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- PDB-1ymo: Solution structure of the P2b-P3 pseudoknot from human telomerase RNA -
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Open data
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Basic information
Entry | Database: PDB / ID: 1ymo | ||||||
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Title | Solution structure of the P2b-P3 pseudoknot from human telomerase RNA | ||||||
![]() | Telomerase RNA P2b-P3 pseudoknot | ||||||
![]() | RNA / pseudoknot / non-canonical / tertiary structure / Hoogsteen / triplex | ||||||
Function / homology | RNA / RNA (> 10)![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Theimer, C.A. / Blois, C.A. / Feigon, J. | ||||||
![]() | ![]() Title: Structure of the human telomerase RNA pseudoknot reveals conserved tertiary interactions essential for function Authors: Theimer, C.A. / Blois, C.A. / Feigon, J. #1: ![]() Title: Mutations linked to dyskeratosis congenita cause changes in the structural equilibrium in telomerase RNA Authors: Theimer, C.A. / Finger, L.D. / Trantirek, L. / Feigon, J. #2: ![]() Title: YNMG tetraloop formation by a dyskeratosis congenita mutation in human telomerase RNA Authors: Theimer, C.A. / Finger, L.D. / Feigon, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 573.6 KB | Display | ![]() |
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PDB format | ![]() | 486.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 322.7 KB | Display | ![]() |
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Full document | ![]() | 499.5 KB | Display | |
Data in XML | ![]() | 59.2 KB | Display | |
Data in CIF | ![]() | 85.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: RNA chain | Mass: 14982.920 Da / Num. of mol.: 1 / Fragment: P2b-P3 pseudoknot / Mutation: U177 deletion / Source method: obtained synthetically Details: RNA is synthesized using in vitro transcription with T7 RNA polymerase and a partially double-stranded DNA template. The sequence of this RNA can be found naturally in homo sapiens (human). |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: The same experiments were performed on all of the singly-labeled samples except for residual dipolar couplings which were only collected for the A- and G-only labeled samples. |
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Sample preparation
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Sample conditions |
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-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | ||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: Structures are based on 790 NOE-derived distance constraints, 210 dihedral angle restraints, 50 distance restraints from hydrogen bonds, and 20 H-C residual dipolar couplings. PDB entry PDB ...Details: Structures are based on 790 NOE-derived distance constraints, 210 dihedral angle restraints, 50 distance restraints from hydrogen bonds, and 20 H-C residual dipolar couplings. PDB entry PDB entry 2k96 is a better refined structure of entry 1ymo. | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy, no restraint violations, consistent with residual dipolar couplings Conformers calculated total number: 200 / Conformers submitted total number: 20 |