SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED.
RESIDUES ARE NUMBERED TO CORRELATE WITH RESIDUE NUMBERING OF 3,4-PCD FROM PSEUDOMONAS PUTIDA ...RESIDUES ARE NUMBERED TO CORRELATE WITH RESIDUE NUMBERING OF 3,4-PCD FROM PSEUDOMONAS PUTIDA STRUCTURE, AND SO DO NOT EXACTLY CORRELATE WITH WHAT ONE WOULD EXPECT FOR A SEQUENTIAL NUMBERING. THIS INCLUDES AN INSERTION OF 5 RESIDUES IN THE A SUBUNIT BETWEEN K88 AND F89.
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.61 Å3/Da / 溶媒含有率: 52.3 %
結晶化
pH: 7.5 詳細: 1.8 M AMMONIUM SULFATE, 100 MM TRIS-MALEATE PH 7.5, 0.08% PEG 4000, PROTEIN AT 20 MG/ML
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データ収集
回折
平均測定温度: 194 K
放射光源
由来: 回転陽極 / タイプ: RIGAKU RU200B / 波長: 1.5418
検出器
タイプ: RIGAKU R-AXIS IV / 詳細: OSMIC CONFOCAL MAXFLUX OPTICS
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1.5418 Å / 相対比: 1
反射
解像度: 1.8→20 Å / Num. obs: 46943 / % possible obs: 99.5 % / Observed criterion σ(I): 0 / 冗長度: 3.5 % / Rmerge(I) obs: 0.06 / Net I/σ(I): 12
反射 シェル
解像度: 1.8→1.85 Å / 冗長度: 3.4 % / Rmerge(I) obs: 0.34 / Mean I/σ(I) obs: 4.4 / % possible all: 99.3