+Open data
-Basic information
Entry | Database: PDB / ID: 2erc | ||||||
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Title | CRYSTAL STRUCTURE OF ERMC' A RRNA-METHYL TRANSFERASE | ||||||
Components | RRNA METHYL TRANSFERASE | ||||||
Keywords | METHYLTRANSFERASE / ANTIBIOTIC RESISTANCE / RRNA METHYLTRANSFERASE / ERYTHROMYCIN RESISTANCE METHYLASE C' | ||||||
Function / homology | Function and homology information 23S rRNA (adenine2085-N6)-dimethyltransferase / 23S rRNA (adenine(2085)-N(6))-dimethyltransferase activity / rRNA (adenine-N6,N6-)-dimethyltransferase activity / response to antibiotic / RNA binding Similarity search - Function | ||||||
Biological species | Bacillus subtilis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD PHASING IN A DIFFERENT EXPERIMENT / Resolution: 3.03 Å | ||||||
Authors | Bussiere, D.E. / Muchmore, S.W. / Dealwis, C.G. / Schluckebier, G. / Abad-Zapatero, C. | ||||||
Citation | Journal: Biochemistry / Year: 1998 Title: Crystal structure of ErmC', an rRNA methyltransferase which mediates antibiotic resistance in bacteria. Authors: Bussiere, D.E. / Muchmore, S.W. / Dealwis, C.G. / Schluckebier, G. / Nienaber, V.L. / Edalji, R.P. / Walter, K.A. / Ladror, U.S. / Holzman, T.F. / Abad-Zapatero, C. #1: Journal: J.Bacteriol. / Year: 1995 Title: Substrate Requirements for Ermc' Methyltransferase Activity Authors: Zhong, P. / Pratt, S.D. / Edalji, R.P. / Walter, K.A. / Holzman, T.F. / Shivakumar, A.G. / Katz, L. #2: Journal: J.Bacteriol. / Year: 1986 Title: Sequence and Properties of Pim13, a Macrolide-Lincosamide-Streptogramin B Resistance Plasmid from Bacillus Subtilis Authors: Monod, M. / Denoya, C. / Dubnau, D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2erc.cif.gz | 107 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2erc.ent.gz | 84.5 KB | Display | PDB format |
PDBx/mmJSON format | 2erc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/er/2erc ftp://data.pdbj.org/pub/pdb/validation_reports/er/2erc | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.4984, 0.8669, -0.0024), Vector: |
-Components
#1: Protein | Mass: 28955.529 Da / Num. of mol.: 2 / Fragment: RESIDUES 10-244 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus subtilis (bacteria) / Strain: BD1109 / Cell line: B834 / Gene: ERMC' / Variant: ERMC' / Plasmid: PTERM31 / Gene (production host): ERMC' / Production host: Escherichia coli (E. coli) / Strain (production host): BL219 (DE3) PLYSS / References: UniProt: P13956, EC: 2.1.1.48 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.1 Å3/Da / Density % sol: 55 % Description: DATA FOR MAD PHASING WAS DONE WITH OTHER EXPERIMENTAL CONDITIONS. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 4 / Details: pH 4.0 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.5 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 103 K |
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Diffraction source | Source: SYNCHROTRON / Site: CHESS / Beamline: F1 / Wavelength: 0.92178 |
Detector | Type: TATE ET AL. (1995) J. APPL. CRYST. 28, 196-205. / Detector: CCD / Date: Oct 1, 1996 / Details: MIRROR |
Radiation | Monochromator: SI(111) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92178 Å / Relative weight: 1 |
Reflection | Highest resolution: 3 Å / Num. obs: 14568 / % possible obs: 94 % / Observed criterion σ(I): 1 / Redundancy: 9.5 % / Rmerge(I) obs: 0.068 / Rsym value: 0.12 / Net I/σ(I): 10 |
Reflection shell | Resolution: 3→3.5 Å / Redundancy: 6 % / Rmerge(I) obs: 0.027 / Mean I/σ(I) obs: 2 / Rsym value: 0.03 / % possible all: 84.3 |
Reflection | *PLUS Num. measured all: 138382 |
Reflection shell | *PLUS % possible obs: 84.3 % |
-Processing
Software |
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Refinement | Method to determine structure: MAD PHASING IN A DIFFERENT EXPERIMENT Resolution: 3.03→8 Å / Rfactor Rfree error: 0.01 / Data cutoff high absF: 10000000000000 / Data cutoff low absF: 0.0001 / Cross valid method: THROUGHOUT / σ(F): 0 Details: STRUCTURE WAS SOLVED IN P622 SPACE GROUP BUT WAS REFINED IN P6.
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Displacement parameters | Biso mean: 67.4 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.03→8 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: RESTRAINTS / Rms dev Biso : 0.05 Å2 / Rms dev position: 0.04 Å / Weight Biso : 2 / Weight position: 200 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 3.03→3.13 Å / Rfactor Rfree error: 0.049 / Total num. of bins used: 10
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Xplor file |
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Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor obs: 0.313 |