Mass: 18.015 Da / Num. of mol.: 290 / Source method: isolated from a natural source / Formula: H2O
Sequence details
THE VARIANT DESCRIBED IN THE SEQADV RECORDS BELOW HAS NOT BEEN ANNOTATED IN THE UNIPROT SEQUENCE ...THE VARIANT DESCRIBED IN THE SEQADV RECORDS BELOW HAS NOT BEEN ANNOTATED IN THE UNIPROT SEQUENCE DATABASE ENTRY P11309. 261 SER IS PHOSPHORYLATED
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.7 Å3/Da / Density % sol: 67 %
Crystal grow
Temperature: 277 K / Method: vapor diffusion, sitting drop Details: 0.2 M NA2SO4, 0.1 M BIS-TRIS-PROPANE PH 7.5, 20% PEG3350, 10% ETHYLENE-GLYCOL, 0.5% DMSO, SITTING DROP, VAPOR DIFFUSION, 277 K
Resolution: 1.8→86.07 Å / Cor.coef. Fo:Fc: 0.97 / Cor.coef. Fo:Fc free: 0.961 / SU B: 4.029 / SU ML: 0.057 / Cross valid method: THROUGHOUT / ESU R: 0.115 / ESU R Free: 0.086 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.183
2128
5.1 %
RANDOM
Rwork
0.157
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obs
0.159
39487
99.9 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK