Mass: 18.015 Da / Num. of mol.: 398 / Source method: isolated from a natural source / Formula: H2O
Compound details
ENGINEERED MUTATION CYS 70 GLU CHAINS A AND B.
Sequence details
THE PROTEIN IS PURIFIED USING AN N-TERMINAL HIS-TAG, WHICH IS CLEAVED OFF PRIOR TO CRYSTALLIZATION. ...THE PROTEIN IS PURIFIED USING AN N-TERMINAL HIS-TAG, WHICH IS CLEAVED OFF PRIOR TO CRYSTALLIZATION. THROMBIN CLEAVAGE REMOVES AN INCOMPLETE FRAGMENT OF THE HIS-TAG, LEAVING THREE RESIDUES ATTACHED TO THE INTENDED PROTEIN SEQUENCE
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION
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Sample preparation
Crystal
Density Matthews: 2.61 Å3/Da / Density % sol: 52.93 %