PDB-24pi: Ancestrally reconstructed acetolactate synthase - Ancestor N1774 ...

ID or keywords:

Entry

Database: PDB / ID: 24pi

Title

Ancestrally reconstructed acetolactate synthase - Ancestor N1774 with bound ThDP and magnesium.

Components

Ancestral acetolactate synthase, catabolic

Keywords

TRANSFERASE / ThDP / Ancestor

Function / homology

ACETATE ION / THIAMINE DIPHOSPHATE

Function and homology information

Biological species

synthetic construct (others)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.75 Å

Authors

Douw, A. / Guddat, L.W.

Funding support

Australia, 1items

Citation

Journal: To Be Published
Title: Engineering of Acetolactate Synthase for Isobutanol Production Using Ancestral Sequence Reconstruction
Authors: Douw, A. / Krco, S. / Bayaraa, T. / Foley, G. / Davis, S.J. / Paredes Trujillo, O. / Asser, L. / Sieber, V. / Boden, M. / Hine, D. / Guddat, L.W. / Schenk, G.

History

Deposition	Mar 15, 2026	Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0	Mar 25, 2026	Provider: repository / Type: Initial release

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	24pi.cif.gz	1008.6 KB	Display	PDBx/mmCIF format
PDB format	pdb24pi.ent.gz	700.7 KB	Display	PDB format
PDBx/mmJSON format	24pi.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/4p/24pi ftp://data.pdbj.org/pub/pdb/validation_reports/4p/24pi	HTTPS FTP

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Related structure data

Related structure data	9zglC C: citing same article (ref.)
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#263 - Nov 2021 Acetohydroxyacid Synthase similarity (1) #153 - Sep 2012 Pyruvate Dehydrogenase Complex similarity (1)

Deposited unit

A: Ancestral acetolactate synthase, catabolic

B: Ancestral acetolactate synthase, catabolic

C: Ancestral acetolactate synthase, catabolic

D: Ancestral acetolactate synthase, catabolic

hetero molecules

247 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author&software

Unit cell

Length a, b, c (Å)	104.383, 126.302, 116.534
Angle α, β, γ (deg.)	90.000, 111.389, 90.000
Int Tables number	4
Space group name H-M	P12₁1
Space group name Hall	P2yb
Symmetry operation	#1: x,y,z #2: -x,y+1/2,-z

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Protein , 1 types, 4 molecules ABCD

#1: Protein	Ancestral acetolactate synthase, catabolic Mass: 61250.742 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Plasmid: pET-28a(+) / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): Rosetta / References: acetolactate synthase

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Non-polymers , 5 types, 147 molecules

#2: Chemical	ChemComp-MG / MAGNESIUM ION Mass: 24.305 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mg / Feature type: SUBJECT OF INVESTIGATION
#3: Chemical	ChemComp-TPP / THIAMINE DIPHOSPHATE Mass: 425.314 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Formula: C₁₂H₁₉N₄O₇P₂S / Feature type: SUBJECT OF INVESTIGATION
#4: Chemical	ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL Mass: 92.094 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C₃H₈O₃
#5: Chemical	ChemComp-ACT / ACETATE ION Mass: 59.044 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₂H₃O₂
#6: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 133 / Source method: isolated from a natural source / Formula: H₂O

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Details

Has ligand of interest	Y
Has protein modification	N

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.92 Å³/Da / Density % sol: 57.87 %
Crystal grow	Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 0.1M Tris.HCl, 30% (w/v) MPEG2000, 1 mM NADH, 10 mM MgCl2

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX2 / Wavelength: 0.95372 Å
Detector	Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Nov 2, 2022
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.95372 Å / Relative weight: 1
Reflection	Resolution: 2.75→48.07 Å / Num. obs: 71762 / % possible obs: 98.1 % / Redundancy: 5.4 % / B_iso Wilson estimate: 53.44 Å² / CC_1/2: 0.937 / R_merge(I) obs: 0.193 / R_pim(I) all: 0.141 / R_rim(I) all: 0.24 / Χ²: 0.96 / Net I/σ(I): 6.9
Reflection shell	Resolution: 2.75→2.81 Å / Redundancy: 5.3 % / R_merge(I) obs: 1.279 / Mean I/σ(I) obs: 1.2 / Num. unique obs: 4607 / CC_1/2: 0.45 / R_pim(I) all: 0.915 / R_rim(I) all: 1.582 / Χ²: 0.88 / % possible all: 97.8

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Processing

Software

Name	Version	Classification
XDS		data reduction
Aimless	0.8.2	data scaling
PHASER	2.8.3	phasing
PHENIX	2.0_5936	refinement
Coot	0.9.8.95	model building

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 2.75→45.36 Å / SU ML: 0.3421 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 24.871
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection
R_free	0.2301	3619	5.05 %
R_work	0.2	67993	-
obs	0.2016	71612	97.82 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 60.82 Å²

Refinement step

Cycle: LAST / Resolution: 2.75→45.36 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	16690	0	136	133	16959

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0031	17146
X-RAY DIFFRACTION	f_angle_d	0.5505	23325
X-RAY DIFFRACTION	f_chiral_restr	0.0446	2682
X-RAY DIFFRACTION	f_plane_restr	0.0047	3053
X-RAY DIFFRACTION	f_dihedral_angle_d	15.6678	6221

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.75-2.79	0.3362	144	0.2981	2598	X-RAY DIFFRACTION	97.68
2.79-2.82	0.3368	124	0.2803	2625	X-RAY DIFFRACTION	97.52
2.82-2.86	0.3191	144	0.2818	2590	X-RAY DIFFRACTION	97.57
2.86-2.91	0.3016	145	0.2794	2624	X-RAY DIFFRACTION	97.67
2.91-2.95	0.3206	137	0.2883	2514	X-RAY DIFFRACTION	96.72
2.95-3	0.2888	113	0.2854	2477	X-RAY DIFFRACTION	91.36
3-3.05	0.3212	145	0.2874	2512	X-RAY DIFFRACTION	95.37
3.05-3.11	0.3521	149	0.2661	2614	X-RAY DIFFRACTION	98.78
3.11-3.17	0.2977	116	0.2588	2679	X-RAY DIFFRACTION	98.8
3.17-3.23	0.3104	142	0.2464	2622	X-RAY DIFFRACTION	98.75
3.23-3.3	0.2869	145	0.237	2631	X-RAY DIFFRACTION	98.83
3.3-3.38	0.2578	138	0.2275	2632	X-RAY DIFFRACTION	98.79
3.38-3.46	0.3319	131	0.2833	2608	X-RAY DIFFRACTION	97.86
3.46-3.56	0.2331	124	0.2156	2638	X-RAY DIFFRACTION	98.78
3.56-3.66	0.2743	151	0.2275	2622	X-RAY DIFFRACTION	98.44
3.66-3.78	0.2712	126	0.2082	2654	X-RAY DIFFRACTION	98.55
3.78-3.92	0.2529	134	0.2322	2610	X-RAY DIFFRACTION	97.72
3.92-4.07	0.2175	151	0.1824	2621	X-RAY DIFFRACTION	98.37
4.07-4.26	0.2037	148	0.1619	2596	X-RAY DIFFRACTION	98.6
4.26-4.48	0.161	141	0.1516	2664	X-RAY DIFFRACTION	98.66
4.48-4.76	0.1817	151	0.1464	2623	X-RAY DIFFRACTION	98.61
4.76-5.13	0.1919	127	0.16	2648	X-RAY DIFFRACTION	98.47
5.13-5.65	0.2012	148	0.164	2604	X-RAY DIFFRACTION	97.11
5.65-6.46	0.1919	138	0.1755	2567	X-RAY DIFFRACTION	95.41
6.46-8.13	0.1784	149	0.1625	2697	X-RAY DIFFRACTION	99.65
8.13-45.36	0.1571	158	0.1418	2723	X-RAY DIFFRACTION	99.17

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	3.1918558472545	-0.76690396910483	0.22645218319209	2.4583339225369	0.175683930834	1.6121445377643	0.025138784502174	0.094791365620739	-0.30282121903856	0.20316675675518	0.11699493920924	0.020437997468639	0.08675973134816	0.0014668672779027	-0.13580450457918	0.2755046586865	-0.04832209949729	0.019346934739113	0.202734856337	0.012845975446868	0.25277017752996	-5.466117746767	-21.357419033465	48.215056313617
2	1.7531036408584	-0.20490252699141	0.72873483133463	4.1867183990084	-0.64917858756995	0.84578475874749	-0.15102189787422	0.22409573191376	0.55141279386888	0.18126992616393	-0.017604301771551	-0.18077148522763	-0.26666515595371	0.1337772494543	0.12384311216903	0.37448599280356	-0.014705881516626	0.052527518864725	0.35723808070938	0.010470120407935	0.38781340050169	10.387369631965	6.3394693907356	41.99189551699
3	1.7311104921895	0.010256508128093	-0.13533211094061	1.3116102982718	0.23605899623058	0.54316957508255	-0.026527534241825	0.0016723137010581	0.38513843866323	0.14798614655856	-0.096429947621469	-0.236063925402	-0.15922694500546	0.051069960136657	0.069211856466195	0.47050455163056	-0.056313340876349	-0.00060935887297742	0.27700907199109	0.073678810925716	0.40673569880654	9.9005263310929	13.996041698058	40.766638675221
4	1.8440281192234	-0.069184697743725	0.30480892955674	4.4198986421504	-1.4443181284493	0.62672776808311	-0.18070416512118	0.047260751213746	0.42026931928506	0.3574123581706	0.12365370788198	0.061948294000511	-0.54628266754295	0.066736433989251	0.05807638473591	0.46342129353027	-0.029400827187159	0.0022935041724427	0.33568843881359	0.018299867629892	0.3989795776906	-10.757139974331	8.2342243052609	46.524010866109
5	2.3860122777287	0.19926611829412	0.6825438490919	2.8795754825695	0.81773818221849	2.2298094191476	0.044380974783005	0.42409684015165	0.36280434601694	-0.31357153945837	0.012482672403962	0.28246699746626	-0.35947181137607	-0.017618904722699	-0.1011919189436	0.40106349373494	0.020079565339526	0.01597325993575	0.29597871795222	0.065782075994055	0.51687379067308	-22.160667934623	3.572724073446	41.088751573237
6	2.1282861703675	-0.56707392489172	-1.059304014601	4.3026577754293	-1.3889970330469	8.1729948147721	-0.052714718504499	0.12718948998258	0.25285551175271	-0.01471338122167	-0.11313101682816	0.20492258043807	-0.6358201423291	0.36534976109388	0.092244889233933	0.30230934582681	-0.0028043684007577	-0.082829051732875	0.38766270231652	0.041803882654388	0.52241628608286	-17.240615976548	15.535324827407	43.120880797299
7	2.9200966264509	0.091022227542156	-0.38799867085842	1.8199355175678	0.26053545954075	1.0550373898068	0.05911838261419	0.73611561246755	0.029778833725896	-0.22233673462913	0.092205879911923	0.1496073499783	-0.1371061170555	0.10974060099088	-0.13710218083946	0.28875070568278	-0.024515375207136	-0.012593437610635	0.43931320706378	0.042826709784561	0.28730218317287	-15.117116702258	-12.26634969678	20.552740115559
8	1.1625411112329	-0.25415889709014	0.30282973326264	0.33782747126098	0.18214714894228	1.0618229628582	0.111342686302	0.53314754847678	-0.7046103481161	-0.099970475575612	-0.10459689020416	-0.061254313762239	0.21915148663993	-0.041028519035681	0.028024404336669	0.45740059743853	-0.0030639417692818	-0.1050793674501	0.64272109674676	-0.23906241548683	0.76137968982082	-24.85122983824	-37.357478247177	16.209459257803
9	2.2950545446082	0.083380765792035	-0.69494907689254	2.0015257108472	1.2416970309369	2.5328980644309	0.084641501733956	0.10599104114778	-0.52068508635556	-0.029745884683703	0.012186480027316	0.71026841622113	0.2523723850053	-0.24635254165936	-0.16272440672906	0.38626602554547	-0.056564656384945	-0.11230885497627	0.42630679396751	-0.042076375869215	0.84351875736676	-29.768380658684	-43.101248740799	30.509189463483
10	1.7778134196046	0.24389327610887	0.073281004795872	1.6548571490821	0.51913663181348	1.2357209597671	0.18160948586996	0.065563377658958	-0.47880992412003	0.067302673576212	0.11965546744182	0.23342112956197	0.32381111883385	-0.26828296940661	-0.23131710242671	0.36459415978919	-0.072960734231975	-0.076941113957759	0.42190822441169	0.060809802317729	0.55834224626382	-37.868346858375	-31.938805877217	35.104794407247
11	5.554602636394	1.889874685783	0.20183487947732	0.87527142232565	0.71256734784208	2.0497476517156	0.26198248773884	-0.44079454230408	-0.51480304069967	0.25511291538032	-0.10732831919899	-0.032474891930099	0.35605195161286	-0.033616868998802	-0.080925725205159	0.32978175906679	-0.011225779957443	0.030788273915519	0.32823662809492	0.019224836321844	0.43593601603408	-29.964623206504	-17.122615243207	43.650144811746
12	2.0248680332001	1.3605369991351	0.73610224349803	5.4027665679381	-2.0734433556486	2.8613173871998	0.32340166535904	-0.18681417865451	-0.13628284476491	0.18687891934238	-0.26651350441362	0.017295284068837	0.20171339929902	-0.1671527358477	-0.094017653633871	0.32695681227682	0.0092071543881122	0.10791726570148	0.38004571487761	-0.013927623337723	0.38565763927537	-37.958206477782	-19.258051662452	48.768911378615
13	2.4698809746951	-0.3754070093706	-0.030637365938924	1.3357451969858	0.19357724961544	2.865258847404	0.11634405214883	0.67296394746527	0.084380519872962	-0.20579873802689	-0.036127502362195	-0.09738591870006	-0.034712388500398	0.29666089123707	-0.10769321304684	0.32193110057471	0.0011761424652716	0.022095628873272	0.58047446130366	-0.039946565966223	0.31345799696295	16.574172102816	-17.010194570822	17.96863000001
14	2.0740400387644	-1.0248861464473	2.2944265530763	1.1168664197334	-1.2973061645379	3.2044359798802	-0.0098589328514619	0.2756429620823	0.29963789876965	0.28002189918294	0.054565352013601	0.0028513793678599	-0.41727189848222	0.58838424037153	-0.0060831347681962	0.39061940720025	-0.034882611461853	-0.012147846695143	0.41271948660445	-0.060029236731393	0.55394141744783	24.945425649074	-12.104300084733	43.391187425814
15	5.4941636922334	-1.5239684369769	-0.067171192770763	4.1157136084101	0.18142682237027	3.6183777786564	0.0016306934713563	0.25970413991109	-0.22542717753725	0.1103934521746	0.13137054732183	-0.49005170812708	0.063544100034179	0.37095759150032	-0.073305669902505	0.28296281206556	0.039448395611151	-0.041965575513101	0.31742448709734	-0.059546822715966	0.41829525361868	32.305823574309	-27.188559696964	49.14053500178
16	4.330498978628	-2.6920719611651	-1.0384314950678	3.3663496751584	-0.95900627745893	2.1825348597044	-0.18468194163175	-0.077981703344413	-0.063265383792676	0.089050873683016	0.12808203710337	-0.31573895428787	0.062776870557798	0.072057190535198	0.12323007400681	0.3340254370462	-0.03973846832901	-0.045718547191188	0.31458706531299	-0.056132507911564	0.31029804812971	24.40113543148	-20.521278677457	53.77510581017
17	2.4640492298022	0.02874042585207	0.37433277616362	1.1733644520066	0.22905001054742	4.3105960203238	-0.10043596585892	0.5953833882812	-0.41405012769654	0.067740919086064	0.048805957784093	-0.47623869015561	0.31359697299204	0.58729593052756	0.029831054359662	0.37486211494718	0.025095747248323	-0.021410424068797	0.46493910740084	-0.088154326446929	0.66562041373288	43.653260833608	-41.590898277081	42.679841132105
18	2.8925507433948	-1.9549237996152	-0.053477979862231	2.1702890477135	-1.0505616500352	1.5659593278468	0.37167538937472	0.39232264203611	-0.34736634122816	0.08806837129303	-0.47194928698851	0.097408472788835	-0.05211710522933	0.043911881708982	0.13677832583004	0.35107474140718	-0.0078407033857117	-0.051087292086483	0.43297741710884	-0.18074324074551	0.46821918764061	32.764136057259	-33.804967707969	34.829383523996
19	1.7853693799226	-1.1526151863688	0.31621978156071	2.165201403926	-0.63213396622852	0.95855303371626	0.21468010964537	0.47471694961166	-0.50681428221076	-0.18495566522909	-0.14164626667963	0.097259413279369	0.24179797115971	0.27323825535698	-0.13113079277738	0.35809983281185	0.038799623847412	-0.033869508539209	0.66185261642638	-0.24275249712134	0.53473863275884	29.96053798028	-39.433074012402	24.667169712437
20	2.1945888308826	-0.28781898711286	0.14959445950995	1.4369997920537	-1.143449713693	1.7690284243956	0.30862018603141	0.66069574851019	-0.55060168632559	0.016412932794248	-0.18067437633733	-0.21661747668934	0.239305598684	0.3385351038545	-0.043074611376552	0.45940838119201	0.050733979182917	0.081812453120457	0.60072598617407	-0.33551517838894	0.71616603377146	40.153602256857	-44.737740307577	27.887597579169
21	1.7593076645465	-0.40732436682538	-0.46556996609542	0.53941908626723	-0.18649854646648	2.5946903829662	0.11756107363432	0.35986391347015	-0.56927917321289	-0.11425050155376	-0.022505504304003	0.040347216109959	0.27627707395878	-0.044587217532051	-0.078006822138371	0.4256093054236	0.043408512013767	-0.088702640693265	0.40134398031219	-0.21424732057168	0.59586320989202	4.5930357224845	-43.648094005979	27.499188004373
22	1.0991007046676	-0.0076690813920977	0.49791437247104	0.30980540849793	0.30116672253076	1.0447429301133	0.28466263204828	0.7937290131995	-0.52912661108904	-0.17339403099792	-0.11308466164574	0.18256137704983	0.39030901158978	0.28322739749492	-0.21501474677718	0.61037284865078	0.11444700106775	-0.16907915935324	0.9476486324312	-0.40998402971518	0.84206244922918	-13.767877150981	-42.664528570718	7.18937741894
23	0.89458310433088	-0.30675526068564	-0.5480324872292	1.0645465080419	1.3616750897325	2.310397148425	0.17687736841732	1.4274149761978	-0.45939291096711	-0.41848661387658	0.017416427313772	0.097241833178617	0.19118256838632	0.055558301458669	-0.055813130276995	0.6376075811256	0.15551540235331	-0.11871451127279	1.2355628531404	-0.41043449646945	0.62315660643654	-9.4203298584329	-34.685542375447	-5.0559367142461
24	0.50798919596223	-0.070130146870714	-0.062246553035083	0.58408882347144	0.39450372132107	1.7447448275818	0.11998261343284	0.90563027150051	-0.36838501353731	-0.37458834629254	-0.061825903080054	-0.0910152341793	0.11580546059069	0.021826527931169	-0.10144440965042	0.69907423950729	0.16395986421838	-0.042490148551813	1.3336239287117	-0.49839556050493	0.65159288478798	10.274727253942	-42.285583616067	-6.1449100557373
25	0.64226678591818	0.4844479701124	-0.18588284376551	1.2540232695641	-0.75810887029819	2.2917588231662	0.10794993333937	0.80337119862847	-0.11664448025199	-0.11541676905176	0.014677652012023	-0.083811648537283	0.10002192059284	0.32933142343442	-0.13664785630051	0.54479457960595	0.14048800011697	0.010924696826723	1.0425463920911	-0.384053085569	0.63324636462829	21.953812291273	-39.112959247198	-0.53537870171458

Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

ID	Refine TLS-ID	Selection details	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
1	1	chain 'A' and (resid 6 through 173 )	A	A	6 - 173	1 - 168
2	2	chain 'A' and (resid 174 through 304 )	A	A	174 - 304	169 - 299
3	3	chain 'A' and (resid 305 through 365 )	A	A	305 - 365	300 - 360
4	4	chain 'A' and (resid 366 through 440 )	A	A	366 - 440	361 - 435
5	5	chain 'A' and (resid 441 through 520 )	A	A	441 - 520	436 - 515
6	6	chain 'A' and (resid 521 through 559 )	A	A	521 - 559	516 - 554
7	7	chain 'B' and (resid 6 through 173 )	B	F	6 - 173	1 - 168
8	8	chain 'B' and (resid 174 through 220 )	B	F	174 - 220	169 - 215
9	9	chain 'B' and (resid 221 through 317 )	B	F	221 - 317	216 - 312
10	10	chain 'B' and (resid 318 through 440 )	B	F	318 - 440	313 - 435
11	11	chain 'B' and (resid 441 through 488 )	B	F	441 - 488	436 - 483
12	12	chain 'B' and (resid 489 through 559 )	B	F	489 - 559	484 - 554
13	13	chain 'C' and (resid 6 through 173 )	C	J	6 - 173	1 - 168
14	14	chain 'C' and (resid 174 through 220 )	C	J	174 - 220	169 - 215
15	15	chain 'C' and (resid 221 through 289 )	C	J	221 - 289	216 - 284
16	16	chain 'C' and (resid 290 through 342 )	C	J	290 - 342	285 - 337
17	17	chain 'C' and (resid 343 through 386 )	C	J	343 - 386	338 - 381
18	18	chain 'C' and (resid 387 through 423 )	C	J	387 - 423	382 - 418
19	19	chain 'C' and (resid 424 through 500 )	C	J	424 - 500	419 - 495
20	20	chain 'C' and (resid 501 through 559 )	C	J	501 - 559	496 - 554
21	21	chain 'D' and (resid 6 through 173 )	D	O	6 - 173	1 - 168
22	22	chain 'D' and (resid 174 through 226 )	D	O	174 - 226	169 - 221
23	23	chain 'D' and (resid 227 through 342 )	D	O	227 - 342	222 - 337
24	24	chain 'D' and (resid 343 through 440 )	D	O	343 - 440	338 - 435
25	25	chain 'D' and (resid 441 through 559 )	D	O	441 - 559	436 - 554

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