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- PDB-1xpu: Structural mechanism of inhibition of the Rho transcription termi... -

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Basic information

Entry
Database: PDB / ID: 1xpu
TitleStructural mechanism of inhibition of the Rho transcription termination factor by the antibiotic 5a-(3-formylphenylsulfanyl)-dihydrobicyclomycin (FPDB)
Components
  • 5'-R(*CP*UP*CP*UP*CP*UP*CP*U)-3'
  • Rho transcription termination factor
KeywordsTRANSCRIPTION/RNA / Rho transcription termination factor / 5a-(3-formylphenylsulfanyl)-dihydrobicyclomycin (FPDB) / ATPgammaS / TRANSCRIPTION-RNA COMPLEX
Function / homology
Function and homology information


methane monooxygenase (soluble) / : / : / : / ATP-dependent activity, acting on RNA / helicase activity / DNA-templated transcription termination / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / one-carbon metabolic process / ATP hydrolysis activity ...methane monooxygenase (soluble) / : / : / : / ATP-dependent activity, acting on RNA / helicase activity / DNA-templated transcription termination / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / one-carbon metabolic process / ATP hydrolysis activity / RNA binding / ATP binding / identical protein binding / membrane / metal ion binding / cytosol
Similarity search - Function
Transcription termination factor Rho / Rho termination factor, N-terminal / Rho termination factor, RNA-binding domain / Transcription termination factor Rho, ATP binding domain / Rho termination factor, RNA-binding domain / Rho termination factor, N-terminal domain / Rho RNA-binding domain profile. / Rho termination factor, N-terminal domain / Rho termination factor, N-terminal domain superfamily / Propane/methane/phenol/toluene hydroxylase ...Transcription termination factor Rho / Rho termination factor, N-terminal / Rho termination factor, RNA-binding domain / Transcription termination factor Rho, ATP binding domain / Rho termination factor, RNA-binding domain / Rho termination factor, N-terminal domain / Rho RNA-binding domain profile. / Rho termination factor, N-terminal domain / Rho termination factor, N-terminal domain superfamily / Propane/methane/phenol/toluene hydroxylase / Methane/Phenol/Alkene Hydroxylase / Cold shock domain / Cold shock protein domain / Ribonucleotide reductase-like / ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain / ATP synthase alpha/beta family, nucleotide-binding domain / Nucleic acid-binding proteins / Ferritin-like superfamily / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / P-loop containing nucleotide triphosphate hydrolases / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / Nucleic acid-binding, OB-fold / Beta Barrel / Rossmann fold / P-loop containing nucleoside triphosphate hydrolase / 3-Layer(aba) Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / 5A-(3-FORMYLPHENYLSULFANYL)-DIHYDROBICYCLOMYCIN / RNA / Transcription termination factor Rho / Methane monooxygenase component A alpha chain
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.05 Å
AuthorsSkordalakes, E. / Brogan, A.P. / Park, B.S. / Kohn, H. / Berger, J.M.
CitationJournal: Structure / Year: 2005
Title: Structural mechanism of inhibition of the rho transcription termination factor by the antibiotic bicyclomycin
Authors: Skordalakes, E. / Brogan, A.P. / Park, B.S. / Kohn, H. / Berger, J.M.
History
DepositionOct 9, 2004Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 2, 2004Provider: repository / Type: Initial release
Revision 1.1Apr 30, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Advisory / Version format compliance

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
G: 5'-R(*CP*UP*CP*UP*CP*UP*CP*U)-3'
M: 5'-R(*CP*UP*CP*UP*CP*UP*CP*U)-3'
H: 5'-R(*CP*UP*CP*UP*CP*UP*CP*U)-3'
J: 5'-R(*CP*UP*CP*UP*CP*UP*CP*U)-3'
K: 5'-R(*CP*UP*CP*UP*CP*UP*CP*U)-3'
L: 5'-R(*CP*UP*CP*UP*CP*UP*CP*U)-3'
A: Rho transcription termination factor
B: Rho transcription termination factor
C: Rho transcription termination factor
D: Rho transcription termination factor
E: Rho transcription termination factor
F: Rho transcription termination factor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)302,31129
Polymers296,82412
Non-polymers5,48817
Water36020
1
G: 5'-R(*CP*UP*CP*UP*CP*UP*CP*U)-3'
A: Rho transcription termination factor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)50,0184
Polymers49,4712
Non-polymers5482
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
M: 5'-R(*CP*UP*CP*UP*CP*UP*CP*U)-3'
B: Rho transcription termination factor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)50,4595
Polymers49,4712
Non-polymers9883
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
H: 5'-R(*CP*UP*CP*UP*CP*UP*CP*U)-3'
C: Rho transcription termination factor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)50,4595
Polymers49,4712
Non-polymers9883
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
J: 5'-R(*CP*UP*CP*UP*CP*UP*CP*U)-3'
D: Rho transcription termination factor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)50,4595
Polymers49,4712
Non-polymers9883
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
5
K: 5'-R(*CP*UP*CP*UP*CP*UP*CP*U)-3'
E: Rho transcription termination factor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)50,9826
Polymers49,4712
Non-polymers1,5114
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
6
L: 5'-R(*CP*UP*CP*UP*CP*UP*CP*U)-3'
F: Rho transcription termination factor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)49,9354
Polymers49,4712
Non-polymers4652
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)119.477, 206.935, 148.607
Angle α, β, γ (deg.)90.00, 96.95, 90.00
Int Tables number5
Cell settingmonoclinic
Space group name H-MC121

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Components

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RNA chain / Protein , 2 types, 12 molecules GMHJKLABCDEF

#1: RNA chain
5'-R(*CP*UP*CP*UP*CP*UP*CP*U)-3'


Mass: 2400.433 Da / Num. of mol.: 6 / Source method: obtained synthetically / Details: ssRNA
#2: Protein
Rho transcription termination factor


Mass: 47070.168 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) / References: UniProt: P22869, UniProt: P0AG30*PLUS

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Non-polymers , 4 types, 37 molecules

#3: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: Mg
#4: Chemical
ChemComp-AGS / PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-GAMMA-S / ADENOSINE 5'-(3-THIOTRIPHOSPHATE) / ADENOSINE 5'-(GAMMA-THIOTRIPHOSPHATE) / ADENOSINE-5'-DIPHOSPHATE MONOTHIOPHOSPHATE


Mass: 523.247 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C10H16N5O12P3S / Comment: ATP-gamma-S, energy-carrying molecule analogue*YM
#5: Chemical
ChemComp-FPD / 5A-(3-FORMYLPHENYLSULFANYL)-DIHYDROBICYCLOMYCIN


Mass: 440.467 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C19H24N2O8S
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 20 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.16 Å3/Da / Density % sol: 60.8 %
Crystal growTemperature: 293 K / Method: vapor diffusion / pH: 6.5
Details: PEG 8000, Na Cacodylate, NaCl, Glycerol, FOS-Choline-12, pH 6.5, VAPOR DIFFUSION, temperature 293K
Components of the solutions
IDNameCrystal-IDSol-ID
1PEG 800011
2NaCl11
3glycerol11
4FOS-Choline-1211
5sodium Cacodylate11
6PEG 800012
7NaCl12
8glycerol12

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ALS / Beamline: 8.3.1 / Wavelength: 1.1 Å
DetectorDetector: CCD
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.1 Å / Relative weight: 1
ReflectionResolution: 2.9→20 Å / Num. obs: 63215 / Observed criterion σ(I): 1
Reflection shellResolution: 2.9→2.974 Å / % possible all: 81

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Processing

Software
NameVersionClassification
MOSFLMdata reduction
SCALAdata scaling
AMoREphasing
REFMAC5.2refinement
CCP4(SCALA)data scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.05→20 Å / Cor.coef. Fo:Fc: 0.896 / Cor.coef. Fo:Fc free: 0.897 / SU B: 59.115 / SU ML: 0.451 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / ESU R Free: 0.575 / Stereochemistry target values: MAXIMUM LIKELIHOOD
RfactorNum. reflection% reflectionSelection details
Rfree0.29493 2885 5 %RANDOM
Rwork0.27589 ---
obs0.28784 54446 86.53 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK
Displacement parametersBiso mean: 33.43 Å2
Baniso -1Baniso -2Baniso -3
1--1.78 Å20 Å20.55 Å2
2--1.86 Å20 Å2
3---0.05 Å2
Refinement stepCycle: LAST / Resolution: 3.05→20 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms19271 240 342 20 19873
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0060.02220163
X-RAY DIFFRACTIONr_bond_other_d
X-RAY DIFFRACTIONr_angle_refined_deg1.0492.00427253
X-RAY DIFFRACTIONr_angle_other_deg
X-RAY DIFFRACTIONr_dihedral_angle_1_deg5.04352423
X-RAY DIFFRACTIONr_dihedral_angle_2_deg38.74124.189888
X-RAY DIFFRACTIONr_dihedral_angle_3_deg17.536153731
X-RAY DIFFRACTIONr_dihedral_angle_4_deg12.79115162
X-RAY DIFFRACTIONr_chiral_restr0.0610.23131
X-RAY DIFFRACTIONr_gen_planes_refined0.0040.0214686
X-RAY DIFFRACTIONr_gen_planes_other
X-RAY DIFFRACTIONr_nbd_refined0.2060.29802
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined0.2980.213711
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined0.1740.2629
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined0.2070.251
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined0.1180.23
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_mcbond_it0.0021.512578
X-RAY DIFFRACTIONr_mcbond_other
X-RAY DIFFRACTIONr_mcangle_it0.002219632
X-RAY DIFFRACTIONr_scbond_it0.00538449
X-RAY DIFFRACTIONr_scangle_it0.0084.57621
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
LS refinement shellResolution: 3.05→3.127 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.455 133 -
Rwork0.471 2505 -
obs--83.79 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
14.8535-0.7402-2.03221.9041-0.76525.6001-0.2990.2614-0.3272-0.03640.27050.04071.21260.55550.02850.3094-0.08440.00280.0557-0.13780.4622-12.7176-56.692-9.967
28.0032-1.65092.27810.6184-0.11661.0978-0.4673-0.4593-0.5469-0.2168-0.07161.06330.0898-0.57820.5390.1363-0.20330.03050.00220.00280.848-47.5334-34.958819.1134
32.9471.64-1.34326.99570.59425.6232-0.16010.0490.07640.08430.22260.8252-0.26320.0147-0.0625-0.26310.13340.00790.01430.13370.2851-52.660413.400728.1488
46.64320.8199-3.00125.75741.20855.40180.28890.55921.1654-0.3941-0.06820.4562-0.5704-0.2285-0.22070.22210.2569-0.0769-0.2590.13210.0082-16.843747.186621.0213
56.9151-2.7975-3.44232.83532.35323.42380.25330.27960.8065-0.0379-0.0904-0.5362-0.43730.6057-0.16290.3932-0.05360.3632-0.07010.19230.182429.653933.80788.3116
63.10430.3793-1.162614.5753.84663.0721-0.05540.1238-0.4836-0.60690.416-1.18410.5314-0.1021-0.36060.01090.050.0937-0.0305-0.11180.002945.2809-12.770310.2366
71.4879-0.6622-0.13722.34760.55994.9496-0.10930.1769-0.10490.0441-0.02150.35270.0096-0.46620.1309-0.1099-0.15050.0099-0.28640.07870.0464-25.1103-44.20017.2309
81.61550.56790.16022.3240.79052.28770.00570.254-0.2236-0.3307-0.02130.90590.0402-0.19270.0156-0.13340.01970.03390.0964-0.02990.4539-48.1786-13.429926.1169
93.20331.03061.18071.37690.23993.0803-0.26140.53280.5282-0.250.32580.574-0.2619-0.296-0.0644-0.23540.2096-0.0771-0.03640.0572-0.0059-34.893328.046227.8675
103.6083-0.69350.62262.07950.08131.33040.33570.82920.5001-0.7536-0.1658-0.4609-0.3884-0.0894-0.16990.28170.16830.1899-0.23130.1344-0.07645.17841.057318.4421
110.5985-0.55961.16842.649-1.04955.03480.2150.19690.3977-0.66520.0312-0.3794-0.16040.1826-0.2462-0.1937-0.00310.23320.01070.0053-0.095437.7113.071812.2176
123.91380.28941.55091.61070.664.2239-0.0875-0.0257-0.4998-0.29790.0211-0.11010.4576-0.40890.06640.16510.0988-0.1039-0.2382-0.1418-0.041135.4737-28.616223.752
132.2773-0.05150.32043.00480.27533.7554-0.033-0.310.0430.37560.136-0.36830.24930.4823-0.1031-0.17260.1058-0.0031-0.20350.01790.0007-9.0905-33.368625.4197
142.18210.23520.2833.24950.84762.58510.0351-0.3015-0.18840.67540.03130.05630.28140.1119-0.0664-0.2334-0.00050.1392-0.20650.0149-0.0974-27.0721-12.349342.2763
151.80610.93680.55953.56640.77983.1568-0.0361-0.218-0.18170.40910.06110.23360.1436-0.0463-0.025-0.39450.19530.072-0.2686-0.0328-0.5014-19.064318.439546.8031
163.9845-0.04710.43812.1366-0.1012.63410.0379-0.48430.23870.41520.184-0.6346-0.21920.099-0.2219-0.23960.06210.011-0.3508-0.1152-0.319110.512930.211241.7794
173.0895-0.52960.50782.4033-0.8413.30150.0456-0.43390.3220.42610.12-0.3942-0.17020.122-0.1656-0.43990.01740.0253-0.1731-0.1942-0.225237.148912.269538.5922
181.8073-0.28540.44062.8198-0.93753.30250.0964-0.4528-0.27790.25030.04050.04870.25010.0119-0.1369-0.0510.0821-0.1677-0.0193-0.0711-0.240939.2333-18.423647.9121
190.852.0778-2.40888.1197-2.686910.19630.8786-0.5536-0.10831.2787-1.42091.1395-0.35541.22650.54230.60190.3393-0.29920.58170.03270.74250.8657-46.449847.6915
209.72620.30146.93196.528410.28620.4991-0.7103-0.30161.4501-0.75970.4886-0.3062-0.98540.20510.22180.8095-0.13950.16790.40810.19820.4261-26.7523-18.413169.099
2121.1904-19.3994-1.958418.42754.367310.1054-0.46530.43950.73090.39640.44540.3070.36532.07840.01990.3196-0.08950.16030.43940.0842-0.3221-19.662826.263773.0093
2226.21761.191810.468110.53981.63674.30820.3928-0.08180.18810.5337-1.1298-1.0121-0.36391.13120.7370.52810.1482-0.08040.2305-0.32410.281119.626143.399463.9475
238.8904-5.8977-2.445512.2862-8.846113.7598-0.41710.25460.6278-0.4675-0.0865-1.2065-0.11621.06490.50360.1598-0.1434-0.20970.3342-0.35490.582557.032320.312655.4088
246.9909-1.5215-3.00362.3529-2.41795.9572-0.166-0.1732-1.62040.2863-0.3695-0.14210.8621-0.06460.53550.29350.0463-0.31830.41690.03640.254860.3563-22.499263.9811
2578.7175-20.961225.2051155.8946-14.69737.76515.0919-0.5224-3.84390.3098-2.03796.73540.47464.0491-3.05410.3306-0.08550.12270.202-0.15290.46785.6962-36.151723.2751
2628.664525.498423.17262.3266-27.635777.45112.1246-2.70142.09550.2562-0.0451-0.37724.5849-3.4786-2.07950.22010.1602-0.00810.1553-0.22190.2868-20.287-25.621144.7036
27147.7853-40.7695-73.222638.694616.0394114.2259-0.2609-3.2894-0.5409-0.29881.03950.01341.5102-4.8912-0.77870.216-0.0358-0.04220.30530.04250.2407-25.10397.075754.864
288.5924-20.114-5.7118118.610616.628762.6333-1.7712-0.5352-0.80782.91172.50770.3019-0.857-0.7813-0.73640.13410.17580.0810.06740.11710.1296-0.498531.618352.1148
29134.501977.332146.383136.54819.500351.64052.7385-1.72690.52813.5984-2.7755-1.1398-3.6632-1.89020.03690.2423-0.00190.1540.1877-0.06150.285533.086325.160845.2385
30283.921815.904362.76351.44011.26123.13190.9392-0.63124.88950.7401-1.13261.5649-0.70790.62030.19340.28030.05460.00180.101-0.21440.111247.6932-5.844748.0948
310000000000000000.01710.0309-0.00560.0130.01520.01811.0557-33.59918.4731
320000000000000000.01250.0003-0.0024-0.00560.02040.0041-22.1731-22.01237.5333
330000000000000000.0023-0.00770.0094-0.0012-0.00050.002-24.24239.906147.1923
340000000000000000.00760.0078-0.00330.0105-0.00620.0094-1.744729.190445.4103
350000000000000000.00030.00020.00070.000200.000229.585121.98738.2423
36000000000000000-0.00880.0012-0.01910.01610.00860.021441.7393-7.690344.0323
37594.8557-132.226792.0609165.764510.2270.7835-1.9751.46893.071-1.52653.4383-1.01584.42394.386-1.4633-0.0028-0.14510.05670.1249-0.12720.0376-17.0379-20.568634.5414
38131.6617-73.8323-28.0405397.5977-105.387747.1524.56672.85353.63573.1241-6.15121.485.5396-1.0131.58450.06360.3936-0.25020.256-0.1194-0.0173-20.41223.295844.642
39176.097114.5842-110.9036157.3889-15.3452108.8209-3.0842-5.8303-0.1858-0.7516-6.0026-10.30863.73691.66769.08680.06770.0164-0.0797-0.0330.05230.0449-2.332822.779544.6399
40195.5872-52.81327.0913127.270968.644354.8086-7.44011.67343.0273.95252.4646-0.98364.7222-4.17574.97560.0616-0.10230.22350.04690.21350.104123.754218.948341.66
4168.1408-12.5312-19.5072581.0687214.5826240.05622.58893.64150.71055.9004-6.0321-6.03250.5797-2.46973.44320.11920.20790.23270.2172-0.1404-0.005336.8405-3.875945.2434
4225.1336-15.229331.589918.9818-29.788751.32730.33411.26471.8298-0.0401-0.83081.9892-1.29853.01480.49680.0208-0.1009-0.1433-0.0602-0.0404-0.0037-24.205-36.57-7.5834
43298.05748.075195.106155.9636-4.7278102.3434-4.9854.57842.71961.07490.9002-0.933-3.89752.65914.08490.35460.15660.3099-0.0343-0.14310.3371-43.1916-13.33218.5606
4471.23596.106520.849731.648949.234578.43040.0983-0.8377-0.7907-2.63310.53062.07224.2752-0.9564-0.62890.0208-0.02150.21570.39770.16570.2232-36.662817.336414.4106
4517.51810.628839.9098111.2439-110.5892264.32930.1542-0.02262.7801-0.09923.11460.91283.097-1.4093-3.2688-0.0222-0.00190.02790.2715-0.16130.3357-6.235832.53849.4617
4643.37526.7871-54.771829.3309-1.2389120.88961.45282.7678-0.6994-0.6988-0.4720.14771.7029-7.4423-0.98080.5418-0.0698-0.06870.51220.21260.317722.262113.67245.1592
4738.5773-26.149522.9645110.877793.832152.14471.5617-2.32571.7511.4464-0.5709-2.61760.79740.3236-0.99090.4351-0.00620.0120.43820.08940.246624.7346-19.247614.2869
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
1X-RAY DIFFRACTION1AG1 - 461 - 46
2X-RAY DIFFRACTION2BH1 - 461 - 46
3X-RAY DIFFRACTION3CI1 - 461 - 46
4X-RAY DIFFRACTION4DJ1 - 461 - 46
5X-RAY DIFFRACTION5EK1 - 461 - 46
6X-RAY DIFFRACTION6FL1 - 461 - 46
7X-RAY DIFFRACTION7AG47 - 15547 - 155
8X-RAY DIFFRACTION8BH47 - 15547 - 155
9X-RAY DIFFRACTION9CI47 - 15547 - 155
10X-RAY DIFFRACTION10DJ47 - 15547 - 155
11X-RAY DIFFRACTION11EK47 - 15547 - 155
12X-RAY DIFFRACTION12FL47 - 15547 - 155
13X-RAY DIFFRACTION13AG156 - 400156 - 400
14X-RAY DIFFRACTION14BH156 - 400156 - 400
15X-RAY DIFFRACTION15CI156 - 400156 - 400
16X-RAY DIFFRACTION16DJ156 - 400156 - 400
17X-RAY DIFFRACTION17EK156 - 400156 - 400
18X-RAY DIFFRACTION18FL156 - 400156 - 400
19X-RAY DIFFRACTION19AG401 - 417401 - 417
20X-RAY DIFFRACTION20BH401 - 417401 - 417
21X-RAY DIFFRACTION21CI401 - 417401 - 417
22X-RAY DIFFRACTION22DJ401 - 417401 - 417
23X-RAY DIFFRACTION23EK401 - 417401 - 417
24X-RAY DIFFRACTION24FL401 - 417401 - 417
25X-RAY DIFFRACTION25AS16001
26X-RAY DIFFRACTION26BT26001
27X-RAY DIFFRACTION27CV36001
28X-RAY DIFFRACTION28DX46001
29X-RAY DIFFRACTION29EZ56001
30X-RAY DIFFRACTION30EBA66001
31X-RAY DIFFRACTION31AM16011
32X-RAY DIFFRACTION32BN26011
33X-RAY DIFFRACTION33CO36011
34X-RAY DIFFRACTION34DP46011
35X-RAY DIFFRACTION35EQ56011
36X-RAY DIFFRACTION36FR66011
37X-RAY DIFFRACTION37BU27011
38X-RAY DIFFRACTION38CW37011
39X-RAY DIFFRACTION39DY47011
40X-RAY DIFFRACTION40EAA57011
41X-RAY DIFFRACTION41FCA67011
42X-RAY DIFFRACTION42GA1 - 26 - 7
43X-RAY DIFFRACTION43MB1 - 26 - 7
44X-RAY DIFFRACTION44HC1 - 26 - 7
45X-RAY DIFFRACTION45JD1 - 26 - 7
46X-RAY DIFFRACTION46KE1 - 26 - 7
47X-RAY DIFFRACTION47LF1 - 26 - 7

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