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Yorodumi- PDB-2bxx: Crystal structure of the N-terminal domain of IBV coronavirus nuc... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2bxx | ||||||
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Title | Crystal structure of the N-terminal domain of IBV coronavirus nucleocapsid. Native crystal form | ||||||
Components | NUCLEOCAPSID PROTEINVirus | ||||||
Keywords | NUCLEOCAPSID PROTEIN / PHOSPHORYLATION / RNA-BINDING / VIRAL NUCLEOPROTEIN | ||||||
Function / homology | Function and homology information host cell endoplasmic reticulum-Golgi intermediate compartment / host cell Golgi apparatus / viral nucleocapsid / ribonucleoprotein complex / RNA binding / identical protein binding Similarity search - Function | ||||||
Biological species | AVIAN INFECTIOUS BRONCHITIS VIRUS | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.85 Å | ||||||
Authors | Fan, H. / Ooi, A. / Liu, D.-X. / Lescar, J. | ||||||
Citation | Journal: Structure / Year: 2005 Title: The Nucleocapsid Protein of Coronavirus Infectious Bronchitis Virus: Crystal Structure of its N-Terminal Domain and Multimerization Properties. Authors: Fan, H. / Ooi, A. / Tan, Y.W. / Wang, S. / Fang, S. / Liu, D.-X. / Lescar, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2bxx.cif.gz | 67.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2bxx.ent.gz | 50 KB | Display | PDB format |
PDBx/mmJSON format | 2bxx.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bx/2bxx ftp://data.pdbj.org/pub/pdb/validation_reports/bx/2bxx | HTTPS FTP |
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-Related structure data
Related structure data | 2btlSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.376, -0.927, 0.003), Vector: |
-Components
#1: Protein | Mass: 15014.549 Da / Num. of mol.: 2 / Fragment: RNA BINDING DOMAIN RESIDUES 29-160 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) AVIAN INFECTIOUS BRONCHITIS VIRUS / Strain: BEAUDETTE / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: P69597, UniProt: P69596*PLUS #2: Water | ChemComp-HOH / | Compound details | ENGINEERED | Sequence details | ENGINEERED | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.02 Å3/Da / Density % sol: 38.62 % |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU MICROMAX-007 / Wavelength: 1.5418 |
Detector | Detector: IMAGE PLATE / Date: Dec 10, 2004 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.85→20 Å / Num. obs: 20031 / % possible obs: 92.4 % / Observed criterion σ(I): 0 / Redundancy: 3.8 % / Rmerge(I) obs: 0.06 / Net I/σ(I): 7.4 |
Reflection shell | Resolution: 1.85→1.95 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.62 / Mean I/σ(I) obs: 1.1 / % possible all: 88.8 |
-Processing
Software | Name: CNS / Version: 1.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2BTL Resolution: 1.85→19.92 Å / Cross valid method: THROUGHOUT / Stereochemistry target values: MAXIMUM LIKEIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 34.284 Å2
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Refinement step | Cycle: LAST / Resolution: 1.85→19.92 Å
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Refine LS restraints |
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