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- PDB-2adr: ADR1 DNA-BINDING DOMAIN FROM SACCHAROMYCES CEREVISIAE, NMR, 25 ST... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2adr | ||||||
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Title | ADR1 DNA-BINDING DOMAIN FROM SACCHAROMYCES CEREVISIAE, NMR, 25 STRUCTURES | ||||||
![]() | ADR1 | ||||||
![]() | TRANSCRIPTION REGULATION / ADR1 / ZINC FINGER | ||||||
Function / homology | ![]() positive regulation of transcription from RNA polymerase II promoter by oleic acid / peroxisome organization / TFIID-class transcription factor complex binding / TFIIB-class transcription factor binding / chromatin organization / RNA polymerase II-specific DNA-binding transcription factor binding / sequence-specific DNA binding / nucleic acid binding / molecular adaptor activity / transcription coactivator activity ...positive regulation of transcription from RNA polymerase II promoter by oleic acid / peroxisome organization / TFIID-class transcription factor complex binding / TFIIB-class transcription factor binding / chromatin organization / RNA polymerase II-specific DNA-binding transcription factor binding / sequence-specific DNA binding / nucleic acid binding / molecular adaptor activity / transcription coactivator activity / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / chromatin / regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / nucleus / metal ion binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / DISTANCE GEOMETRY, SIMULATED ANNEALING | ||||||
![]() | Bowers, P.M. / Kleivt, R.E. | ||||||
![]() | ![]() Title: A folding transition and novel zinc finger accessory domain in the transcription factor ADR1. Authors: Bowers, P.M. / Schaufler, L.E. / Klevit, R.E. #1: ![]() Title: A Disorder-to-Order Transition Couled to DNA Binding in the Essential Zinc-Finger DNA-Binding of Yeast Adr1 Elucidated by Backbone Dynamics and Proton Exchange Authors: Hyre, D.E. / Klevit, R.E. #2: ![]() Title: NMR Chemical Shift Perturbation Mapping of DNA Binding by a Zinc-Finger Domain from the Yeast Transcription Factor Adr1 Authors: Schmiedeskamp, M. / Rajagopal, P. / Klevit, R.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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PDBx/mmCIF format | ![]() | 491.1 KB | Display | ![]() |
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PDB format | ![]() | 405.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 347.4 KB | Display | ![]() |
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Full document | ![]() | 495.7 KB | Display | |
Data in XML | ![]() | 25.9 KB | Display | |
Data in CIF | ![]() | 43.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 7126.188 Da / Num. of mol.: 1 / Fragment: DNA-BINDING DOMAIN / Mutation: C140A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Cell line: BL21 / Plasmid: PET11A / Species (production host): Escherichia coli / Production host: ![]() ![]() |
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#2: Chemical |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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Sample preparation
Sample conditions | pH: 5.5 / Temperature: 303 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DMX750 / Manufacturer: Bruker / Model: DMX750 / Field strength: 750 MHz |
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Processing
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NMR software |
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Refinement | Method: DISTANCE GEOMETRY, SIMULATED ANNEALING / Software ordinal: 1 Details: REFINEMENT DETAILS HAVE BEEN SUBMITTED FOR PUBLICATION. | ||||||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST ENERGY TERM / Conformers calculated total number: 50 / Conformers submitted total number: 25 |