+Open data
-Basic information
Entry | Database: PDB / ID: 1viw | ||||||||||||
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Title | TENEBRIO MOLITOR ALPHA-AMYLASE-INHIBITOR COMPLEX | ||||||||||||
Components |
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Keywords | COMPLEX (GLYCOSIDASE/INHIBITOR) / COMPLEX (GLYCOSIDASE-INHIBITOR) / HYDROLASE / LECTIN / INSECT ALPHA-AMYLASE / INHIBITORS / COMPLEX (GLYCOSIDASE-INHIBITOR) complex | ||||||||||||
Function / homology | Function and homology information alpha-amylase inhibitor activity / alpha-amylase / carbohydrate catabolic process / alpha-amylase activity / chloride ion binding / carbohydrate binding / calcium ion binding Similarity search - Function | ||||||||||||
Biological species | Tenebrio molitor (yellow mealworm) Phaseolus vulgaris (French bean) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3 Å | ||||||||||||
Authors | Nahoum, V. / Egloff, M.P. / Payan, F. | ||||||||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 1999 Title: A plant-seed inhibitor of two classes of alpha-amylases: X-ray analysis of Tenebrio molitor larvae alpha-amylase in complex with the bean Phaseolus vulgaris inhibitor. Authors: Nahoum, V. / Farisei, F. / Le-Berre-Anton, V. / Egloff, M.P. / Rouge, P. / Poerio, E. / Payan, F. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1viw.cif.gz | 140.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1viw.ent.gz | 108.6 KB | Display | PDB format |
PDBx/mmJSON format | 1viw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1viw_validation.pdf.gz | 533.5 KB | Display | wwPDB validaton report |
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Full document | 1viw_full_validation.pdf.gz | 575.7 KB | Display | |
Data in XML | 1viw_validation.xml.gz | 20.9 KB | Display | |
Data in CIF | 1viw_validation.cif.gz | 30.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vi/1viw ftp://data.pdbj.org/pub/pdb/validation_reports/vi/1viw | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 51233.039 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: LARVAE / Source: (natural) Tenebrio molitor (yellow mealworm) / References: UniProt: P56634, alpha-amylase | ||||
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#2: Protein | Mass: 22714.693 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Phaseolus vulgaris (French bean) / Organ: SEED / References: UniProt: P02873 | ||||
#3: Polysaccharide | Source method: isolated from a genetically manipulated source #4: Chemical | ChemComp-CL / | #5: Chemical | ChemComp-CA / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 53 % Description: PPA IS PORCINE PANCREATIC ALPHA-AMYLASE AND ALPHA-AI PHASEOLUS VULGARIS INHIBITOR | ||||||||||||||||||||||||
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Crystal grow | pH: 7 / Details: pH 7 | ||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: LURE / Beamline: DW32 / Wavelength: 0.97 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE AREA DETECTOR / Date: Nov 1, 1997 |
Radiation | Monochromator: Y / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97 Å / Relative weight: 1 |
Reflection | Resolution: 3→10 Å / Num. obs: 14982 / % possible obs: 99.3 % / Observed criterion σ(I): 1 / Redundancy: 3 % / Biso Wilson estimate: 38.1 Å2 / Rmerge(I) obs: 0.153 / Net I/σ(I): 4.1 |
Reflection shell | Resolution: 3→3.1 Å / Redundancy: 2.9 % / Rmerge(I) obs: 0.45 / Mean I/σ(I) obs: 1.3 / % possible all: 99.3 |
Reflection | *PLUS Num. measured all: 45403 |
Reflection shell | *PLUS % possible obs: 99.3 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PPA AND ALPHA-AI Resolution: 3→10 Å / Data cutoff high absF: 1000000 / Data cutoff low absF: 1.0E-5 / σ(F): 1
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Displacement parameters | Biso mean: 35.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.55 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3→3.07 Å / Total num. of bins used: 15
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