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Yorodumi- PDB-2bpr: NMR STRUCTURE OF THE SUBSTRATE BINDING DOMAIN OF DNAK, 25 STRUCTURES -
+Open data
-Basic information
Entry | Database: PDB / ID: 2bpr | ||||||
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Title | NMR STRUCTURE OF THE SUBSTRATE BINDING DOMAIN OF DNAK, 25 STRUCTURES | ||||||
Components | DNAK | ||||||
Keywords | MOLECULAR CHAPERONE / HSP70 / PEPTIDE BINDING / PROTEIN FOLDING | ||||||
Function / homology | Function and homology information stress response to copper ion / sigma factor antagonist activity / chaperone cofactor-dependent protein refolding / protein unfolding / cellular response to unfolded protein / inclusion body / protein folding chaperone / heat shock protein binding / ATP-dependent protein folding chaperone / ADP binding ...stress response to copper ion / sigma factor antagonist activity / chaperone cofactor-dependent protein refolding / protein unfolding / cellular response to unfolded protein / inclusion body / protein folding chaperone / heat shock protein binding / ATP-dependent protein folding chaperone / ADP binding / unfolded protein binding / protein-folding chaperone binding / protein refolding / response to heat / DNA replication / protein-containing complex assembly / ATP hydrolysis activity / protein-containing complex / zinc ion binding / ATP binding / membrane / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | SOLUTION NMR / DISTANCE GEOMETRY, RESTRAINED MOLECULAR DYNAMICS WITH SIMULATED ANNEALING | ||||||
Authors | Wang, H. / Kurochkin, A.V. / Pang, Y. / Hu, W. / Flynn, G.C. / Zuiderweg, E.R.P. | ||||||
Citation | Journal: Biochemistry / Year: 1998 Title: NMR solution structure of the 21 kDa chaperone protein DnaK substrate binding domain: a preview of chaperone-protein interaction. Authors: Wang, H. / Kurochkin, A.V. / Pang, Y. / Hu, W. / Flynn, G.C. / Zuiderweg, E.R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2bpr.cif.gz | 1.3 MB | Display | PDBx/mmCIF format |
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PDB format | pdb2bpr.ent.gz | 1 MB | Display | PDB format |
PDBx/mmJSON format | 2bpr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2bpr_validation.pdf.gz | 363.1 KB | Display | wwPDB validaton report |
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Full document | 2bpr_full_validation.pdf.gz | 566.6 KB | Display | |
Data in XML | 2bpr_validation.xml.gz | 89 KB | Display | |
Data in CIF | 2bpr_validation.cif.gz | 116.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bp/2bpr ftp://data.pdbj.org/pub/pdb/validation_reports/bp/2bpr | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 20940.344 Da / Num. of mol.: 1 / Fragment: SUBSTRATE BINDING DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: XL-1 BLUE / Plasmid: PQE30 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A6Y8 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: H2O AND D2O |
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Sample conditions | Ionic strength: 50 mM INORGANIC PHOSPHATE / pH: 7 / Pressure: 1 atm / Temperature: 298 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: DISTANCE GEOMETRY, RESTRAINED MOLECULAR DYNAMICS WITH SIMULATED ANNEALING Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE REFERENCE CITED ABOVE | |||||||||
NMR ensemble | Conformer selection criteria: TOTAL ENERGY / Conformers calculated total number: 100 / Conformers submitted total number: 25 |