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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1y6w | ||||||
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タイトル | Trapped intermediate of calmodulin | ||||||
![]() | Calmodulin | ||||||
![]() | Calcium-Binding Protein / EF-hand / engineered disulfide | ||||||
機能・相同性 | ![]() : / establishment of protein localization to mitochondrial membrane / type 3 metabotropic glutamate receptor binding / CaM pathway / Cam-PDE 1 activation / Sodium/Calcium exchangers / regulation of synaptic vesicle endocytosis / Calmodulin induced events / Reduction of cytosolic Ca++ levels / CREB1 phosphorylation through the activation of CaMKII/CaMKK/CaMKIV cascasde ...: / establishment of protein localization to mitochondrial membrane / type 3 metabotropic glutamate receptor binding / CaM pathway / Cam-PDE 1 activation / Sodium/Calcium exchangers / regulation of synaptic vesicle endocytosis / Calmodulin induced events / Reduction of cytosolic Ca++ levels / CREB1 phosphorylation through the activation of CaMKII/CaMKK/CaMKIV cascasde / Activation of Ca-permeable Kainate Receptor / Loss of phosphorylation of MECP2 at T308 / CREB1 phosphorylation through the activation of Adenylate Cyclase / PKA activation / negative regulation of high voltage-gated calcium channel activity / CaMK IV-mediated phosphorylation of CREB / Glycogen breakdown (glycogenolysis) / positive regulation of cyclic-nucleotide phosphodiesterase activity / organelle localization by membrane tethering / negative regulation of calcium ion export across plasma membrane / regulation of synaptic vesicle exocytosis / CLEC7A (Dectin-1) induces NFAT activation / regulation of cardiac muscle cell action potential / mitochondrion-endoplasmic reticulum membrane tethering / autophagosome membrane docking / Activation of RAC1 downstream of NMDARs / response to corticosterone / nitric-oxide synthase binding / positive regulation of ryanodine-sensitive calcium-release channel activity / regulation of cell communication by electrical coupling involved in cardiac conduction / Negative regulation of NMDA receptor-mediated neuronal transmission / negative regulation of peptidyl-threonine phosphorylation / Synthesis of IP3 and IP4 in the cytosol / Unblocking of NMDA receptors, glutamate binding and activation / Phase 0 - rapid depolarisation / protein phosphatase activator activity / RHO GTPases activate PAKs / positive regulation of phosphoprotein phosphatase activity / Ion transport by P-type ATPases / Long-term potentiation / Uptake and function of anthrax toxins / Calcineurin activates NFAT / Regulation of MECP2 expression and activity / adenylate cyclase binding / catalytic complex / DARPP-32 events / detection of calcium ion / regulation of cardiac muscle contraction / negative regulation of ryanodine-sensitive calcium-release channel activity / Smooth Muscle Contraction / RHO GTPases activate IQGAPs / calcium channel inhibitor activity / cellular response to interferon-beta / positive regulation of DNA binding / regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion / Protein methylation / phosphatidylinositol 3-kinase binding / eNOS activation / Activation of AMPK downstream of NMDARs / regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum / enzyme regulator activity / Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation / positive regulation of protein dephosphorylation / Ion homeostasis / regulation of calcium-mediated signaling / regulation of ryanodine-sensitive calcium-release channel activity / titin binding / positive regulation of protein autophosphorylation / voltage-gated potassium channel complex / sperm midpiece / calcium channel complex / response to amphetamine / activation of adenylate cyclase activity / substantia nigra development / adenylate cyclase activator activity / Ras activation upon Ca2+ influx through NMDA receptor / nitric-oxide synthase regulator activity / regulation of heart rate / sarcomere / protein serine/threonine kinase activator activity / FCERI mediated Ca+2 mobilization / FCGR3A-mediated IL10 synthesis / VEGFR2 mediated vascular permeability / positive regulation of peptidyl-threonine phosphorylation / regulation of cytokinesis / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / VEGFR2 mediated cell proliferation / positive regulation of nitric-oxide synthase activity / Translocation of SLC2A4 (GLUT4) to the plasma membrane / mitochondrial membrane / positive regulation of receptor signaling pathway via JAK-STAT / RAF activation / Transcriptional activation of mitochondrial biogenesis / positive regulation of protein serine/threonine kinase activity / spindle microtubule / Stimuli-sensing channels / synaptic vesicle membrane / cellular response to type II interferon / spindle pole / response to calcium ion 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Grabarek, Z. | ||||||
![]() | ![]() タイトル: Structure of a Trapped Intermediate of Calmodulin: Calcium Regulation of EF-hand Proteins from a New Perspective. 著者: Grabarek, Z. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロード
PDBx/mmCIF形式 | ![]() | 42.2 KB | 表示 | ![]() |
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PDB形式 | ![]() | 32.2 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 452.4 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 454.3 KB | 表示 | |
XML形式データ | ![]() | 8.9 KB | 表示 | |
CIF形式データ | ![]() | 11.6 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 17091.398 Da / 分子数: 1 / 変異: Q41C, D64N, K75C / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() | ||||||
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#2: 化合物 | ChemComp-CA / #3: 化合物 | ChemComp-MPD / ( | #4: 化合物 | ChemComp-TBU / | #5: 水 | ChemComp-HOH / | |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 3.8 Å3/Da / 溶媒含有率: 67.2 % |
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結晶化 | 温度: 277 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 5.2 詳細: 32% (w/v) 2-methyl-2,4-pentanediol, 10% (v/v) t-butanol, 20 mM Na-cacodylate, 5 mM CaCl2, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-データ収集
回折 | 平均測定温度: 100 K | ||||||||||||
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放射光源 | 由来: ![]() ![]() ![]() | ||||||||||||
検出器 | タイプ: ADSC QUANTUM 4 / 検出器: CCD / 日付: 2000年6月23日 | ||||||||||||
放射 | プロトコル: MAD / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray | ||||||||||||
放射波長 |
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反射 | 解像度: 2.4→30 Å / Num. obs: 10558 / % possible obs: 98.6 % / 冗長度: 8.8 % / Biso Wilson estimate: 47.9 Å2 / Rmerge(I) obs: 0.064 / Net I/σ(I): 18.9 | ||||||||||||
反射 シェル | 解像度: 2.4→2.49 Å / Rmerge(I) obs: 0.38 / Mean I/σ(I) obs: 3.5 / % possible all: 98.2 |
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解析
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精密化 | 構造決定の手法: ![]() 詳細: The N-terminal domain is locked in a closed conformation with a disulfide bond. This prevents the glutamate in the 12th position of the loop from interacting with the calcium ion in sites I ...詳細: The N-terminal domain is locked in a closed conformation with a disulfide bond. This prevents the glutamate in the 12th position of the loop from interacting with the calcium ion in sites I and II. Bond angles in residues 60, 61, 94 and 132 deviate by more than 6*RMSD relative to the standard dictionary (see REMARK 500). These residues are located in the calcium binding loops, thus their conformation is constrained by the calcium ions.
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溶媒の処理 | 溶媒モデル: FLAT MODEL / Bsol: 38.8873 Å2 / ksol: 0.321444 e/Å3 | ||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 58.2 Å2
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Refine analyze |
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精密化ステップ | サイクル: LAST / 解像度: 2.4→19.88 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 2.4→2.55 Å / Rfactor Rfree error: 0.032 / Total num. of bins used: 6
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Xplor file |
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