+Open data
-Basic information
Entry | Database: PDB / ID: 1z23 | ||||||
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Title | The serine-rich domain from Crk-associated substrate (p130Cas) | ||||||
Components | CRK-associated substrate | ||||||
Keywords | CELL ADHESION / four-helix bundle | ||||||
Function / homology | Function and homology information p130Cas linkage to MAPK signaling for integrins / PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases / VEGFA-VEGFR2 Pathway / Downstream signal transduction / antigen receptor-mediated signaling pathway / cellular response to endothelin / negative regulation of substrate adhesion-dependent cell spreading / endothelin receptor signaling pathway / hepatocyte growth factor receptor signaling pathway / cellular response to hepatocyte growth factor stimulus ...p130Cas linkage to MAPK signaling for integrins / PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases / VEGFA-VEGFR2 Pathway / Downstream signal transduction / antigen receptor-mediated signaling pathway / cellular response to endothelin / negative regulation of substrate adhesion-dependent cell spreading / endothelin receptor signaling pathway / hepatocyte growth factor receptor signaling pathway / cellular response to hepatocyte growth factor stimulus / neurotrophin TRK receptor signaling pathway / platelet-derived growth factor receptor signaling pathway / cellular response to nitric oxide / vascular endothelial growth factor receptor signaling pathway / ruffle / cell surface receptor protein tyrosine kinase signaling pathway / positive regulation of endothelial cell migration / cell chemotaxis / integrin-mediated signaling pathway / actin filament organization / B cell receptor signaling pathway / epidermal growth factor receptor signaling pathway / SH3 domain binding / cell migration / actin cytoskeleton / insulin receptor signaling pathway / lamellipodium / actin cytoskeleton organization / cell adhesion / positive regulation of cell migration / G protein-coupled receptor signaling pathway / protein domain specific binding / axon / focal adhesion / protein kinase binding / membrane / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Briknarova, K. / Nasertorabi, F. / Havert, M.L. / Eggleston, E. / Hoyt, D.W. / Li, C. / Olson, A.J. / Vuori, K. / Ely, K.R. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2005 Title: The serine-rich domain from Crk-associated substrate (p130cas) is a four-helix bundle. Authors: Briknarova, K. / Nasertorabi, F. / Havert, M.L. / Eggleston, E. / Hoyt, D.W. / Li, C. / Olson, A.J. / Vuori, K. / Ely, K.R. #1: Journal: J.Biol.Chem. / Year: 1999 Title: Cell adhesion regulates the interactions between the docking protein p130(Cas) and the 14-3-3 proteins Authors: Garcia-Guzman, M. / Dolfi, F. / Russello, M. / Vuori, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1z23.cif.gz | 946.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1z23.ent.gz | 796.1 KB | Display | PDB format |
PDBx/mmJSON format | 1z23.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1z23_validation.pdf.gz | 345.9 KB | Display | wwPDB validaton report |
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Full document | 1z23_full_validation.pdf.gz | 523.6 KB | Display | |
Data in XML | 1z23_validation.xml.gz | 68.6 KB | Display | |
Data in CIF | 1z23_validation.cif.gz | 90.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/z2/1z23 ftp://data.pdbj.org/pub/pdb/validation_reports/z2/1z23 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 17348.465 Da / Num. of mol.: 1 / Fragment: serine-rich domain, SRR-B, residues 546-708 / Mutation: V546G P547S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q63767 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions | Ionic strength: 0.1 / pH: 7.9 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | ||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 Details: The calculation employed a total of 1562 unambiguous distance restraints (166 long range, 245 medium range, 346 sequential and 805 intraresidual), 6 ambiguous long-range distance restraints, ...Details: The calculation employed a total of 1562 unambiguous distance restraints (166 long range, 245 medium range, 346 sequential and 805 intraresidual), 6 ambiguous long-range distance restraints, 176 hydrogen bond restraints, 107 phi and 107 psi dihedral angle restraints, and 79 residual dipolar coupling restraints. | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 99 / Conformers submitted total number: 20 |