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Yorodumi- PDB-2f90: Crystal structure of bisphosphoglycerate mutase in complex with 3... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2f90 | ||||||
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Title | Crystal structure of bisphosphoglycerate mutase in complex with 3-phosphoglycerate and AlF4- | ||||||
Components | Bisphosphoglycerate mutase | ||||||
Keywords | ISOMERASE / bisphosphoglycerate mutase | ||||||
Function / homology | Function and homology information carbohydrate derivative catabolic process / bisphosphoglycerate mutase / bisphosphoglycerate mutase activity / 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity / phosphoglycerate mutase (2,3-diphosphoglycerate-dependent) / neuroinflammatory response / establishment of blood-brain barrier / respiratory gaseous exchange by respiratory system / Glycolysis / erythrocyte development ...carbohydrate derivative catabolic process / bisphosphoglycerate mutase / bisphosphoglycerate mutase activity / 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity / phosphoglycerate mutase (2,3-diphosphoglycerate-dependent) / neuroinflammatory response / establishment of blood-brain barrier / respiratory gaseous exchange by respiratory system / Glycolysis / erythrocyte development / defense response to protozoan / oxygen transport / glycolytic process / hydrolase activity / carbohydrate metabolic process / extracellular exosome / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Wang, Y. / Liu, L. / Wei, Z. / Gong, W. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2006 Title: Seeing the process of histidine phosphorylation in human bisphosphoglycerate mutase Authors: Wang, Y. / Liu, L. / Wei, Z. / Cheng, Z. / Lin, Y. / Gong, W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2f90.cif.gz | 128.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2f90.ent.gz | 100 KB | Display | PDB format |
PDBx/mmJSON format | 2f90.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f9/2f90 ftp://data.pdbj.org/pub/pdb/validation_reports/f9/2f90 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | The dimer is the biological units |
-Components
#1: Protein | Mass: 31118.375 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: pET22b / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) References: UniProt: P07738, bisphosphoglycerate mutase, EC: 5.4.2.1, EC: 3.1.3.13 #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.21 Å3/Da / Density % sol: 44.31 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 7.1 Details: 25% PEG6000, 0.1M Hepes 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
-Data collection
Diffraction | Mean temperature: 93 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU / Wavelength: 1.5418 Å |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Sep 4, 2005 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→50 Å / Num. obs: 37619 / % possible obs: 98.4 % / Redundancy: 6.6 % / Biso Wilson estimate: 18.5 Å2 / Rmerge(I) obs: 0.075 / Rsym value: 0.075 / Net I/σ(I): 24.1 |
Reflection shell | Resolution: 2→2.05 Å / Redundancy: 6.5 % / Rmerge(I) obs: 0.298 / Mean I/σ(I) obs: 6.2 / Num. unique all: 2459 / Rsym value: 0.298 / % possible all: 98.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2→30 Å / Rfactor Rfree error: 0.004 / Data cutoff high absF: 334689.7 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 62.8018 Å2 / ksol: 0.397086 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 21.7 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2→30 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.13 Å / Rfactor Rfree error: 0.01 / Total num. of bins used: 6
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Xplor file |
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