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- PDB-2eqq: Solution structure of growth-blocking peptide of the armyworm, Ps... -

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Basic information

Entry
Database: PDB / ID: 2eqq
TitleSolution structure of growth-blocking peptide of the armyworm, Pseudaletia separata
ComponentsGrowth-blocking peptide, long form
KeywordsCYTOKINE / GROWTH-BLOCKING PEPTIDE
Function / homologyParalytic/GBP/PSP peptide / Paralytic/GBP/PSP peptide / hemocyte migration / regulation of synaptic transmission, dopaminergic / positive regulation of cell division / cytokine activity / growth factor activity / extracellular space / Growth-blocking peptide, long form
Function and homology information
Biological speciesMythimna separata (northern armyworm)
MethodSOLUTION NMR
AuthorsUmetsu, Y. / Aizawa, T. / Kamiya, M. / Kumaki, Y. / Demura, M. / Kawano, K.
CitationJournal: J.Biol.Chem. / Year: 2009
Title: C-terminal elongation of growth-blocking peptide enhances its biological activity and micelle binding affinity
Authors: Umetsu, Y. / Aizawa, T. / Muto, K. / Yamamoto, H. / Kamiya, M. / Kumaki, Y. / Mizuguchi, M. / Demura, M. / Hayakawa, Y. / Kawano, K.
History
DepositionMar 30, 2007Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Apr 1, 2008Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2011Group: Version format compliance

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Growth-blocking peptide, long form


Theoretical massNumber of molelcules
Total (without water)3,1161
Polymers3,1161
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 100structures with the lowest energy
RepresentativeModel #1lowest energy

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Components

#1: Protein/peptide Growth-blocking peptide, long form / GBP


Mass: 3115.542 Da / Num. of mol.: 1 / Fragment: Growth-blocking peptide, long form
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mythimna separata (northern armyworm) / Production host: Escherichia coli (E. coli) / References: UniProt: Q27913

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
111DQF-COSY
1212D NOESY
1312D TOCSY

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Sample preparation

DetailsContents: 3mM 1-28GBP(pH4.4); 90% H2O, 10% D2O / Solvent system: 90% H2O/10% D2O
Sample conditionsIonic strength: 0 / pH: 4.4 / Pressure: ambient / Temperature: 283 K

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NMR measurement

NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Bruker DRXBrukerDRX5001
JEOL ECAJEOLECA6002

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Processing

NMR software
NameVersionClassification
CNS1.1structure solution
CNS1.1refinement
NMR representativeSelection criteria: lowest energy
NMR ensembleConformer selection criteria: structures with the lowest energy
Conformers calculated total number: 100 / Conformers submitted total number: 20

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