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Yorodumi- PDB-1zsx: Crystal Structure Of Human Potassium Channel Kv Beta-subunit (KCNAB2) -
+Open data
-Basic information
Entry | Database: PDB / ID: 1zsx | ||||||
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Title | Crystal Structure Of Human Potassium Channel Kv Beta-subunit (KCNAB2) | ||||||
Components | Voltage-gated potassium channel beta-2 subunit | ||||||
Keywords | OXIDOREDUCTASE / Potassium Channel / Kv Beta-subunit / human / aldo-keto reductase / Structural Genomics / SGC / Structural Genomics Consortium | ||||||
Function / homology | Function and homology information pinceau fiber / regulation of action potential / NADPH oxidation / regulation of protein localization to cell surface / aldo-keto reductase (NADPH) activity / Voltage gated Potassium channels / juxtaparanode region of axon / regulation of potassium ion transmembrane transport / Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor / voltage-gated potassium channel activity ...pinceau fiber / regulation of action potential / NADPH oxidation / regulation of protein localization to cell surface / aldo-keto reductase (NADPH) activity / Voltage gated Potassium channels / juxtaparanode region of axon / regulation of potassium ion transmembrane transport / Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor / voltage-gated potassium channel activity / tertiary granule membrane / potassium channel regulator activity / specific granule membrane / voltage-gated potassium channel complex / extrinsic component of cytoplasmic side of plasma membrane / cytoplasmic side of plasma membrane / transmembrane transporter binding / cytoskeleton / synapse / Neutrophil degranulation / membrane / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.9 Å | ||||||
Authors | Wennerstrand, P. / Johannsson, I. / Ugochukwu, E. / Kavanagh, K. / Edwards, A. / Arrowsmith, C. / Williams, L. / Sundstrom, M. / Guo, K. / von Delft, F. ...Wennerstrand, P. / Johannsson, I. / Ugochukwu, E. / Kavanagh, K. / Edwards, A. / Arrowsmith, C. / Williams, L. / Sundstrom, M. / Guo, K. / von Delft, F. / Oppermann, U. / Structural Genomics Consortium (SGC) | ||||||
Citation | Journal: To be Published Title: Crystal Structure Of Human Potassium Channel Kv Beta-subunit (KCNAB2) Authors: Wennerstrand, P. / Johannsson, I. / Ugochukwu, E. / Kavanagh, K. / Edwards, A. / Arrowsmith, C. / Williams, L. / Sundstrom, M. / Guo, K. / von Delft, F. / Oppermann, U. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1zsx.cif.gz | 85.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1zsx.ent.gz | 62.6 KB | Display | PDB format |
PDBx/mmJSON format | 1zsx.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zs/1zsx ftp://data.pdbj.org/pub/pdb/validation_reports/zs/1zsx | HTTPS FTP |
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-Related structure data
Related structure data | 1qrqS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 38901.590 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: Origene / Plasmid: p11 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21-DE3 / References: UniProt: Q13303 | ||||
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#2: Chemical | ChemComp-CL / #3: Chemical | ChemComp-NAP / | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.9 Å3/Da / Density % sol: 57.7 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.2 Details: PEG300, sodium/potassium phosphate, sodium chloride, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X10SA / Wavelength: 0.9791 Å |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Apr 23, 2005 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→44.3 Å / Num. obs: 36582 / % possible obs: 99.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
Reflection shell | Resolution: 1.9→2 Å / % possible all: 99 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1QRQ.pdb Resolution: 1.9→44.3 Å / Cor.coef. Fo:Fc: 0.972 / Cor.coef. Fo:Fc free: 0.963 / SU B: 4.821 / SU ML: 0.072 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.096 / ESU R Free: 0.094 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 26.597 Å2
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Refinement step | Cycle: LAST / Resolution: 1.9→44.3 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.9→1.949 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Origin x: 7.0447 Å / Origin y: -30.3171 Å / Origin z: 19.233 Å
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