+Open data
-Basic information
Entry | Database: PDB / ID: 2a9g | ||||||
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Title | Structure of C406A arginine deiminase in complex with L-arginine | ||||||
Components | Arginine deiminase | ||||||
Keywords | HYDROLASE / arginine degradation pathway / L-arginine deiminase / catalytic mechanism | ||||||
Function / homology | Function and homology information arginine deiminase / arginine deiminase activity / arginine deiminase pathway / arginine catabolic process to ornithine / cytoplasm Similarity search - Function | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.3 Å | ||||||
Authors | Galkin, A. / Lu, X. / Dunaway-Mariano, D. / Herzberg, O. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2005 Title: Crystal Structures Representing the Michaelis Complex and the Thiouronium Reaction Intermediate of Pseudomonas aeruginosa Arginine Deiminase. Authors: Galkin, A. / Lu, X. / Dunaway-Mariano, D. / Herzberg, O. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2a9g.cif.gz | 343.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2a9g.ent.gz | 279 KB | Display | PDB format |
PDBx/mmJSON format | 2a9g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a9/2a9g ftp://data.pdbj.org/pub/pdb/validation_reports/a9/2a9g | HTTPS FTP |
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-Related structure data
Related structure data | 2aafC 2abrC 2aciC 1rxxS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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Details | The biological assembly is a tetramer generated from the molecules A, B, C and D in the asymmetric unit |
-Components
#1: Protein | Mass: 46458.824 Da / Num. of mol.: 4 / Mutation: C406A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Gene: arcA / Plasmid: PET100-ADIN / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)Star / References: UniProt: P13981, arginine deiminase #2: Chemical | ChemComp-ARG / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 45 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.6 Details: 35% MPD (2-methyl-2,4-pentanediol), 6% PEG 3350, 0.1 M Tris-HCl, L_arginine 0.02 M, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU / Wavelength: 1.54178 Å |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Nov 21, 2003 |
Radiation | Monochromator: Osmic mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54178 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→20 Å / Num. all: 74422 / Num. obs: 73975 / % possible obs: 99.4 % / Rmerge(I) obs: 0.097 |
Reflection shell | Resolution: 2.3→2.38 Å / Rmerge(I) obs: 0.27 / % possible all: 96.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1RXX Resolution: 2.3→20 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 37 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.3→2.38 Å
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