+Open data
-Basic information
Entry | Database: PDB / ID: 2e1a | ||||||
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Title | crystal structure of FFRP-DM1 | ||||||
Components | 75aa long hypothetical regulatory protein AsnC | ||||||
Keywords | TRANSCRIPTION / transcriptional regulatory protein / archaea | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Pyrococcus horikoshii (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å | ||||||
Authors | Koike, H. / Suzuki, M. | ||||||
Citation | Journal: Structure / Year: 2007 Title: A Structural Code for Discriminating between Transcription Signals Revealed by the Feast/Famine Regulatory Protein DM1 in Complex with Ligands Authors: Okamura, H. / Yokoyama, K. / Koike, H. / Yamada, M. / Shimowasa, A. / Kabasawa, M. / Kawashima, T. / Suzuki, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2e1a.cif.gz | 74.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2e1a.ent.gz | 57.6 KB | Display | PDB format |
PDBx/mmJSON format | 2e1a.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2e1a_validation.pdf.gz | 457.3 KB | Display | wwPDB validaton report |
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Full document | 2e1a_full_validation.pdf.gz | 462.7 KB | Display | |
Data in XML | 2e1a_validation.xml.gz | 16.8 KB | Display | |
Data in CIF | 2e1a_validation.cif.gz | 23.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/e1/2e1a ftp://data.pdbj.org/pub/pdb/validation_reports/e1/2e1a | HTTPS FTP |
-Related structure data
Related structure data | 2z4pSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 8611.233 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pyrococcus horikoshii (archaea) / Strain: OT3 / Plasmid: pET3a / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: O73983 #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.66 Å3/Da / Density % sol: 66.43 % Description: THE STRUCTURE FACTOR FILE CONTAINS FRIEDEL PAIRS. |
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Crystal grow | Temperature: 278 K / Method: vapor diffusion, sitting drop / pH: 4 Details: 1.0M LiCl, 10% PEG 6000, 100mM citrate, 20% glycerol, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Photon Factory / Beamline: BL-18B / Wavelength: 0.97925 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jan 20, 2002 |
Radiation | Monochromator: Si / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97925 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→81 Å / Num. obs: 35886 / % possible obs: 100 % / Observed criterion σ(I): 2 / Redundancy: 5.6 % / Biso Wilson estimate: 27.6 Å2 / Rmerge(I) obs: 0.092 / Net I/σ(I): 7.7 |
Reflection shell | Resolution: 2.5→2.64 Å / Redundancy: 5.5 % / Rmerge(I) obs: 0.299 / Mean I/σ(I) obs: 2.5 / % possible all: 99.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2Z4P Resolution: 2.5→81 Å / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / Stereochemistry target values: Engh & Huber / Details: THE STRUCTURE FACTOR FILE CONTAINS FRIEDEL PAIRS.
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.5→81 Å
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Refine LS restraints |
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