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- PDB-27jp: Crystal structure of the mature form of human ASPRV1 -

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Basic information

Entry
Database: PDB / ID: 27jp
TitleCrystal structure of the mature form of human ASPRV1
ComponentsRetroviral-like aspartic protease 1
KeywordsHYDROLASE / Retroviral-like aspartic protease
Function / homology
Function and homology information


Hydrolases; Acting on peptide bonds (peptidases); Aspartic endopeptidases / skin development / protein processing / aspartic-type endopeptidase activity / membrane
Similarity search - Function
Retroviral-like aspartic protease 1 / gag-polyprotein putative aspartyl protease / Aspartyl protease, retroviral-type family profile. / Peptidase A2A, retrovirus, catalytic / Aspartic peptidase, active site / Eukaryotic and viral aspartyl proteases active site. / Aspartic peptidase domain superfamily
Similarity search - Domain/homology
Retroviral-like aspartic protease 1
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.35 Å
AuthorsChen, Z. / Feng, X. / Ding, J.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
Citation
Journal: Acta Biochim.Biophys.Sin. / Year: 2026
Title: Structure and enzymatic properties of human retroviral-like aspartic protease 1 and functional roles of disease-associated mutations.
Authors: Feng, X. / Chen, Z. / Lan, C. / Ding, J.
#1: Journal: Acta Crystallogr D Struct Biol / Year: 2019
Title: Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix.
Authors: Dorothee Liebschner / Pavel V Afonine / Matthew L Baker / Gábor Bunkóczi / Vincent B Chen / Tristan I Croll / Bradley Hintze / Li Wei Hung / Swati Jain / Airlie J McCoy / Nigel W Moriarty ...Authors: Dorothee Liebschner / Pavel V Afonine / Matthew L Baker / Gábor Bunkóczi / Vincent B Chen / Tristan I Croll / Bradley Hintze / Li Wei Hung / Swati Jain / Airlie J McCoy / Nigel W Moriarty / Robert D Oeffner / Billy K Poon / Michael G Prisant / Randy J Read / Jane S Richardson / David C Richardson / Massimo D Sammito / Oleg V Sobolev / Duncan H Stockwell / Thomas C Terwilliger / Alexandre G Urzhumtsev / Lizbeth L Videau / Christopher J Williams / Paul D Adams /
Abstract: Diffraction (X-ray, neutron and electron) and electron cryo-microscopy are powerful methods to determine three-dimensional macromolecular structures, which are required to understand biological ...Diffraction (X-ray, neutron and electron) and electron cryo-microscopy are powerful methods to determine three-dimensional macromolecular structures, which are required to understand biological processes and to develop new therapeutics against diseases. The overall structure-solution workflow is similar for these techniques, but nuances exist because the properties of the reduced experimental data are different. Software tools for structure determination should therefore be tailored for each method. Phenix is a comprehensive software package for macromolecular structure determination that handles data from any of these techniques. Tasks performed with Phenix include data-quality assessment, map improvement, model building, the validation/rebuilding/refinement cycle and deposition. Each tool caters to the type of experimental data. The design of Phenix emphasizes the automation of procedures, where possible, to minimize repetitive and time-consuming manual tasks, while default parameters are chosen to encourage best practice. A graphical user interface provides access to many command-line features of Phenix and streamlines the transition between programs, project tracking and re-running of previous tasks.
History
DepositionJun 3, 2026Deposition site: PDBJ / Processing site: PDBC
Revision 1.0Sep 2, 2026Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Retroviral-like aspartic protease 1
B: Retroviral-like aspartic protease 1
C: Retroviral-like aspartic protease 1
D: Retroviral-like aspartic protease 1
E: Retroviral-like aspartic protease 1
F: Retroviral-like aspartic protease 1
G: Retroviral-like aspartic protease 1
H: Retroviral-like aspartic protease 1


Theoretical massNumber of molelcules
Total (without water)119,6808
Polymers119,6808
Non-polymers00
Water7,296405
1
A: Retroviral-like aspartic protease 1
G: Retroviral-like aspartic protease 1


Theoretical massNumber of molelcules
Total (without water)29,9202
Polymers29,9202
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Retroviral-like aspartic protease 1
E: Retroviral-like aspartic protease 1


Theoretical massNumber of molelcules
Total (without water)29,9202
Polymers29,9202
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: Retroviral-like aspartic protease 1
D: Retroviral-like aspartic protease 1


Theoretical massNumber of molelcules
Total (without water)29,9202
Polymers29,9202
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
F: Retroviral-like aspartic protease 1
H: Retroviral-like aspartic protease 1


Theoretical massNumber of molelcules
Total (without water)29,9202
Polymers29,9202
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)260.139, 34.975, 185.550
Angle α, β, γ (deg.)90.000, 134.345, 90.000
Int Tables number5
Space group name H-MC121
Space group name HallC2y
Symmetry operation#1: x,y,z
#2: -x,y,-z
#3: x+1/2,y+1/2,z
#4: -x+1/2,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails (eV)
d_1ens_1(chain "A" and (resid 193 through 196 or resid 198...
d_2ens_1(chain "B" and (resid 193 through 196 or resid 198...
d_3ens_1(chain "C" and (resid 193 through 196 or resid 198...
d_4ens_1(chain "D" and (resid 193 through 196 or resid 198...
d_5ens_1(chain "E" and (resid 193 through 196 or resid 198...
d_6ens_1(chain "F" and (resid 193 through 196 or resid 198...
d_7ens_1(chain "G" and (resid 193 through 196 or resid 198...
d_8ens_1(chain "H" and (resid 193 through 196 or resid 198...

NCS domain segments:

Ens-ID: ens_1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
d_11GLYGLYTYRTYRAA193 - 1964 - 7
d_12LEULEUVALVALAA198 - 2079 - 18
d_13PHEPHELEULEUAA209 - 23620 - 47
d_14PROPROPHEPHEAA238 - 23949 - 50
d_15ILEILEGLYGLYAA253 - 26464 - 75
d_16ALAALAALAALAAA270 - 27581 - 86
d_17ALAALAALAALAAA27788
d_18ALAALALEULEUAA279 - 28990 - 100
d_19ASPASPHISHISAA291 - 300102 - 111
d_110THRTHRPHEPHEAA302 - 310113 - 121
d_111LEULEUVALVALAA312 - 315123 - 126
d_21GLYGLYTYRTYRBB193 - 1964 - 7
d_22LEULEUVALVALBB198 - 2079 - 18
d_23PHEPHELEULEUBB209 - 23620 - 47
d_24PROPROPHEPHEBB238 - 23949 - 50
d_25ILEILEGLYGLYBB253 - 26464 - 75
d_26ALAALAALAALABB270 - 27581 - 86
d_27ALAALAALAALABB27788
d_28ALAALALEULEUBB279 - 28990 - 100
d_29ASPASPHISHISBB291 - 300102 - 111
d_210THRTHRPHEPHEBB302 - 310113 - 121
d_211LEULEUVALVALBB312 - 315123 - 126
d_31GLYGLYTYRTYRCC193 - 1964 - 7
d_32LEULEUVALVALCC198 - 2079 - 18
d_33PHEPHELEULEUCC209 - 23620 - 47
d_34PROPROPHEPHECC238 - 23949 - 50
d_35ILEILEGLYGLYCC253 - 26464 - 75
d_36ALAALAALAALACC270 - 27581 - 86
d_37ALAALAALAALACC27788
d_38ALAALALEULEUCC279 - 28990 - 100
d_39ASPASPHISHISCC291 - 300102 - 111
d_310THRTHRPHEPHECC302 - 310113 - 121
d_311LEULEUVALVALCC312 - 315123 - 126
d_41GLYGLYTYRTYRDD193 - 1964 - 7
d_42LEULEUVALVALDD198 - 2079 - 18
d_43PHEPHELEULEUDD209 - 23620 - 47
d_44PROPROGLYGLYDD238 - 26449 - 75
d_45ALAALAALAALADD270 - 27581 - 86
d_46ALAALAALAALADD27788
d_47ALAALALEULEUDD279 - 28990 - 100
d_48ASPASPHISHISDD291 - 300102 - 111
d_49THRTHRPHEPHEDD302 - 310113 - 121
d_410LEULEUVALVALDD312 - 315123 - 126
d_51GLYGLYTYRTYREE193 - 1964 - 7
d_52LEULEUVALVALEE198 - 2079 - 18
d_53PHEPHELEULEUEE209 - 23620 - 47
d_54PROPROGLYGLYEE238 - 26449 - 75
d_55ALAALAALAALAEE270 - 27581 - 86
d_56ALAALAALAALAEE27788
d_57ALAALALEULEUEE279 - 28990 - 100
d_58ASPASPHISHISEE291 - 300102 - 111
d_59THRTHRPHEPHEEE302 - 310113 - 121
d_510LEULEUVALVALEE312 - 315123 - 126
d_61GLYGLYTYRTYRFF193 - 1964 - 7
d_62LEULEUVALVALFF198 - 2079 - 18
d_63PHEPHELEULEUFF209 - 23620 - 47
d_64PROPROGLYGLYFF238 - 26449 - 75
d_65ALAALAALAALAFF270 - 27581 - 86
d_66ALAALAALAALAFF27788
d_67ALAALALEULEUFF279 - 28990 - 100
d_68ASPASPHISHISFF291 - 300102 - 111
d_69THRTHRPHEPHEFF302 - 310113 - 121
d_610LEULEUVALVALFF312 - 315123 - 126
d_71GLYGLYTYRTYRGG193 - 1964 - 7
d_72LEULEUVALVALGG198 - 2079 - 18
d_73PHEPHELEULEUGG209 - 23620 - 47
d_74PROPROGLYGLYGG238 - 26449 - 75
d_75ALAALAALAALAGG270 - 27581 - 86
d_76ALAALAALAALAGG27788
d_77ALAALALEULEUGG279 - 28990 - 100
d_78ASPASPHISHISGG291 - 300102 - 111
d_79THRTHRPHEPHEGG302 - 310113 - 121
d_710LEULEUVALVALGG312 - 315123 - 126
d_81GLYGLYTYRTYRHH193 - 1964 - 7
d_82LEULEUVALVALHH198 - 2079 - 18
d_83PHEPHELEULEUHH209 - 23620 - 47
d_84PROPROGLYGLYHH238 - 26449 - 75
d_85ALAALAALAALAHH270 - 27581 - 86
d_86ALAALAALAALAHH27788
d_87ALAALALEULEUHH279 - 28990 - 100
d_88ASPASPHISHISHH291 - 300102 - 111
d_89THRTHRPHEPHEHH302 - 310113 - 121
d_810LEULEUVALVALHH312 - 315123 - 126

NCS oper:
IDCodeMatrixVector
1given(0.129051458066, -0.0234523978507, -0.991360532907), (-0.0346832461754, 0.999001880397, -0.0281480976051), (0.991031176909, 0.0380161544491, 0.128109243987)62.274055254, -11.6231124052, -9.06116797128
2given(0.013564196217, 0.0172066169943, 0.999759943643), (-0.027026414581, 0.999492943532, -0.0168353421083), (-0.999542688181, -0.0267915688346, 0.014022351568)2.84526268297, -19.7633654762, 67.1620166286
3given(0.392483178087, 0.0937518572039, -0.914968602843), (0.584265338056, -0.793707792815, 0.1692984181), (-0.71034566914, -0.601031221232, -0.366292917539)113.111365264, -61.1688309142, 78.7807271932
4given(-0.297127171776, -0.0377103482079, 0.954092958485), (0.579965960144, -0.800906084478, 0.148959487514), (0.758521541465, 0.597601349969, 0.259841678045)-47.9053480214, -52.6676134162, -6.88594769541
5given(-0.757771731538, -0.600291583292, -0.25579682936), (0.584441707328, -0.798724948158, 0.143060644225), (-0.290189409898, -0.0410910235915, 0.956086624822)69.8959393358, -30.1486334879, -53.6535975581
6given(0.697085927939, 0.586091023221, 0.412999420786), (0.587387664689, -0.797139889462, 0.139798884113), (0.411153183668, 0.145138930437, -0.899937636968)-13.3607012877, -37.538388781, 108.422552399
7given(-0.978881026604, 0.00292050621724, -0.204409897993), (0.00179902893949, 0.999982295625, 0.00567203069764), (0.204422844245, 0.00518450391023, -0.978868950202)77.0912013486, 6.99152752659, 53.6903166527

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Components

#1: Protein
Retroviral-like aspartic protease 1 / Skin-specific retroviral-like aspartic protease / SASPase / Skin aspartic protease / TPA-inducible ...Skin-specific retroviral-like aspartic protease / SASPase / Skin aspartic protease / TPA-inducible aspartic proteinase-like protein / TAPS


Mass: 14960.032 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ASPRV1, SASP / Production host: Escherichia coli BL21(DE3) (bacteria)
References: UniProt: Q53RT3, Hydrolases; Acting on peptide bonds (peptidases); Aspartic endopeptidases
#2: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 405 / Source method: isolated from a natural source / Formula: H2O
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.53 Å3/Da / Density % sol: 51.37 %
Crystal growTemperature: 289 K / Method: vapor diffusion, hanging drop
Details: 0.2 M magnesium formate dihydrate, pH 7.0, and 20% (v/v) PEG 3,350

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Data collection

DiffractionMean temperature: 193 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: NFPSS / Beamline: BL18U / Wavelength: 0.97853 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Dec 1, 2025
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97853 Å / Relative weight: 1
ReflectionResolution: 2.35→132.69 Å / Num. obs: 50963 / % possible obs: 99.7 % / Redundancy: 5.9 % / Biso Wilson estimate: 32.87 Å2 / CC1/2: 0.998 / Net I/σ(I): 12.4
Reflection shellResolution: 2.35→2.42 Å / Num. unique obs: 4328 / CC1/2: 0.856

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Processing

SoftwareName: PHENIX / Version: 1.21.2_5419 / Classification: refinement
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.35→132.69 Å / SU ML: 0.3015 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 25.5858
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2612 2550 5.02 %
Rwork0.2044 48223 -
obs0.2072 50773 99.29 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 40.15 Å2
Refinement stepCycle: LAST / Resolution: 2.35→132.69 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms7152 0 0 405 7557
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00817280
X-RAY DIFFRACTIONf_angle_d1.06589848
X-RAY DIFFRACTIONf_chiral_restr0.06581152
X-RAY DIFFRACTIONf_plane_restr0.00851240
X-RAY DIFFRACTIONf_dihedral_angle_d17.47072620
Refine LS restraints NCS
Ens-IDDom-IDAsym-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AAX-RAY DIFFRACTIONTorsion NCS0.593848837321
ens_1d_3AAX-RAY DIFFRACTIONTorsion NCS0.596807445917
ens_1d_4AAX-RAY DIFFRACTIONTorsion NCS0.958496416159
ens_1d_5AAX-RAY DIFFRACTIONTorsion NCS0.838779395576
ens_1d_6AAX-RAY DIFFRACTIONTorsion NCS0.991254351914
ens_1d_7AAX-RAY DIFFRACTIONTorsion NCS0.94264776878
ens_1d_8AAX-RAY DIFFRACTIONTorsion NCS0.630737248876
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.35-2.40.35221530.28352567X-RAY DIFFRACTION99.34
2.4-2.440.33591470.25832659X-RAY DIFFRACTION99.68
2.44-2.50.32771390.25522709X-RAY DIFFRACTION99.79
2.5-2.560.28681510.24342609X-RAY DIFFRACTION99.78
2.56-2.620.31411630.23592630X-RAY DIFFRACTION99.89
2.62-2.690.27741720.22672699X-RAY DIFFRACTION99.65
2.69-2.770.29251410.22962581X-RAY DIFFRACTION99.67
2.77-2.860.3291990.21622735X-RAY DIFFRACTION99.82
2.86-2.960.28831190.22242712X-RAY DIFFRACTION99.61
2.96-3.080.26421680.22422591X-RAY DIFFRACTION99.6
3.08-3.220.30291450.22992708X-RAY DIFFRACTION99.72
3.22-3.390.27641240.20992670X-RAY DIFFRACTION99.54
3.39-3.60.26311180.19882678X-RAY DIFFRACTION97.73
3.6-3.880.27771500.2192632X-RAY DIFFRACTION96.6
3.88-4.270.21861260.16352665X-RAY DIFFRACTION99.01
4.27-4.890.18381310.1422760X-RAY DIFFRACTION99.55
4.89-6.160.22471180.17732780X-RAY DIFFRACTION99.83
6.16-132.690.2321860.20962838X-RAY DIFFRACTION98.5
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.9341272443190.6794564799960.4451315907641.04521168607-0.3259609872253.40236980221-0.061310700639-0.1727999804340.254402473470.264006077725-0.02521466960920.333436432344-0.575235233374-0.263702192955-0.07799343907080.2383154658070.02257473636240.07208572835810.244448945917-0.1135238485480.35788064917829.71205838276.1011879984560.5378782153
22.70102828826-0.1139728041030.8648169879213.636423601610.1860212239440.3104484490610.029082542646-0.1441112500120.2990744530630.2426287143650.399837249480.425920519271-0.691947130973-0.1845210245240.007086855781450.254054194240.07266041727190.04144245661510.179117203464-0.08011769545180.35567462302627.9225436758.6255787029752.7696417737
32.58559119348-0.3423880778211.412856581472.71886883302-0.1403453601272.997287601620.157994961080.0849286424078-0.3959416585090.2251166488590.1820553239540.765548958160.33163369859-0.2974832035690.01586045888460.23253639178-0.05048499433660.04517151256160.280013507573-0.03180138723770.29817059806827.7774109542-4.8019629127955.1561419124
41.700131700140.5923562947370.2812889684762.72334189528-0.7818474480892.15361598203-0.2720095025380.5211851882091.03922328695-0.1617198001010.5500778756611.25724651797-0.418235225427-0.3840546173690.1365340963410.224558706338-0.0760115685613-0.1195236915120.2391699045490.04626855731650.57359306918622.43311717574.664059320740.6323748795
54.991953427520.0837951939793-1.736783669081.57022495466-2.286812748423.907494512090.1303466241320.497466194154-1.200654756750.200377846866-0.6451272389420.2612000210651.38969951650.104493300251-0.1719019290350.613848782086-0.130843104814-0.00497438306960.249096119152-0.003579647293770.40429891457823.4144521013-10.811980258643.756600879
61.703809172540.5550931735391.540856985151.84926366376-0.650444752053.610121196610.2501557336890.05230354693150.0603314488522-0.0480024881012-0.07668862524620.2292908228820.0142101898739-0.03018979630.02425528673360.20551891776-0.0136048856417-0.03835817847040.222260139058-0.05024392127240.22313913829529.2997469260.63399746609449.080453409
71.390747505-0.1902628520291.193725786822.32826226056-0.5435201033941.779806335680.04217557994620.03429287785210.255538384240.418452001699-0.0981504968503-0.0481895341431-0.0440792887050.516828004545-0.05003671270190.266574954523-0.04743858941240.01060085614170.29957054694-0.07772070977080.18871224598838.67257218812.0715892647661.5669167403
83.07452269668-0.397488685545-1.117531061031.561688776730.511925058372.635090086790.07531583665420.3622745298450.280280788563-0.255611975422-0.09413296084940.185345210126-0.428022740125-0.096460626119-0.03085336052590.307406275637-0.0284250515511-0.04176171575250.216011487740.007326406883260.19884099590415.0483474932-10.384065183223.874730673
94.291593350312.013554568960.3210458225182.54359321409-0.3218485046960.5643532888820.5161784937920.541552578979-2.061544498230.0271329544114-0.5372004991430.07956144259880.4980948818320.254561253443-0.0343489980760.457873669379-0.0644510339718-0.006970412898390.355667677633-0.1525674665620.58081557661421.948497482-24.458258143819.5589439811
101.76315310084-0.144369267136-0.8616323913631.902547332591.025378390542.405233238150.00794324554796-0.170672066436-0.2599743110820.260857757372-0.0887660584243-0.1180860047030.3922334421890.323922835682-0.04918781389660.216168484389-0.026597736617-0.03332074911120.2297525408050.0001279692573480.20968586588120.0914762115-12.788993648328.5199702724
114.445311199620.672365927142-0.7669398272822.986402613630.948816483681.957844374730.1689323591060.1446196927680.077588760575-0.259235305996-0.2785501563940.284504675897-0.06792545137-0.122789129165-0.002252596809990.23848855044-0.0227191637954-0.06269346168310.189955704698-0.02138071104030.2111247716837.2499021906-13.065405846233.5950584314
122.08622678277-1.300205384380.0150152581751.147226842870.5823727865172.093153370970.0712131349704-0.297235385690.801381476030.2294704874730.130674862432-0.208610217319-0.2398737734280.3388709798680.06123810394930.28374124928-0.04849541883530.01491356493360.2542567149240.01981743351690.44567058628259.6956703047-18.587770618139.3299274511
134.389557141650.677643910462-1.440819807860.484559005017-1.482101504074.330878237060.183302429422-0.0847514147030.2029050872780.0362597423198-0.1952247093170.196631994744-0.1774094140220.153566366748-0.002728414753590.2696361726930.005536614636470.07652608878360.2312771163340.002090556814860.39556653353647.3939168727-21.728585807140.7867223402
142.75847890233-0.1857217976550.6593279877224.104029872510.3695637155221.142861365350.291734559340.01080919015970.6017873973140.0754511812121-0.272005096493-0.416449166232-0.05451247155650.0306537740816-0.0291859840390.253979109585-0.05428218622480.0367268995720.2447398546160.09708243053990.38162750565463.3572349603-20.173956475332.7233847009
151.56662852127-1.96092465488-0.3726341961842.486193622890.4356940009511.127208303390.43824237129-0.666792524421-0.4650211701710.2096069929520.339626669431.16608818475-0.734635453754-1.15154261275-0.0003329743551910.313561177530.1006907375180.07736578677940.5934855871880.1325965736930.63601517993557.3123957584-33.805014920631.7024533675
164.94481445975-0.09141421115051.24520408533.771035839320.7125532080717.10364779587-0.289500589887-0.217950183598-1.209812255970.1908104282190.5965325879860.8197376576660.0808177532050.4446933641310.6378388550140.231384208799-0.0415432084240.05057414387890.3118148420720.2084659511720.44327505968377.2629537988-42.039835243432.1057237229
171.80378099653-1.546671562040.6196331068341.241497743420.03262073098180.525416131435-0.0988744761606-0.505316785932-0.2594050408950.1931815724120.1206980083770.1177876805930.21072741196-0.17135773142-0.01866330018250.230432832997-0.032051224522-0.01036673006510.3767077378240.1203156181670.43503776244576.3695025331-39.124713308240.9727459836
183.16415748513-0.018021908641-0.5982686793273.886307564071.093346179052.11802882989-0.197845421957-0.223677793761-0.1392138016280.4944002193220.35204949578-0.25919081173-0.333091560734-0.1720615185250.1383414709720.4261959625580.125889133754-0.07454615166570.4276519503280.1813537960210.33484775965487.1825372621-38.978856951549.2461942686
191.350647172910.03271981942811.118928039450.8836315276780.5349437310695.89674658899-0.1252440413730.1032424336060.03114821625620.275806542791-0.0211356946693-0.3231146398640.523022355010.8521531607970.01546163114470.3434259413530.018536744728-0.02670557948470.3112454577760.07840958659290.32421839992884.2958512929-36.800910564138.2038403026
202.243235317190.738054271312-0.8760447952332.41047291152-0.6592974321423.49009033363-0.131673867426-0.132422840051-0.05115251663790.1477036838870.09359093343880.07515996026660.1310189300551.03089778663-0.02609869566960.22306655930.0205417090542-0.05459860860240.2966420712020.06368271648830.31787909876783.2482772045-34.890204769134.7036834633
211.100848431021.299588991791.364037281221.710842715071.502607483761.93708980967-1.07600017791-0.976583347333-1.45120584863-0.505720045423-0.1090777183350.768270710323-0.0256413558248-0.304982001078-0.1412641778180.9135588346420.1263437584220.1989041303760.9907557341060.2553023527940.89430300206871.7460520715-40.148009987845.6186685762
222.02265884482-0.512369855817-1.189652645182.32634013993-0.2441266302991.86740515423-0.04567117815050.3239187377380.1983089266180.03977819405980.01713300937390.0623435658748-0.206547362265-0.04315438571620.005261459297670.222311095411-0.009006990630510.03699062336730.3271660418450.01809702845240.29013350861572.5387999371-28.204621189129.9530095116
230.383772514811-0.773481728958-1.021092734483.99682789528-0.3825929876034.764799773410.7470969459131.066270362351.065484652290.354693962452-0.1529611158310.7136216150990.160292437649-0.2242639818240.09359236390690.2827663422090.03469500759990.1290625674050.526903270460.09909876929530.46373845140176.415030105-31.615420833622.7113467572
242.580644589322.354379485630.8530067818443.92081133392-0.112014214891.090456097870.2668979871280.340939519178-0.1238536483910.402678773619-0.05087166869040.4002613759120.9882661279510.857216989248-0.04591482558680.441393742191-0.0317683233938-0.03474226734170.5654911610.05093299904620.41848742388464.5211495631-40.561440443527.8107190149
255.515811122782.583753899460.004925023562961.46815754602-0.4059023867741.257463419030.4066282418730.202483903362-1.942312595780.00542140773344-0.578317107581-0.7892297363810.2501855810450.311099066306-0.01119377329430.267933754526-0.00686219427664-0.02541710102220.223580177091-0.09860401459470.5596449644594.53108139536-30.077462040435.069142219
261.66094074601-0.4730956643861.571691674954.122691882410.7945989572183.29425421435-0.0736091476258-0.24186817679-0.343956899541-0.1998009499710.104656589126-0.2672246746810.118680238915-0.07467009311060.02455847503410.288866373345-0.0394869613253-0.0101137423280.311812900565-0.02224322873570.367377813846-6.18751071277-35.466875413132.907933501
272.194243411151.201268280950.3137460488071.267382710920.08805687146670.97835663914-0.2701183939930.570196277951-0.645427936086-0.4504211392230.261983083364-0.259173902463-0.05092507890260.154184134759-0.0194376219110.258724510756-0.0411878414347-0.01552752479470.307666028011-0.06238771148510.375915776368-7.62448431309-30.937534518924.9263986358
282.433697870740.232391454661-1.243142776593.68359599332-0.3257222097961.55891547537-1.214882491660.117741607603-0.2439693848740.3437198616230.8424183807180.0634592748174-0.3806983671210.6601835970730.03339045367830.320369256286-0.208754390365-0.141103187520.40710090821-0.0926237660270.396986596663-18.2396191271-31.382588783719.7022613881
292.832850953321.075927109981.444969469862.23144602058-0.4637986594143.16617558799-0.4480087371440.399722610996-0.513400969438-0.2677313721010.0535701046834-0.008646254320870.2275112314240.3554594012720.002143881964480.244447711002-0.0211087261310.0185710209390.341166012031-0.07506208586060.354264353321-9.92935999144-28.867124608129.2447723204
302.399461717691.01273281169-0.7191860907262.428214187770.4952807962852.319303645960.0920824003202-0.345527784451-0.01947179659150.429187766097-0.097241169312-0.0489349743612-0.2724091615240.02119785033630.005881395189360.254609793749-0.05016939773160.006093475158840.2714647307830.01394026445490.318804901745-1.13089269669-20.415291952338.7752825828
314.53729266150.409657302361-1.056977532621.170551026961.967621074523.999810943920.0902658333697-0.8163081165280.330092219312-0.0836127060425-0.450512195789-0.114507834318-0.8429258552280.3323051116260.02013792236070.468440322326-0.04106478778350.1624485228340.486997161372-0.04679917025310.493891285624-4.91383624308-25.088460152844.9117102865
321.18613752-1.268931097490.1996672338161.60128242804-0.2311388980820.09868223352790.3688051310210.18359543796-0.650933749-0.0255696187546-0.4452478650710.8927498627420.0195288355172-0.266825139081-0.006557543469350.290876860441-0.0437477697562-0.09778615836820.2633801775910.01962345568290.3300751753556.64358612255-33.400786808138.4553465508
330.885037353074-0.5286638638190.4533863512462.174535949710.1348391343022.86017305825-0.598765002784-1.369947158730.04077396379330.8173934830460.5416838557390.06819075033440.2324225634730.0621379040210.1072288785931.28233897769-0.234406025830.320808925184-0.908580537821.40579327976-0.43442582118830.0641573493-4.5515781218.02148243163
342.3794474579-0.538943639305-1.509179822810.522391372091.88101364377.483243426260.05117432483931.021589761490.4131908096950.4745164427170.9302430324430.3119502973851.98570017578-0.6700091244440.3049720989250.3648725625570.0996561456682-0.02887374108230.2886084658470.04242018604320.32883705249327.1640630078-12.666558309-12.1530884747
351.079966745390.08349244195260.4531796574691.91597529131.210293931881.674453732480.1196160606810.04591139246140.2962155964350.6321781557820.0649419371599-0.02474199421240.3324405713780.176541694339-0.002503780574110.3801051754560.06050030785230.04691512208030.2204672754830.005020921462340.16248729067134.7213681761-6.18323852214-9.21851293551
365.765731864712.31355288184-1.23646909011.17942077338-1.566390279435.10365147523-0.2882238820370.183717960252-0.6290377744640.8533722056490.529418879038-0.7128353840261.544852218780.463851683237-0.03413695288220.3965799377680.142659401357-0.041546409210.274774407957-0.006949592037770.2510424611939.2654996836-15.9375629945-17.959651159
372.608011498170.0901723230655-1.164654145260.6543693560430.1346322073062.097470933870.2215059165980.2080027125170.262329716407-0.08965888144950.1894797444220.15607023077-0.2201141664870.0498179164221-0.01386187696550.2160838541940.0474387118307-0.005645863103460.2619569907440.02069037832660.22609476483739.3110024295-8.15585511024-22.0120411081
380.912731232546-1.178415334160.2554935456151.40955556779-0.5120863997521.19137799051-0.2825080729920.1083866228321.071744781230.09878690102470.1947935019160.2846988336440.0525325195982-0.1581730730080.01024828541720.4506698440140.04541524038920.04468208342220.2920684486980.0148352066760.38210484076929.778896548-5.09764635462-18.24899601
391.924448090670.8221294141790.3164045402052.356319314471.118305551233.590764404470.21063243821-0.300501959646-0.435426940017-0.04545291465790.0673463836027-0.04017005071760.1301800052090.189744697065-0.006353200855920.4398860038880.07754319747640.03166308630870.2852928331540.02953873675380.22104924863129.0019874867-1.04210571272-6.94361873948
403.0918737025-1.591042705971.267315359692.78760627912-0.7058683464485.04237438588-0.003434929203240.311283852128-0.3655995068830.3508811884790.3984510892790.246134416756-1.61071359133-1.57043124163-0.1732290190340.4129716478080.120521138104-0.02624882869720.4901218826230.0003182747408720.43933800263618.2519049394-2.13677045537-9.78199109897
412.687908840411.461048264341.850981231572.96853670608-0.4512045066782.322072504540.935649348088-0.42775330601-0.2825992823670.0863583160081-0.577210233645-0.6562568380350.414267987165-0.4750396482150.08454093281810.539345821760.1224397518850.02800184329370.4984415684360.005356306427650.31259606000624.9979230697-11.29715700241.5730998974
422.90911690965-0.594933895286-0.6460817052762.561792653050.1053828040192.038904982120.1779377761830.2393984797850.0258704145804-0.142957972751-0.03539142316520.06016956287720.199549159277-0.108416881130.04188135845520.377031110403-0.0533128197366-0.02603838353840.232559021306-0.09070316666380.19854947403232.0995516234-14.961434419867.9966203303
432.31255244994-0.358061375587-1.019807970280.7412013727780.3629295568513.14574956259-0.4118269088730.0353640481348-0.215446263546-0.08756929730490.398805720269-0.02965661628360.244670329249-0.4925473793910.02362373761480.4736857764-0.08174343620180.1054824477850.3411944521880.001769024388780.32555406167219.7824266487-19.332506953881.2322344286
441.542429587920.143884863845-1.10676783551.66555670674-1.500737235543.489767816810.1161695151590.205838989505-0.07402301531080.285038840202-0.06685937754840.1349009153610.0554213936435-0.1570182410040.004002751643150.377704211934-0.03686439953460.002154459138730.274050602214-0.115270686160.22027397076233.2133495461-10.461154435473.5856205816
454.961474107033.22858678725-0.1222766231835.50868361793.094967219856.28300962912-0.0328683494764-0.369258024496-1.18277604362-0.5699443268290.125283109004-0.986798373925-0.8366873576411.29547829548-0.3053673470130.357988681555-0.0420230747686-0.0820262269590.429579590139-0.0458791061890.39670755419844.891510226-9.8003066697974.000557239
462.90829780089-0.0265974534013-0.9517949899512.6434419636-0.6275991792770.4688043458460.3195448525150.2648067640910.189730170280.109229619753-7.5544156335E-5-0.4007834648030.944717636179-0.009155536775280.4063317113840.448436154759-0.0152230110251-0.1233843774910.525292632356-0.1031648370690.22208332034940.1974723989-18.661822533561.5504440858
471.946639784360.2881425770310.04718359574321.080378530211.05628763433.085694006240.0347706337548-0.03087241642790.221449056303-0.5823735159670.1360492159450.145095179598-1.418561128880.284474987330.02001787296670.4191562403270.003941184311930.07682985532760.2161186975690.01370804220560.31944008647735.593795015913.56137058960.700803373772
482.2000672210.4730938356780.865415275922.453539682140.4642808455512.659649175920.104544789790.20219124541-0.03380103458580.01674841197610.215430466878-0.609865869696-0.09024212722850.767607904044-0.0218127580560.336781613484-0.0098731006651-0.02355344869390.3518975764350.01049171298320.29837979924338.72190278048.611925172756.50177587828
490.7679836094270.510083987405-1.401747931513.53477147513-3.989369900555.653944214750.423186212912-0.1541556290590.4087921823860.8590215516610.7021864392560.22318124287-0.6958362628510.8653123616670.3701786829230.443880939332-0.104685402873-0.2399225679040.7412721266280.1079253434670.69139760265946.992543054611.91795200618.3157520293
502.694713453570.171314264235-0.1720014891722.054216746150.6565948239052.897720350490.357278246987-0.5378156594530.06632107681370.631443432022-0.268827860616-0.6398455499120.4953168131310.158640640885-0.009416756020210.475139903025-0.0415374106112-0.1036925244960.4462396806920.05988733916450.40492118269437.38079956367.1691503638115.6088815654
516.07679021436-0.4013524011850.9015531689028.36279418026-0.8478373717512.855757556620.451269028453-0.181193240857-0.1182716631940.401019937633-0.77013078101-2.591327999230.1968868037350.999059414043-0.02314842508480.4369626412730.0211855917590.03277094086410.4794696071770.09876595436330.6372455537443.65311488136.388004546415.40715171715
522.88457499364-0.0667800209208-0.004757642693252.100883011691.821102205012.012494486060.150819825462-0.1114877359250.0839236193432-0.175583729945-0.182694654566-0.110121535071-0.0774973955115-0.41221350707-0.02101920663090.348663026770.08246093850450.05601197294040.3042652031080.073514808310.25399800843426.85985020889.550295779961.40901543062
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 193 through 202 )AA193 - 2021 - 10
22chain 'A' and (resid 203 through 210 )AA203 - 21011 - 18
33chain 'A' and (resid 211 through 221 )AA211 - 22119 - 29
44chain 'A' and (resid 222 through 235 )AA222 - 23530 - 43
55chain 'A' and (resid 236 through 252 )AA236 - 25244 - 53
66chain 'A' and (resid 253 through 285 )AA253 - 28554 - 86
77chain 'A' and (resid 286 through 316 )AA286 - 31687 - 117
88chain 'B' and (resid 193 through 235 )BB193 - 2351 - 43
99chain 'B' and (resid 236 through 252 )BB236 - 25244 - 53
1010chain 'B' and (resid 253 through 275 )BB253 - 27554 - 76
1111chain 'B' and (resid 276 through 316 )BB276 - 31677 - 117
1212chain 'C' and (resid 193 through 221 )CC193 - 2211 - 29
1313chain 'C' and (resid 222 through 275 )CC222 - 27530 - 76
1414chain 'C' and (resid 276 through 316 )CC276 - 31677 - 117
1515chain 'D' and (resid 190 through 196 )DD190 - 1961 - 7
1616chain 'D' and (resid 197 through 204 )DD197 - 2048 - 15
1717chain 'D' and (resid 205 through 229 )DD205 - 22916 - 40
1818chain 'D' and (resid 230 through 254 )DD230 - 25441 - 52
1919chain 'D' and (resid 255 through 263 )DD255 - 26353 - 61
2020chain 'D' and (resid 264 through 274 )DD264 - 27462 - 72
2121chain 'D' and (resid 275 through 281 )DD275 - 28173 - 79
2222chain 'D' and (resid 282 through 305 )DD282 - 30580 - 103
2323chain 'D' and (resid 306 through 311 )DD306 - 311104 - 109
2424chain 'D' and (resid 312 through 319 )DD312 - 319110 - 117
2525chain 'E' and (resid 190 through 202 )EE190 - 2021 - 13
2626chain 'E' and (resid 203 through 210 )EE203 - 21014 - 21
2727chain 'E' and (resid 211 through 229 )EE211 - 22922 - 40
2828chain 'E' and (resid 230 through 254 )EE230 - 25441 - 52
2929chain 'E' and (resid 255 through 285 )EE255 - 28553 - 83
3030chain 'E' and (resid 286 through 305 )EE286 - 30584 - 103
3131chain 'E' and (resid 306 through 311 )EE306 - 311104 - 109
3232chain 'E' and (resid 312 through 319 )EE312 - 319110 - 117
3333chain 'F' and (resid 190 through 196 )FF190 - 1961 - 7
3434chain 'F' and (resid 197 through 204 )FF197 - 2048 - 15
3535chain 'F' and (resid 205 through 221 )FF205 - 22116 - 32
3636chain 'F' and (resid 222 through 229 )FF222 - 22933 - 40
3737chain 'F' and (resid 230 through 263 )FF230 - 26341 - 61
3838chain 'F' and (resid 264 through 274 )FF264 - 27462 - 72
3939chain 'F' and (resid 275 through 305 )FF275 - 30573 - 103
4040chain 'F' and (resid 306 through 311 )FF306 - 311104 - 109
4141chain 'F' and (resid 312 through 319 )FF312 - 319110 - 117
4242chain 'G' and (resid 190 through 221 )GG190 - 2211 - 32
4343chain 'G' and (resid 222 through 254 )GG222 - 25433 - 52
4444chain 'G' and (resid 255 through 305 )GG255 - 30553 - 103
4545chain 'G' and (resid 306 through 311 )GG306 - 311104 - 109
4646chain 'G' and (resid 312 through 319 )GG312 - 319110 - 117
4747chain 'H' and (resid 193 through 202 )HH193 - 2021 - 10
4848chain 'H' and (resid 203 through 221 )HH203 - 22111 - 29
4949chain 'H' and (resid 222 through 235 )HH222 - 23530 - 43
5050chain 'H' and (resid 236 through 274 )HH236 - 27444 - 69
5151chain 'H' and (resid 275 through 285 )HH275 - 28570 - 80
5252chain 'H' and (resid 286 through 316 )HH286 - 31681 - 111

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