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- PDB-1x02: Solution structure of stereo array isotope labeled (SAIL) calmodulin -
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Open data
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Basic information
Entry | Database: PDB / ID: 1x02 | ||||||
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Title | Solution structure of stereo array isotope labeled (SAIL) calmodulin | ||||||
![]() | calmodulin | ||||||
![]() | METAL BINDING PROTEIN / SAIL / stereo array isotope labeling | ||||||
Function / homology | ![]() enzyme regulator activity / signaling receptor binding / calcium ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
![]() | Kainosho, M. / Torizawa, T. / Terauchi, T. / Ono, A.M. / Guntert, P. | ||||||
![]() | ![]() Title: Optimal isotope labelling for NMR protein structure determinations. Authors: Kainosho, M. / Torizawa, T. / Iwashita, Y. / Terauchi, T. / Mei Ono, A. / Guntert, P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 883.4 KB | Display | ![]() |
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PDB format | ![]() | 743.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 339.1 KB | Display | ![]() |
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Full document | ![]() | 498.2 KB | Display | |
Data in XML | ![]() | 65.5 KB | Display | |
Data in CIF | ![]() | 81.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 16721.350 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-CA / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 0.7mM SAIL calmodulin, 5mM MES-d13, 10mM bis-Tris-d19, 5mM CaCl2, 0.1mM NaN3 Solvent system: 90% H2O/10% D2O |
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Sample conditions | pH: 6.5 / Pressure: AMBIENT / Temperature: 310 K |
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Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 |