+Open data
-Basic information
Entry | Database: PDB / ID: 1vid | ||||||
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Title | CATECHOL O-METHYLTRANSFERASE | ||||||
Components | CATECHOL O-METHYLTRANSFERASE | ||||||
Keywords | TRANSFERASE (METHYLTRANSFERASE) / TRANSFERASE / METHYLTRANSFERASE / NEUROTRANSMITTER DEGRADATION | ||||||
Function / homology | Function and homology information Enzymatic degradation of dopamine by COMT / Enzymatic degradation of Dopamine by monoamine oxidase / positive regulation of homocysteine metabolic process / Methylation / norepinephrine secretion / response to dopamine / mastication / catechol-containing compound metabolic process / catecholamine catabolic process / catechol O-methyltransferase activity ...Enzymatic degradation of dopamine by COMT / Enzymatic degradation of Dopamine by monoamine oxidase / positive regulation of homocysteine metabolic process / Methylation / norepinephrine secretion / response to dopamine / mastication / catechol-containing compound metabolic process / catecholamine catabolic process / catechol O-methyltransferase activity / renal sodium excretion / S-adenosylhomocysteine metabolic process / catechol O-methyltransferase / renal filtration / developmental process / renin secretion into blood stream / dopamine secretion / negative regulation of dopamine metabolic process / renal albumin absorption / catecholamine metabolic process / habituation / artery development / response to salt / short-term memory / S-adenosylmethionine metabolic process / cerebellar cortex morphogenesis / cellular response to phosphate starvation / norepinephrine metabolic process / dopamine catabolic process / glomerulus development / fear response / synaptic transmission, dopaminergic / response to angiotensin / cellular response to cocaine / estrogen metabolic process / exploration behavior / response to food / cholesterol efflux / response to temperature stimulus / response to corticosterone / prostaglandin metabolic process / response to pain / glycogen metabolic process / dopamine metabolic process / startle response / detection of temperature stimulus involved in sensory perception of pain / : / : / behavioral fear response / multicellular organismal response to stress / response to amphetamine / negative regulation of smooth muscle cell proliferation / learning / kidney development / response to cytokine / female pregnancy / regulation of blood pressure / visual learning / multicellular organism growth / response to organic cyclic compound / response to toxic substance / memory / cognition / response to wounding / response to estrogen / gene expression / cell body / postsynaptic membrane / methylation / vesicle / response to oxidative stress / postsynapse / response to lipopolysaccharide / learning or memory / dendritic spine / response to hypoxia / response to xenobiotic stimulus / axon / glutamatergic synapse / dendrite / magnesium ion binding / membrane / cytosol Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2 Å | ||||||
Authors | Vidgren, J. / Svensson, L.A. / Liljas, A. | ||||||
Citation | Journal: Nature / Year: 1994 Title: Crystal structure of catechol O-methyltransferase. Authors: Vidgren, J. / Svensson, L.A. / Liljas, A. #1: Journal: Proteins / Year: 1991 Title: Crystallization and Preliminary X-Ray Investigation of a Recombinant Form of Rat Catechol O-Methyltransferase Authors: Vidgren, J. / Tilgmann, C. / Lundstrom, K. / Liljas, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1vid.cif.gz | 58.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1vid.ent.gz | 42.1 KB | Display | PDB format |
PDBx/mmJSON format | 1vid.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1vid_validation.pdf.gz | 456.7 KB | Display | wwPDB validaton report |
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Full document | 1vid_full_validation.pdf.gz | 458.7 KB | Display | |
Data in XML | 1vid_validation.xml.gz | 6.6 KB | Display | |
Data in CIF | 1vid_validation.cif.gz | 9.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vi/1vid ftp://data.pdbj.org/pub/pdb/validation_reports/vi/1vid | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 24772.400 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Tissue: LIVER / Production host: Escherichia coli (E. coli) / References: UniProt: P22734, catechol O-methyltransferase |
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#2: Chemical | ChemComp-MG / |
#3: Chemical | ChemComp-SAM / |
#4: Chemical | ChemComp-DNC / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.58 Å3/Da / Density % sol: 50 % | |||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 22 ℃ / pH: 6.5 / Method: vapor diffusion, hanging dropDetails: Vidgren, J., (1991) Proteins: Struct.,Funct., Genet., 11, 233. | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: SYNCHROTRON / Site: SRS / Beamline: PX9.5 / Wavelength: 0.91 |
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Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Aug 1, 1993 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.91 Å / Relative weight: 1 |
Reflection | Resolution: 2→15 Å / Num. obs: 16005 / % possible obs: 85 % / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.083 |
Reflection | *PLUS Num. measured all: 158331 |
-Processing
Software |
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Refinement | Resolution: 2→8 Å / σ(F): 0
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Displacement parameters | Biso mean: 1.87 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→8 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 1.87 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_dihedral_angle_d / Dev ideal: 23.8 |