[English] 日本語
Yorodumi- PDB-2blm: BETA-LACTAMASE OF BACILLUS LICHENIFORMIS 749(SLASH)C AT 2 ANGSTRO... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2blm | ||||||
---|---|---|---|---|---|---|---|
Title | BETA-LACTAMASE OF BACILLUS LICHENIFORMIS 749(SLASH)C AT 2 ANGSTROMS RESOLUTION | ||||||
Components | BETA-LACTAMASE | ||||||
Keywords | HYDROLASE(ACTING IN CYCLIC AMIDES) | ||||||
Function / homology | Function and homology information beta-lactam antibiotic catabolic process / beta-lactamase activity / beta-lactamase / response to antibiotic / plasma membrane Similarity search - Function | ||||||
Biological species | Bacillus licheniformis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2 Å | ||||||
Authors | Moews, P.C. / Knox, J.R. / Dideberg, O. | ||||||
Citation | Journal: Proteins / Year: 1990 Title: Beta-lactamase of Bacillus licheniformis 749/C at 2 A resolution. Authors: Moews, P.C. / Knox, J.R. / Dideberg, O. / Charlier, P. / Frere, J.M. #1: Journal: Molecular Recognition. Chemical and Biochemical Problems Year: 1989 Title: Crystallographic Comparison of Penicillin-Recognizing Enzymes Authors: Knox, J.R. / Kelly, J.A. #2: Journal: Science / Year: 1986 Title: On the Origin of Bacterial Resistance to Penicillin. Comparison of a Beta-Lactamase and a Penicillin Target Authors: Kelly, J.A. / Dideberg, O. / Charlier, P. / Wery, J.P. / Libert, M. / Moews, P.C. / Knox, J.R. / Duez, C. / Fraipont, C. / Joris, B. / Dusart, J. / Frere, J.M. / Ghuysen, J.M. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2blm.cif.gz | 27.9 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2blm.ent.gz | 14.3 KB | Display | PDB format |
PDBx/mmJSON format | 2blm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2blm_validation.pdf.gz | 315 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 2blm_full_validation.pdf.gz | 315 KB | Display | |
Data in XML | 2blm_validation.xml.gz | 857 B | Display | |
Data in CIF | 2blm_validation.cif.gz | 5.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bl/2blm ftp://data.pdbj.org/pub/pdb/validation_reports/bl/2blm | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
2 |
| ||||||||
Unit cell |
| ||||||||
Atom site foot note | 1: RESIDUES PRO A 167 AND PRO B 167 ARE CIS PROLINES. | ||||||||
Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.8708, 0.00104, 0.49164), Vector: |
-Components
#1: Protein | Mass: 29542.436 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus licheniformis (bacteria) / References: UniProt: P00808, beta-lactamase |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
---|
-Sample preparation
Crystal | Density Matthews: 2.16 Å3/Da / Density % sol: 43.15 % | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | *PLUS pH: 5.5 / Method: vapor diffusion / Details: used macroseeding | |||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Reflection | *PLUS Highest resolution: 2 Å / Lowest resolution: 6 Å / Num. measured all: 53000 / Rmerge(I) obs: 0.053 |
---|
-Processing
Software | Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 2→10 Å / σ(F): 3 /
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→10 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.208 / Rfactor Rwork: 0.219 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |