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Yorodumi- PDB-2aja: X-Ray structure of an ankyrin repeat family protein Q5ZSV0 from L... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2aja | ||||||
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Title | X-Ray structure of an ankyrin repeat family protein Q5ZSV0 from Legionella pneumophila. Northeast Structural Genomics Consortium target LgR21. | ||||||
Components | ankyrin repeat family protein | ||||||
Keywords | PROTEIN BINDING / NESG / Q5ZSV0 / Structural Genomics / PSI / Protein Structure Initiative / Northeast Structural Genomics Consortium | ||||||
Function / homology | Ankyrin repeat-containing domain / Ankyrin repeat-containing domain superfamily / Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat / Alpha Horseshoe / Mainly Alpha / Ankyrin repeat family protein Function and homology information | ||||||
Biological species | Legionella pneumophila (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.8 Å | ||||||
Authors | Kuzin, A.P. / Chen, Y. / Acton, T. / Xiao, R. / Conover, K. / Ma, C. / Kellie, R. / Montelione, G.T. / Tong, L. / Hunt, J.F. / Northeast Structural Genomics Consortium (NESG) | ||||||
Citation | Journal: To be Published Title: X-Ray structure of an ankyrin repeat family protein Q5ZSV0 from Legionella pneumophila. Authors: Kuzin, A.P. / Chen, Y. / Acton, T. / Xiao, R. / Conover, K. / Ma, C. / Kellie, R. / Montelione, G.T. / Tong, L. / Hunt, J.F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2aja.cif.gz | 142.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2aja.ent.gz | 119.1 KB | Display | PDB format |
PDBx/mmJSON format | 2aja.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2aja_validation.pdf.gz | 442.3 KB | Display | wwPDB validaton report |
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Full document | 2aja_full_validation.pdf.gz | 469.4 KB | Display | |
Data in XML | 2aja_validation.xml.gz | 28.7 KB | Display | |
Data in CIF | 2aja_validation.cif.gz | 39.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/aj/2aja ftp://data.pdbj.org/pub/pdb/validation_reports/aj/2aja | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 43265.336 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Legionella pneumophila (bacteria) / Plasmid: pET21 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) + Magic / References: UniProt: Q5ZSV0 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 51 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 0.2M potassium thiocyanate, 20% PEG 3350, 20 mM EDTA, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X4A / Wavelength: 0.979 / Wavelength: 0.979 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jul 12, 2005 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→30 Å / Num. all: 40286 / % possible obs: 99.9 % / Observed criterion σ(I): -3 / Redundancy: 4.95 % / Biso Wilson estimate: 28.2 Å2 / Rsym value: 0.07 |
Reflection shell | Resolution: 2.8→2.9 Å / Rsym value: 0.235 / % possible all: 99.9 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 2.8→19.92 Å / Rfactor Rfree error: 0.007 / Data cutoff high absF: 287667.08 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 13.9618 Å2 / ksol: 0.27268 e/Å3 | ||||||||||||||||||||
Displacement parameters | Biso mean: 35.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.8→19.92 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.8→2.97 Å / Rfactor Rfree error: 0.018 / Total num. of bins used: 6
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Xplor file |
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