+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1wum | ||||||
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タイトル | Complex structure of PCAF bromodomain with small chemical ligand NP2 | ||||||
要素 | Histone acetyltransferase PCAF | ||||||
キーワード | TRANSFERASE (転移酵素) / BROMODOMAIN (ブロモドメイン) / HISTONE-ACETYLTRANSFERASE / NMR-STRUCTURE / CHEMICAL LIGAND / NP2 / NP1 | ||||||
機能・相同性 | 機能・相同性情報 regulation of protein ADP-ribosylation / negative regulation of rRNA processing / histone H3K9 acetyltransferase activity / diamine N-acetyltransferase / diamine N-acetyltransferase activity / positive regulation of transcription from RNA polymerase II promoter by glucose / negative regulation of centriole replication / Physiological factors / positive regulation of attachment of mitotic spindle microtubules to kinetochore / peptidyl-lysine acetylation ...regulation of protein ADP-ribosylation / negative regulation of rRNA processing / histone H3K9 acetyltransferase activity / diamine N-acetyltransferase / diamine N-acetyltransferase activity / positive regulation of transcription from RNA polymerase II promoter by glucose / negative regulation of centriole replication / Physiological factors / positive regulation of attachment of mitotic spindle microtubules to kinetochore / peptidyl-lysine acetylation / lysine N-acetyltransferase activity, acting on acetyl phosphate as donor / YAP1- and WWTR1 (TAZ)-stimulated gene expression / positive regulation of fatty acid biosynthetic process / ミオフィラメント / internal peptidyl-lysine acetylation / histone H3 acetyltransferase activity / cyclin-dependent protein serine/threonine kinase inhibitor activity / N-terminal peptidyl-lysine acetylation / ATAC complex / I band / cellular response to parathyroid hormone stimulus / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / RUNX3 regulates NOTCH signaling / SAGA complex / limb development / A band / NOTCH4 Intracellular Domain Regulates Transcription / Regulation of FOXO transcriptional activity by acetylation / NOTCH3 Intracellular Domain Regulates Transcription / regulation of tubulin deacetylation / peptide-lysine-N-acetyltransferase activity / histone acetyltransferase binding / protein acetylation / Notch-HLH transcription pathway / Formation of WDR5-containing histone-modifying complexes / regulation of cell division / Formation of paraxial mesoderm / acetyltransferase activity / RNA Polymerase I Transcription Initiation / regulation of RNA splicing / regulation of embryonic development / regulation of DNA repair / histone acetyltransferase activity / positive regulation of gluconeogenesis / ヒストンアセチルトランスフェラーゼ / transcription initiation-coupled chromatin remodeling / positive regulation of glycolytic process / 糖新生 / transcription coregulator activity / RNA polymerase II transcription regulatory region sequence-specific DNA binding / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / B-WICH complex positively regulates rRNA expression / NOTCH1 Intracellular Domain Regulates Transcription / Metalloprotease DUBs / 紡錘体 / 記憶 / 動原体 / Pre-NOTCH Transcription and Translation / Constitutive Signaling by NOTCH1 PEST Domain Mutants / Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants / positive regulation of neuron projection development / histone deacetylase binding / cellular response to insulin stimulus / vasodilation / rhythmic process / cellular response to oxidative stress / heart development / HATs acetylate histones / DNA-binding transcription factor binding / Estrogen-dependent gene expression / transcription coactivator activity / regulation of cell cycle / クロマチンリモデリング / 細胞周期 / negative regulation of cell population proliferation / 中心体 / chromatin binding / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / protein kinase binding / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / protein-containing complex / 核質 / 細胞核 / 細胞質基質 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / distance geometry | ||||||
データ登録者 | Zeng, L. / Li, J. / Muller, M. / Yan, S. / Mujtaba, S. / Pan, C. / Wang, Z. / Zhou, M.M. | ||||||
引用 | ジャーナル: J.Am.Chem.Soc. / 年: 2005 タイトル: Selective small molecules blocking HIV-1 Tat and coactivator PCAF association 著者: Zeng, L. / Li, J. / Muller, M. / Yan, S. / Mujtaba, S. / Pan, C. / Wang, Z. / Zhou, M.M. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1wum.cif.gz | 51 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1wum.ent.gz | 36.3 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1wum.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/wu/1wum ftp://data.pdbj.org/pub/pdb/validation_reports/wu/1wum | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 14052.226 Da / 分子数: 1 / 断片: BROMODOMAIN / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / プラスミド: PET14B / 生物種 (発現宿主): Escherichia coli / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 株 (発現宿主): BL21(DE3) 参照: UniProt: Q92831, ヒストンアセチルトランスフェラーゼ |
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#2: 化合物 | ChemComp-NP2 / |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||
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NMR実験 |
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NMR実験の詳細 | Text: HNCACB, HN(CO)CACB, HCCH_TOCSY, (H)C(CO)NH_TOCSY, HNHA, 2D_1H_ROESY |
-試料調製
詳細 | 内容: 0.5mM sample in 100mM phosphate buffer containing 5mM perdeuterated DTT 溶媒系: 10% D2O |
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試料状態 | pH: 6.5 / 温度: 303 K |
-NMR測定
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M | |||||||||||||||
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放射波長 | 相対比: 1 | |||||||||||||||
NMRスペクトロメーター |
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-解析
NMR software |
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精密化 | 手法: distance geometry / ソフトェア番号: 1 / 詳細: SIMULATED ANNEALING | ||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the least restraint violations 計算したコンフォーマーの数: 200 / 登録したコンフォーマーの数: 1 |