+Open data
-Basic information
Entry | Database: PDB / ID: 2crm | ||||||
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Title | Solution structure of the forth FNIII domain of human | ||||||
Components | Fibronectin type-III domain containing protein 3a | ||||||
Keywords | CELL ADHESION / FIBRONECTIN TYPE III DOMAIN / Structural Genomics / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information Sertoli cell development / vesicle membrane / fertilization / spermatid development / acrosomal vesicle / cell-cell adhesion / Golgi membrane / intracellular membrane-bounded organelle / Golgi apparatus / RNA binding ...Sertoli cell development / vesicle membrane / fertilization / spermatid development / acrosomal vesicle / cell-cell adhesion / Golgi membrane / intracellular membrane-bounded organelle / Golgi apparatus / RNA binding / membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Izumi, K. / Hayashi, F. / Yoshida, M. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be published Title: Solution structure of the forth FNIII domain of human Authors: Izumi, K. / Hayashi, F. / Yoshida, M. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2crm.cif.gz | 682.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2crm.ent.gz | 592.2 KB | Display | PDB format |
PDBx/mmJSON format | 2crm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cr/2crm ftp://data.pdbj.org/pub/pdb/validation_reports/cr/2crm | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12734.272 Da / Num. of mol.: 1 / Fragment: fn3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Description: cell free protein synthesis / Gene: FNDC3, KIAA0970 / Plasmid: P040628-02 / References: UniProt: Q9Y2H6 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.79mM 13C, 15N-labeled protein; 20mM d-Tris-HCl; 100mM NaCl; 1mM DTT; 0.02% NaN3; 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 120mM / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: JEOL ECA / Manufacturer: JEOL / Model: ECA / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function, structures with the lowest energy, structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 |