[English] 日本語
![](img/lk-miru.gif)
- PDB-1x67: Solution structure of the cofilin homology domain of HIP-55 (dreb... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1x67 | ||||||
---|---|---|---|---|---|---|---|
Title | Solution structure of the cofilin homology domain of HIP-55 (drebrin-like protein) | ||||||
![]() | Drebrin-like protein | ||||||
![]() | PROTEIN BINDING / cell-free protein synthesis / actin-binding protein / SH3P7 / mAbp1 / T-cell lymphocyte signaling and regulation / T-cell antigen receptor regulation / HPK-1 activation / c-JUN N-terminal kinase activation / Structural Genomics / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | ![]() : / site of polarized growth / : / podosome assembly / postsynaptic actin cytoskeleton organization / ruffle assembly / clathrin-coated vesicle membrane / positive regulation of dendritic spine morphogenesis / regulation of actin filament polymerization / podosome ...: / site of polarized growth / : / podosome assembly / postsynaptic actin cytoskeleton organization / ruffle assembly / clathrin-coated vesicle membrane / positive regulation of dendritic spine morphogenesis / regulation of actin filament polymerization / podosome / Neurexins and neuroligins / cortical actin cytoskeleton / Rac protein signal transduction / Caspase-mediated cleavage of cytoskeletal proteins / positive regulation of axon extension / neuron projection morphogenesis / enzyme activator activity / ruffle / synapse assembly / receptor-mediated endocytosis / actin filament binding / tertiary granule lumen / lamellipodium / actin binding / cell cortex / postsynaptic membrane / perikaryon / secretory granule lumen / adaptive immune response / ficolin-1-rich granule lumen / postsynaptic density / early endosome / cadherin binding / protein domain specific binding / Golgi membrane / dendrite / Neutrophil degranulation / extracellular exosome / extracellular region / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
![]() | Goroncy, A.K. / Kigawa, T. / Koshiba, S. / Sato, M. / Kobayashi, N. / Tochio, N. / Inoue, M. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
![]() | ![]() Title: NMR solution structures of actin depolymerizing factor homology domains. Authors: Goroncy, A.K. / Koshiba, S. / Tochio, N. / Tomizawa, T. / Sato, M. / Inoue, M. / Watanabe, S. / Hayashizaki, Y. / Tanaka, A. / Kigawa, T. / Yokoyama, S. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 828.5 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 700 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 1udmC ![]() 1v6fC ![]() 1wfsC ![]() 2d8bC C: citing same article ( |
---|---|
Similar structure data | |
Other databases |
-
Links
-
Assembly
Deposited unit | ![]()
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-
Components
#1: Protein | Mass: 15403.144 Da / Num. of mol.: 1 / Fragment: cofilin homology domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-
Sample preparation
Details | Contents: 1.33mM COFILIN HOMOLOGY DOMAIN, 20mM TRIS-HCL, 100mM NaCl, 1mM d-DTT, 0.02% NaN3 Solvent system: 90% H2O/10% D2O |
---|---|
Sample conditions | Ionic strength: 120mM / pH: 7 / Pressure: ambient / Temperature: 296 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 900 MHz |
---|
-
Processing
NMR software |
| ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: fewest violations | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 |