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- PDB-1zug: STRUCTURE OF PHAGE 434 CRO PROTEIN, NMR, 20 STRUCTURES -

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Basic information

Entry
Database: PDB / ID: 1zug
TitleSTRUCTURE OF PHAGE 434 CRO PROTEIN, NMR, 20 STRUCTURES
ComponentsPHAGE 434 CRO PROTEIN
KeywordsTRANSCRIPTION REGULATION / GENE REGULATING PROTEIN
Function / homology
Function and homology information


regulation of DNA-templated transcription / DNA binding
Similarity search - Function
Regulatory protein cro / Helix-turn-helix / Helix-turn-helix XRE-family like proteins / Cro/C1-type HTH domain profile. / lambda repressor-like DNA-binding domains / Cro/C1-type helix-turn-helix domain / 434 Repressor (Amino-terminal Domain) / Lambda repressor-like, DNA-binding domain superfamily / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Regulatory protein cro
Similarity search - Component
Biological speciesPhage 434 (virus)
MethodSOLUTION NMR / DISTANCE GEOMETRY MOLECULAR DYNAMICS
AuthorsPadmanabhan, S. / Jimenez, M.A. / Gonzalez, C. / Sanz, J.M. / Gimenez-Gallego, G. / Rico, M.
CitationJournal: Biochemistry / Year: 1997
Title: Three-dimensional solution structure and stability of phage 434 Cro protein.
Authors: Padmanabhan, S. / Jimenez, M.A. / Gonzalez, C. / Sanz, J.M. / Gimenez-Gallego, G. / Rico, M.
History
DepositionMar 14, 1997Processing site: BNL
Revision 1.0Jul 7, 1997Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Mar 2, 2022Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site
Revision 1.4May 22, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: PHAGE 434 CRO PROTEIN


Theoretical massNumber of molelcules
Total (without water)8,0771
Polymers8,0771
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 20
Representative

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Components

#1: Protein PHAGE 434 CRO PROTEIN


Mass: 8076.619 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Phage 434 (virus) / Genus: Lambda-like viruses / Species: Enterobacteria phage lambda
Description: THE PLASMID WAS A GIFT FROM THE LABORATORY OF PROFESSOR STEVE HARRISON (HARVARD UNIVERSITY)
Plasmid: PRW74 / Production host: Escherichia coli (E. coli) / References: UniProt: P03036

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
111NOESY
121TOCSY
131COSY

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Sample preparation

Sample conditionspH: 6.0 / Temperature: 293 K
Crystal grow
*PLUS
Method: other / Details: NMR

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NMR measurement

NMR spectrometerType: Bruker AMX 600 / Manufacturer: Bruker / Model: AMX 600 / Field strength: 600 MHz

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Processing

NMR software
NameVersionDeveloperClassification
GROMOSBERENDSENrefinement
DIANA, GROMOSGROMOSstructure solution
RefinementMethod: DISTANCE GEOMETRY MOLECULAR DYNAMICS / Software ordinal: 1
NMR ensembleConformers calculated total number: 20 / Conformers submitted total number: 20

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