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- PDB-2bvv: SUGAR RING DISTORTION IN THE GLYCOSYL-ENZYME INTERMEDIATE OF A FA... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2bvv | ||||||
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Title | SUGAR RING DISTORTION IN THE GLYCOSYL-ENZYME INTERMEDIATE OF A FAMILY G/11 XYLANASE. | ||||||
![]() | PROTEIN (ENDO-1,4-BETA-XYLANASE) | ||||||
![]() | HYDROLASE / XYLANASE / GLYCOSIDASE | ||||||
Function / homology | ![]() endo-1,4-beta-xylanase activity / endo-1,4-beta-xylanase / xylan catabolic process Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Sidhu, G. / Brayer, G.D. | ||||||
![]() | ![]() Title: Sugar ring distortion in the glycosyl-enzyme intermediate of a family G/11 xylanase. Authors: Sidhu, G. / Withers, S.G. / Nguyen, N.T. / McIntosh, L.P. / Ziser, L. / Brayer, G.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 49.1 KB | Display | ![]() |
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PDB format | ![]() | 35.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 363.3 KB | Display | ![]() |
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Full document | ![]() | 364.4 KB | Display | |
Data in XML | ![]() | 5.1 KB | Display | |
Data in CIF | ![]() | 7.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 20393.006 Da / Num. of mol.: 1 / Mutation: Y69F Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 44.91 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.5 / Details: pH 7.5 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→10 Å / Num. obs: 30126 / % possible obs: 92.7 % / Redundancy: 4.3 % / Rmerge(I) obs: 0.064 / Net I/σ(I): 16.2 |
Reflection | *PLUS Num. measured all: 128221 |
Reflection shell | *PLUS Highest resolution: 1.5 Å / Lowest resolution: 1.55 Å / Rmerge(I) obs: 0.199 / Mean I/σ(I) obs: 3.6 |
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Processing
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Refinement | Method to determine structure: OTHER / Resolution: 1.5→10 Å / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 1.5→10 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.188 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |