Mass: 8063.382 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Flock house virus / Genus: Alphanodavirus / Gene: B2 / Plasmid: pETM-11 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P68831
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
3D 15N-separated NOESY
1
2
2
3D 13C-separated NOESY
1
3
1
HNHA
NMR details
Text: Distance restraints were derived from 15N- or 13C-resolved 3D NOESY. HN-N residual dipolar couplings were measured using a spin-state-selective 1H,15N correlation experiment in dilute liquid ...Text: Distance restraints were derived from 15N- or 13C-resolved 3D NOESY. HN-N residual dipolar couplings were measured using a spin-state-selective 1H,15N correlation experiment in dilute liquid crystalline medium. Restraints for the backbone angles phi and psi were derived from TALOS. Stereospecific assignments of Leu, Val methyl groups were obtained using a 10% fractionally 13C-labelled sample.
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
0.2 - 1 mM 15N, 13C/15N or 2H/13C/15N labelled B2 protein in 50 mM sodium phosphate pH 6.3, 50 mM sodium chloride, 1 mM DTT, 90% H2O / 10% D2O
90% H2O/10% D2O
2
0.2 - 1 mM 13C/15N labelled B2 protein in 50 mM sodium phosphate pH 6.3, 50 mM sodium chloride, 1 mM DTT, 100% D2O
100% D2O
Sample conditions
Ionic strength: 50 mM NaPi, 50 mM NaCl / pH: 6.3 / Pressure: 1 atm / Temperature: 295 K
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NMR measurement
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
Radiation wavelength
Relative weight: 1
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Bruker DRX
Bruker
DRX
500
1
Bruker DRX
Bruker
DRX
600
2
Bruker DRX
Bruker
DRX
900
3
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Processing
NMR software
Name
Version
Developer
Classification
XwinNMR
3.5
collection
NMRPipe
Delaglioetal.
processing
NMRView
5
Johnsonetal.
dataanalysis
TALOS
Cornilescuetal.
dataanalysis
ARIA
1.2
Lingeetal.
structuresolution
CNS
1.1
Brungeretal.
structuresolution
CNS
1.1
Brungeretal.
refinement
Refinement
Method: molecular dynamics, simulated annealing / Software ordinal: 1 Details: The experimentally determined distance, dihedral and dipolar coupling restraints were applied in a simulated annealing protocol using ARIA and CNS. Non-crystallographic symmetry (NCS) ...Details: The experimentally determined distance, dihedral and dipolar coupling restraints were applied in a simulated annealing protocol using ARIA and CNS. Non-crystallographic symmetry (NCS) restraints were used to enforce the dimer symmetry. The final ensemble of NMR structures was refined in a shell of water molecules.
NMR representative
Selection criteria: lowest energy
NMR ensemble
Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10
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