE DISTANCE GEOMETRY, SIMULATING ANNEALING | ||||||
![]() | Hinck, A.P. / Markus, M.A. / Huang, S. / Grzesiek, S. / Kustanovich, I. / Draper, D.E. / Torchia, D.A. | ||||||
![]() | ![]() Title: The RNA binding domain of ribosomal protein L11: three-dimensional structure of the RNA-bound form of the protein and its interaction with 23 S rRNA. Authors: Hinck, A.P. / Markus, M.A. / Huang, S. / Grzesiek, S. / Kustonovich, I. / Draper, D.E. / Torchia, D.A. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 587.4 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 491.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-
Components
#1: Protein | Mass: 8152.554 Da / Num. of mol.: 1 / Fragment: C-TERMINAL DOMAIN, 75 RESIDUES / Mutation: F1M Source method: isolated from a genetically manipulated source Details: STRUCTURE OF THE PROTEIN IN THIS ENTRY WAS DETERMINED AS A 1\:1 COMPLEX WITH ITS TARGET RNA OF 58 NUCLEOTIDES, BASES 1051 - 1108 OF THE E. COLI SEQUENCE Source: (gene. exp.) ![]() ![]() Cell line: BL21 / Plasmid: BL21 / Species (production host): Escherichia coli / Production host: ![]() ![]() |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
---|
-
Sample preparation
Sample conditions | pH: 6.1 / Temperature: 320 K |
---|---|
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
|
---|
-
Processing
Software |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR software |
| ||||||||||||
Refinement | Method: SUBSTRUCTURE DISTANCE GEOMETRY, SIMULATING ANNEALING Software ordinal: 1 Details: SUBSTRUCTURE DISTANCE GEOMETRY/ SIMULATED ANNEALING PROTOCOL | ||||||||||||
NMR ensemble | Conformer selection criteria: NOE VIOL < 0.3 ANGSTROMS DIHEDRAL VIOL < 3 DEGREES; 3JHNHA COUPLING VIOLATED < 1.5 HZ Conformers calculated total number: 50 / Conformers submitted total number: 26 |