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Yorodumi- PDB-2bbz: Crystal Structure of MC159 Reveals Molecular Mechanism of DISC As... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2bbz | ||||||
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Title | Crystal Structure of MC159 Reveals Molecular Mechanism of DISC Assembly and vFLIP Inhibition | ||||||
Components | Viral CASP8 and FADD-like apoptosis regulator | ||||||
Keywords | VIRAL PROTEIN / death effector domain | ||||||
Function / homology | Function and homology information symbiont-mediated suppression of host NF-kappaB cascade / symbiont-mediated perturbation of host apoptosis / symbiont-mediated suppression of host apoptosis / Caspase activation via Death Receptors in the presence of ligand / suppression by virus of host autophagy / positive regulation of necroptotic process / negative regulation of apoptotic signaling pathway / host cell cytoplasm / host cell nucleus / apoptotic process Similarity search - Function | ||||||
Biological species | Molluscum contagiosum virus subtype 1 | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 3.8 Å | ||||||
Authors | Yang, J.K. / Wang, L. / Zheng, L. / Wan, F. / Ahmed, M. / Lenardo, M.J. / Wu, H. | ||||||
Citation | Journal: Mol.Cell / Year: 2005 Title: Crystal structure of MC159 reveals molecular mechanism of DISC assembly and FLIP inhibition. Authors: Yang, J.K. / Wang, L. / Zheng, L. / Wan, F. / Ahmed, M. / Lenardo, M.J. / Wu, H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2bbz.cif.gz | 144.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2bbz.ent.gz | 116.8 KB | Display | PDB format |
PDBx/mmJSON format | 2bbz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bb/2bbz ftp://data.pdbj.org/pub/pdb/validation_reports/bb/2bbz | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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4 |
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Unit cell |
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-Components
#1: Protein | Mass: 28038.932 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Molluscum contagiosum virus subtype 1 / Genus: Molluscipoxvirus / Species: Molluscum contagiosum virus / Strain: subtype 1 / Plasmid: modified pET28b / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) Codon Plus RIPL / References: UniProt: Q98325 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.88 Å3/Da / Density % sol: 57.29 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 5 Details: 50-150 mM sodium/potassium phosphate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 31-ID / Wavelength: 0.9795 Å |
Detector | Detector: CCD / Date: Sep 24, 2004 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 3.8→30 Å / Num. all: 13309 / Num. obs: 13309 / % possible obs: 99.7 % / Observed criterion σ(I): -3 |
Reflection shell | Resolution: 3.8→3.94 Å / % possible all: 99.2 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 3.8→30 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 3.8→30 Å
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Refine LS restraints |
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