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Yorodumi- PDB-1wry: Solution structure of the SH3 domain-binding glutamic acid-rich-l... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1wry | ||||||
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Title | Solution structure of the SH3 domain-binding glutamic acid-rich-like protein | ||||||
Components | SH3 domain-binding glutamic acid-rich-like protein | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / SH3BGR like protein / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information protein-RNA adaptor activity / positive regulation of cytoplasmic translational initiation / ubiquitin-like ligase-substrate adaptor activity / SH3 domain binding / proteasome-mediated ubiquitin-dependent protein catabolic process / extracellular space / extracellular exosome / nucleus / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Inoue, K. / Miyamoto, K. / Nagashima, T. / Hayashi, F. / Kigawa, T. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be Published Title: Solution structure of the SH3 domain-binding glutamic acid-rich-like protein Authors: Inoue, K. / Miyamoto, K. / Nagashima, T. / Hayashi, F. / Kigawa, T. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1wry.cif.gz | 694 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1wry.ent.gz | 603.8 KB | Display | PDB format |
PDBx/mmJSON format | 1wry.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wr/1wry ftp://data.pdbj.org/pub/pdb/validation_reports/wr/1wry | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13124.399 Da / Num. of mol.: 1 / Fragment: SH3BGR domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Description: Cell-free protein synthesis / Gene: IMS cDNA STM03594 / Plasmid: P040517-09 / References: UniProt: O75368 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.10mM SH3BGR U-15N,13C; 20mM d-Tris-HCl; 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 120mM / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function,structures with the lowest energy,structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 |