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Yorodumi- PDB-1x2x: NMR solution structures of a DNA dodecamer containing a tandem GT... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1x2x | ||||||||||||||||||
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Title | NMR solution structures of a DNA dodecamer containing a tandem GT mismatches using NOE and residual dipolar couplings | ||||||||||||||||||
Components | 5'-D(*Keywords | DNA / RDC / deoxyribonucleic acid / liquid crystal / tandem GT mismatches | Function / homology | DNA / DNA (> 10) | Function and homology information Method | SOLUTION NMR / simulated annealing, energy minimization | Authors | Alvarez-Salgado, F. / Desvaux, H. / Boulard, Y. | Citation | Journal: Magn.Reson.Chem. / Year: 2006 | Title: NMR assessment of the global shape of a non-labelled DNA dodecamer containing a tandem of G--T mismatches Authors: Alvarez-Salgado, F. / Desvaux, H. / Boulard, Y. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1x2x.cif.gz | 22.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1x2x.ent.gz | 16 KB | Display | PDB format |
PDBx/mmJSON format | 1x2x.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/x2/1x2x ftp://data.pdbj.org/pub/pdb/validation_reports/x2/1x2x | HTTPS FTP |
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-Related structure data
Related structure data | 1x2oC 1x2sC 1x2uC 1x2vC 1x2yC 1x2zC 1x30C C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: DNA chain | Mass: 3678.403 Da / Num. of mol.: 2 / Source method: obtained synthetically |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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NMR details | Text: RDCs were measured in bicelles SDS solution |
-Sample preparation
Details | Contents: 0.5mM DNA, 10mM PHOSPHATE BUFFER, 50mM NACL, 0.5mM EDTA |
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Sample conditions | Ionic strength: 10mM PHOSPHATE BUFFER, 50mM NACL, 0.2mM EDTA pH: 7.0 / Pressure: AMBIENT / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing, energy minimization / Software ordinal: 1 | ||||||||||||||||||||
NMR ensemble | Conformers submitted total number: 1 |