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- PDB-2bsw: Crystal structure of a glyphosate-N-acetyltransferase obtained by... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2bsw | ||||||
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Title | Crystal structure of a glyphosate-N-acetyltransferase obtained by DNA shuffling. | ||||||
![]() | GLYPHOSATE N-ACETYLTRANSFERASE | ||||||
![]() | TRANSFERASE / GNAT SUPERFAMILY / GLYPHOSATE N-ACETYLTRANSFERASE | ||||||
Function / homology | ![]() acyltransferase activity, transferring groups other than amino-acyl groups Similarity search - Function | ||||||
Biological species | SYNTHETIC CONSTRUCT (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Keenan, R.J. / Siehl, D.L. / Gorton, R. / Castle, L.A. | ||||||
![]() | ![]() Title: DNA Shuffling as a Tool for Protein Crystallization. Authors: Keenan, R.J. / Siehl, D.L. / Gorton, R. / Castle, L.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 45.1 KB | Display | ![]() |
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PDB format | ![]() | 35.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 736.4 KB | Display | ![]() |
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Full document | ![]() | 738.6 KB | Display | |
Data in XML | ![]() | 10.4 KB | Display | |
Data in CIF | ![]() | 14.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 16812.572 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) SYNTHETIC CONSTRUCT (others) Description: THE ENZYME IS A SHUFFLED VARIANT DERIVED FROM GENES DISCOVERED IN B. LICHENIFORMIS. Plasmid: PQE80 / Production host: ![]() ![]() | ||
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#2: Chemical | ChemComp-CAO / | ||
#3: Chemical | ChemComp-SO4 / | ||
#4: Chemical | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.57 % |
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Crystal grow | pH: 4.6 Details: 100 MM SODIUM ACETATE, PH 4.6 250 MM AMMONIUM SULFATE 25% PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Dec 12, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97942 Å / Relative weight: 1 |
Reflection | Resolution: 1.63→50 Å / Num. obs: 18939 / % possible obs: 97.4 % / Observed criterion σ(I): 0 / Redundancy: 2.94 % / Rmerge(I) obs: 0.07 / Net I/σ(I): 20.2 |
Reflection shell | Resolution: 1.63→1.69 Å / Rmerge(I) obs: 0.16 / Mean I/σ(I) obs: 8 / % possible all: 98.1 |
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Processing
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Refinement | Method to determine structure: ![]() Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. THE N-TERMINAL SELENOMETHIONINE, MSE A1, IS DISORDERED IN ELECTRON DENSITY MAPS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 18.65 Å2
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Refinement step | Cycle: LAST / Resolution: 1.63→45.17 Å
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Refine LS restraints |
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