+Open data
-Basic information
Entry | Database: PDB / ID: 1zrw | ||||||
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Title | solution structure of spinigerin in H20/TFE 10% | ||||||
Components | spinigerin | ||||||
Keywords | ANTIMICROBIAL PROTEIN / ANTIBIOTIC / helical peptide in TFE | ||||||
Function / homology | defense response to fungus / killing of cells of another organism / defense response to bacterium / extracellular region / Spinigerin Function and homology information | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
Authors | Landon, C. / Meudal, H. / Boulanger, N. / Bulet, P. / Vovelle, F. | ||||||
Citation | Journal: Biopolymers / Year: 2006 Title: Solution structures of stomoxyn and spinigerin, two insect antimicrobial peptides with an alpha-helical conformation. Authors: Landon, C. / Meudal, H. / Boulanger, N. / Bulet, P. / Vovelle, F. #1: Journal: J.Biol.Chem. / Year: 2001 Title: Insect immunity. Constitutive expression of a cysteine-rich antifungal and a linear antibacterial peptide in a termite insect Authors: Lamberty, M. / Zachary, D. / Lanot, R. / Bordereau, C. / Robert, A. / Hoffmann, J.A. / Bulet, P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1zrw.cif.gz | 302.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1zrw.ent.gz | 255.6 KB | Display | PDB format |
PDBx/mmJSON format | 1zrw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1zrw_validation.pdf.gz | 340.1 KB | Display | wwPDB validaton report |
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Full document | 1zrw_full_validation.pdf.gz | 525.8 KB | Display | |
Data in XML | 1zrw_validation.xml.gz | 16.6 KB | Display | |
Data in CIF | 1zrw_validation.cif.gz | 25.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zr/1zrw ftp://data.pdbj.org/pub/pdb/validation_reports/zr/1zrw | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 3008.799 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: solid-phase synthesis (Fmoc). Sequence occurs naturally in Pseudacanthotermes spiniger References: UniProt: P82357 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques. |
-Sample preparation
Details | Contents: 1.5mM spinigerin / Solvent system: 90% H2O, 10% TFE |
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Sample conditions | pH: 5.7 / Pressure: ambiant / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: molecular dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: representative conformer | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 30 |