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- PDB-2bsf: Structure of the C-terminal receptor-binding domain of avian reov... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2bsf | ||||||
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Title | Structure of the C-terminal receptor-binding domain of avian reovirus fibre sigmaC, Zn crystal form. | ||||||
![]() | SIGMA C CAPSID PROTEIN | ||||||
![]() | VIRAL PROTEIN / ORTHOREOVIRUS / TRIPLE BETA-SPIRAL / BETA-BARREL | ||||||
Function / homology | ![]() viral capsid / symbiont entry into host cell / virion attachment to host cell Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Guardado Calvo, P. / Fox, G.C. / Hermo Parrado, X.L. / Llamas-Saiz, A.L. / van Raaij, M.J. | ||||||
![]() | ![]() Title: Structure of the Carboxy-Terminal Receptor-Binding Domain of Avian Reovirus Fibre Sigmac Authors: Guardado Calvo, P. / Fox, G.C. / Hermo Parrado, X.L. / Llamas-Saiz, A.L. / Costas, C. / Martinez-Costas, J. / Benavente, J. / van Raaij, M.J. #1: Journal: Acta Crystallogr.,Sect.F / Year: 2005 Title: Crystallisation of the C-Terminal Globular Domain of Avian Reovirus Fibre Authors: van Raaij, M.J. / Hermo Parrado, X.L. / Guardado Calvo, P. / Fox, G.C. / Llamas-Saiz, A.L. / Costas, C. / Martinez-Costas, J. / Benavente, J. #2: ![]() Title: Crystal Structure of Reovirus Attachment Protein Sigma1 Reveals Evolutionary Relationship to Adenovirus Fiber Authors: Chappell, J.D. / Prota, A.E. / Dermody, T.S. / Stehle, T. #3: Journal: J.Virol. / Year: 1997 Title: Protein Architecture of Avian Reovirus S1133 and Identification of the Cell Attachment Protein Authors: Martinez-Costas, J. / Grande, A. / Varela, R. / Garcia-Martinez, C. / Benavente, J. #4: Journal: Virology / Year: 2000 Title: Oligomerization and Cell-Binding Properties of the Avian Reovirus Cell-Attachment Protein Sigmac Authors: Grande, A. / Rodriguez, E. / Costas, C. / Everitt, E. / Benavente, J. #5: Journal: Virology / Year: 2001 Title: The Avian Reovirus Genome Segment S1 is a Functionally Tricistronic Gene that Expresses One Structure and Two Nonstructural Proteins in Infected Cells Authors: Bodelon, G. / Labrada, L. / Martinez-Costas, J. / Benavente, J. #6: Journal: J.Gen.Virol. / Year: 2002 Title: Subunit Composition and Conformational Stability of the Oligomeric Form of the Avian Reovirus Cell-Attachment Protein Sigmac Authors: Grande, A. / Costas, C. / Benavente, J. | ||||||
History |
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Remark 700 | SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 51.6 KB | Display | ![]() |
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PDB format | ![]() | 36.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 439.6 KB | Display | ![]() |
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Full document | ![]() | 439.8 KB | Display | |
Data in XML | ![]() | 10.6 KB | Display | |
Data in CIF | ![]() | 14.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 18912.947 Da / Num. of mol.: 1 Fragment: C-TERMINAL RECEPTOR-BINDING REGION, RESIDUES 151-326 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Description: THE AVIAN REOVIRUS STRAIN S1133 WAS ORIGINALLY PROVIDED BY DR. PHILIP I.MARCUS WHEN DR. J. BENAVENTE WAS A ROCHE VISITING SCIENTIST IN THE LABORATORY OF DR. A.SHATKIN Plasmid: PET28CPLUS / Production host: ![]() ![]() | ||||
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#2: Chemical | #3: Chemical | ChemComp-ZN / | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.2 Å3/Da / Density % sol: 0.61 % |
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Crystal grow | pH: 8.5 Details: 100 MM TRIS-HCL PH 8.5, 1.5 M AMMONIUM SULPHATE, 12% GLYCEROL, 10 MM ZINC SULPHATE |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||
Detector | Type: MARRESEARCH / Detector: CCD / Date: Dec 17, 2004 / Details: TOROIDAL MIRROR | |||||||||||||||
Radiation | Monochromator: DOUBLE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.1→30 Å / Num. obs: 14231 / % possible obs: 98.7 % / Observed criterion σ(I): 0 / Redundancy: 7.8 % / Biso Wilson estimate: 28.27 Å2 / Rmerge(I) obs: 0.07 / Net I/σ(I): 7.3 | |||||||||||||||
Reflection shell | Resolution: 2.1→2.21 Å / Redundancy: 8 % / Rmerge(I) obs: 0.29 / Mean I/σ(I) obs: 2.6 / % possible all: 97.7 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 25.93 Å2
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Refinement step | Cycle: LAST / Resolution: 2.1→74.54 Å
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Refine LS restraints |
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