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Yorodumi- PDB-1wfs: Solution Structure of Glia Maturation Factor-gamma from Mus Musculus -
+Open data
-Basic information
Entry | Database: PDB / ID: 1wfs | ||||||
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Title | Solution Structure of Glia Maturation Factor-gamma from Mus Musculus | ||||||
Components | Glia maturation factor gamma | ||||||
Keywords | PROTEIN BINDING / ACTIN BINDING PROTEIN / CYTOSKELETON / STRUCTURAL GENOMICS / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information actin filament debranching / Arp2/3 complex binding / negative regulation of Arp2/3 complex-mediated actin nucleation / Neutrophil degranulation / growth factor activity / actin binding Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Goroncy, A.K. / Kigawa, T. / Koshiba, S. / Kobayashi, N. / Tochio, N. / Inoue, M. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: Protein Sci. / Year: 2009 Title: NMR solution structures of actin depolymerizing factor homology domains. Authors: Goroncy, A.K. / Koshiba, S. / Tochio, N. / Tomizawa, T. / Sato, M. / Inoue, M. / Watanabe, S. / Hayashizaki, Y. / Tanaka, A. / Kigawa, T. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1wfs.cif.gz | 1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1wfs.ent.gz | 904.9 KB | Display | PDB format |
PDBx/mmJSON format | 1wfs.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1wfs_validation.pdf.gz | 343.9 KB | Display | wwPDB validaton report |
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Full document | 1wfs_full_validation.pdf.gz | 521.9 KB | Display | |
Data in XML | 1wfs_validation.xml.gz | 70.8 KB | Display | |
Data in CIF | 1wfs_validation.cif.gz | 87.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wf/1wfs ftp://data.pdbj.org/pub/pdb/validation_reports/wf/1wfs | HTTPS FTP |
-Related structure data
Related structure data | 1udmC 1v6fC 1x67C 2d8bC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 17180.508 Da / Num. of mol.: 1 / Fragment: COFILIN-ADF-domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Description: Cell-free protein synthesis / Gene: RIKEN cDNA 2310057N07 / Plasmid: P030324-83 / References: UniProt: Q9ERL7 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.21mM cofilin U-15N,13C; 20mM phosphate buffer Na; 100mM NaCl; 0.02% NaN3; 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 120mM / pH: 6.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz |
-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest target function | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 |