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Open data
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Basic information
Entry | Database: PDB / ID: 1zxg | ||||||
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Title | Solution structure of A219 | ||||||
![]() | Immunoglobulin G binding protein A | ||||||
![]() | Immune System/Protein Binding / IgG-binding / protein A / phage display / Immune System-Protein Binding COMPLEX | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / CNS 1.1. | ||||||
![]() | He, Y. / Yeh, D.C. / Alexander, P. / Bryan, P.N. / Orban, J. | ||||||
![]() | ![]() Title: Solution NMR structures of IgG binding domains with artificially evolved high levels of sequence identity but different folds. Authors: He, Y. / Yeh, D.C. / Alexander, P. / Bryan, P.N. / Orban, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 364.3 KB | Display | ![]() |
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PDB format | ![]() | 304.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 341.5 KB | Display | ![]() |
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Full document | ![]() | 474.7 KB | Display | |
Data in XML | ![]() | 31.5 KB | Display | |
Data in CIF | ![]() | 47.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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NMR ensembles |
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Components
#1: Antibody | Mass: 6733.484 Da / Num. of mol.: 1 / Mutation: yes Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: A219 mutant protein, 2 mM ammonium bicarbonate buffer, pH 7.0, 10% D2O Solvent system: 2 mM ammonium bicarbonate buffer, 10% D2O. |
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Sample conditions | Ionic strength: 2 mM / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: CNS 1.1. / Software ordinal: 1 / Details: simulated annealing. | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: The submitted conformer models are those with the fewest number of constraint violations. Conformers calculated total number: 50 / Conformers submitted total number: 20 |