[English] 日本語
![](img/lk-miru.gif)
- PDB-1xwm: The crystal structure of PhoU (phosphate uptake regulator), Struc... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1xwm | ||||||
---|---|---|---|---|---|---|---|
Title | The crystal structure of PhoU (phosphate uptake regulator), Structural genomics | ||||||
![]() | phosphate uptake regulator | ||||||
![]() | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / PhoU / Negative phosphate uptake regulator / protein structure initiative / PSI / MCSG / Midwest center for structural genomics | ||||||
Function / homology | Phosphate transport system protein phou homolog 2; domain 2 / Methane Monooxygenase Hydroxylase; Chain G, domain 1 / Up-down Bundle / Mainly Alpha![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhang, R. / Dementieva, I. / Moy, S. / Joachimiak, A. / Midwest Center for Structural Genomics (MCSG) | ||||||
![]() | ![]() Title: The crystal structure of PhoU (phosphate uptake regulator) Authors: Zhang, R. / Dementieva, I. / Moy, S. / Joachimiak, A. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 50.9 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 37.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 420.6 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 431.2 KB | Display | |
Data in XML | ![]() | 10.6 KB | Display | |
Data in CIF | ![]() | 13.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
---|---|
Other databases |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
Unit cell |
| ||||||||
Details | This protein exists as dimer. The second part of the dimer is generated by crystallographic two fold axis. |
-
Components
#1: Protein | Mass: 23804.146 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: RBSTP1653 / Plasmid: PDM68 / Species (production host): Escherichia coli / Production host: ![]() ![]() |
---|---|
#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.29 Å3/Da / Density % sol: 44.22 % |
---|---|
Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.9 Details: 16% PEG3350, 0.2M ammonium tartrate, 0.1M Bis-Tris buffer, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Diffraction source | Source: ![]() ![]() ![]() | |||||||||
Detector | Type: SBC-2 / Detector: CCD / Date: Nov 19, 2003 / Details: mirrors | |||||||||
Radiation | Monochromator: Si 111 channel / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
| |||||||||
Reflection | Resolution: 2.5→50 Å / Num. all: 15341 / Num. obs: 15295 / % possible obs: 99.7 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 8.5 % / Biso Wilson estimate: 23.3 Å2 / Rmerge(I) obs: 0.104 / Net I/σ(I): 22.6 | |||||||||
Reflection shell | Resolution: 2.5→2.59 Å / Redundancy: 7.8 % / Rmerge(I) obs: 0.667 / Mean I/σ(I) obs: 1.87 / Num. unique all: 1524 / % possible all: 98.6 |
-
Processing
Software |
| |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| |||||||||||||||||||||||||
Solvent computation | Solvent model: FLAT MODEL / Bsol: 42.6805 Å2 / ksol: 0.309509 e/Å3 | |||||||||||||||||||||||||
Displacement parameters | Biso mean: 49.3 Å2
| |||||||||||||||||||||||||
Refine analyze |
| |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→30.59 Å
| |||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||
LS refinement shell | Resolution: 2.5→2.66 Å / Rfactor Rfree error: 0.037 / Total num. of bins used: 6
| |||||||||||||||||||||||||
Xplor file |
|