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Yorodumi- PDB-1x4q: Solution structure of PWI domain in U4/U6 small nuclear ribonucle... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1x4q | ||||||
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Title | Solution structure of PWI domain in U4/U6 small nuclear ribonucleoprotein Prp3(hPrp3) | ||||||
Components | U4/U6 small nuclear ribonucleoprotein Prp3 | ||||||
Keywords | RNA BINDING PROTEIN / PWI domain / Structural Genomics / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information RNA splicing, via transesterification reactions / U2-type precatalytic spliceosome / spliceosomal tri-snRNP complex assembly / Cajal body / U4/U6 x U5 tri-snRNP complex / mRNA Splicing - Major Pathway / RNA splicing / spliceosomal complex / mRNA splicing, via spliceosome / mRNA processing ...RNA splicing, via transesterification reactions / U2-type precatalytic spliceosome / spliceosomal tri-snRNP complex assembly / Cajal body / U4/U6 x U5 tri-snRNP complex / mRNA Splicing - Major Pathway / RNA splicing / spliceosomal complex / mRNA splicing, via spliceosome / mRNA processing / nuclear speck / RNA binding / nucleoplasm / identical protein binding / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | He, F. / Muto, Y. / Inoue, M. / Kigawa, T. / Shirouzu, M. / Terada, T. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be Published Title: Solution structure of PWI domain in U4/U6 small nuclear ribonucleoprotein Prp3(hPrp3) Authors: He, F. / Muto, Y. / Inoue, M. / Kigawa, T. / Shirouzu, M. / Terada, T. / Yokoyama, S. | ||||||
History |
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Remark 650 | HELIX Determination method: author determined |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1x4q.cif.gz | 535.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1x4q.ent.gz | 466.8 KB | Display | PDB format |
PDBx/mmJSON format | 1x4q.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/x4/1x4q ftp://data.pdbj.org/pub/pdb/validation_reports/x4/1x4q | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9911.252 Da / Num. of mol.: 1 / Fragment: PWI domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Description: Cell free protein synthesis / Gene: PRPF3, HPRP3, PRP3 / Plasmid: P040329-14 / References: UniProt: O43395 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.8mM U-15, 13C; 20mM phosphate buffer NA; 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 90% H2O,10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 120mM / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 700 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function, structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |